We narrowed to 98 results for: sgRNA

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

CRISPR-Sirius is a new tool to visualize genomic loci using dCas9. It's brighter than its predecessor CRISPRainbow and unlike fluorescent in situ hybridization, can be used in live cells.

Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently available in lentiviral format.

CRISPR Cas9 gRNA plasmids from the Zhang lab

With many different Cas9 variants out there, it can be hard to know which one to use in your particular CRISPR experiment. This post is here to help.

It can be difficult to choose the best CRISPR software tool. The CRISPR Software Matchmaker makes choosing a guide RNA design software easier.

Learn about CRISPR terminology for common DNA, proteins, and RNAs involved in this CRISPR 101 blog post.

Learn about CRISPR activation tools for neuroscience including a SPH transgenic mice, neuron-optimized viral vectors, and high-throughput screening.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Find prime editor plasmids for (search and replace) genome editing.

Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.

This post, written by the creator of the CRISPRecise kit, explains how to use it to minimize off-target effects in CRISPR genome editing.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

New CRISPR pooled libraries, such as SAM, CRISPRa, and CRISPRi, offer new ways to do genome-wide functional screens.

This post provides tips for designing a prime editing experiment, from pegRNA design, PE tool selection, and choice of DNA sequence to edit.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

This post describes applications of pooled libraries, briefly explains several approaches, and highlights the most popular pooled libraries at Addgene.

This blog posts discusses Cas9 gene drives and the appropriate safeguard and confinement strategies scientists should use for these gene drives.

Recent highlights from Addgene's collection include a system to examine biosynthetic pathways, and a new tool for enriching ABE-targeted cells.