We narrowed to 113 results for: PIP

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM...complete containing 10 μg/mL polybrene. Mix well by pipetting or inverting. Aliquot 1.35 mL of the cell suspension...

...Biosafety Cabinet 4 °C Refrigerator Micropipettes and tips Pipettor and pipettes Benchtop centrifuge compatible...Reagent Preparation instructions. Wipe down all pipettes and equipment with 10% bleach prior to use. Reagent...Check the cell density and viability: Using a 5 mL pipette, transfer 0.5 mL of HEK293 cell suspension into...the microfuge tube containing the trypan blue. Pipette 10 times to mix. Load 10 µL of the cell suspension...

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM...DMEM complete + 10 µg/mL polybrene. Mix well by pipetting or inverting the tube. Aliquot 1 mL of cell suspension...

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette CO 2 incubator Pipet controller Hazardous waste container Microscope Hemocytometer... by passing several times through a serological pipet or by passing through a 40 µm cell strainer mesh...

...storage in the cold room may damage it. Using a pipettor, transfer the top, aqueous phase to a new RNAse-free...disturb or collect the interphase layer with your pipette tip. Pro-Tip To collect as much of the top aqueous...interphase layer, consider using a lower volume pipettor like a p200 to collect a majority of the aqueous...

...spread antibiotic over-agar. Equipment Pipette tips for both pipetting and spreading Bunsen burner (or other...diameter petri plate containing solidified LB-agar, pipette 150 µL of carbenicillin on top of the agar and ...liquid remaining on the surface). We use the micropipette tip itself to do the spreading; the tip is gently...same way as the antibiotic, using either a bent micropipette tip or other cell spreading device that fits...

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM...

...is unloaded, push the pipettor to the second stop and carefully raise the pipette straight out of the buffer...comb or towards the sides/edges of the gel with a pipette tip. Place newly poured gel at 4 °C for 10-15 mins... the tip. Place the very top of the tip of the pipette into the buffer just above the well. Very slowly...needed because several microliters can be lost in pipetting. For example, if you want to load 1.0 μg in 10...

...Spreaders (VWR, 60828-680) 5 mL and 10 mL Serological pipettes Ice slurry (Ice bucket with ice and water to create...until completely thawed. Chill a box of 200 µL micropipette tips in a -20 °C freezer. Aliquot 3 mL SOC into... two separate transformations). Pro-Tip Do not pipette repeatedly or mix when removing SOC containing ... Use one scraper for all plates. Use two 10 mL pipettes (one for dispensing cold LB and one for removing...). Pro-Tip Scraped bacteria in LB can clog the pipette either mix gently up and down avoiding introducing...

...be worn at all times. Use plastic pipettes in place of glass pipettes or needles. Liquid waste should be...OPTI-MEM In a polypropylene tube, add OPTI-MEM first. Pipette FuGENE® directly into the OPTI-MEM – do not allow...tube from step c for a total volume of 100 μL. Pipette master mix directly into the liquid and not onto...cells. Swirl to disperse mixture evenly. Do not pipette or swirl too vigorously, as you do not want to ...fresh DMEM + 10% FBS + penicillin/streptomycin. Pipette the media onto the side of the plate so as not ...