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Showing: 361 - 400 of 621 results

  1. dTAG - You're it!

    Type
    Blog Post
    ...proliferation of basal-like breast cancer cells. eLife. September 2017. 6: e26693. PubMed PMID: 28926338. PubMed...Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588.e28 PubMed PMID: 29224777...

  2. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...going to pour these plates now” to a fellow team member. But, at Addgene, we use seven different types ... professional life, let me know that, too. And remember, if you discover during grad school or during ...

  3. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    .... Protein kinases deliver messages to the next members of their pathways, but where the kinases are located...nucleus. But if the inhibitor targeted upstream members of the pathway (Raf1, EGRF), the KTR localized ...

  4. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...Autophagosomes are relatively easy to identify: double-membraned structures containing undigested cytoplasmic contents...stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the...

  5. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as ...resources and protocols! We invite feedback from all members of the research community to help us develop these...

  9. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell types:...oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology...

  10. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...days, weeks, or months. It is always helpful to remember that if a PI asks for assistance in the grant ...of the statements may be slightly far reaching, remember that you are writing a proposal to test hypotheses...

  11. Hot Plasmids February 2024

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    Blog Post
    ...variants:   Figure 2: Inner mitochondrial membranes imaged with structured illumination microscopy...2023). StayGold variants for molecular fusion and membrane-targeting applications. Nature Methods, 1–9. https...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    ...have a half life of 84 hours in primary mouse embryonic fibroblasts. This fusion protein can be used to... The researchers tested several scenarios from embryo development to myeloid proliferation in acute myeloid...

  15. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...sensors available that will localize at the plasma membrane. GCaMP-X is a nucleus-targeted GCaMP6 with an ...the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry on! References and Resources...

  17. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...overlooked regulatory fragments of these transcripts. I remember when small RNA was just something we ran off the...hypothesized that life started with RNA and a simple cell membrane. His recent work shows that they are making fairly...

  18. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... adaptable into future magnetogenetic tools.  Embracing science in the open The York Lab operates with...applications across a wide range of topics. He embraces open science, self-publishing directly on his ...

  19. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...associate with the endogenously expressed SCF complex members, an E3 ubiquitin ligase complex. Finally, when ...Chem Biol. 2015 Sep;11(9):713-20. doi: 10.1038/nchembio Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi...

  20. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...break), write down some notes on the card so you remember who they are and what you’ve talked about. After... Resources on the Addgene website Become of a member of the Addgene depositor community ...

  21. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post
    ...aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots to use for qPCR...Tips When calculating the titer for your AAV, remember to account for the dilution of the sample for ...

  27. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...!). In 1997, Joseph Provost, then a new faculty member at Minnesota State (and now Professor of Chemistry...and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the recipe...

  29. Tips for arabidopsis transformation

    Type
    Blog Post
    ...2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from the Dinneny...Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September 2018). Additional resources on the Addgene blog...

  30. An Introduction to Adenovirus

    Type
    Blog Post
    ... derived from the mastadenovirus members (mammalian-infecting members) of the Adenoviridae family, which...

  31. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...want to know the orientation of the insert. One member of this type of primer pair anneals to a sequence...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read about...

  32. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...nucleus. To circumvent this requirement for transmembrane proteins, a split ubiquitin system has been ...genetic system optimized for systematic studies of membrane protein interactions. Proc Natl Acad Sci U S A...

  34. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...technically gamma-retroviruses - another, separate member of the retroviral family. While both lentiviruses...Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing and non-...

  35. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...Maybe you’ll need to care for an elderly family member, want to train for a triathlon, finally buy that...laundry, take public transportation, call a cab and remember their own school assignments, the better. In my...

  36. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... thereafter, using mouse instead of human AAG (remember, MPG and AAG are the same protein!) (Chen et al...A•T-to-C•G base editing in mammalian cells and embryos. Nature Biotechnology, 42(4), 638–650. https://...

  37. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...interested in working for this lab and/or company. Remember to proofread your cover letter, and if possible... originally written by Maria Soriano-Carot in September 2016 and was updated by Rachel Leeson in May 2024...

  39. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...cleaving genomic DNA in mammalian cells and zebrafish embryos, and they can be used to modify genomic loci that...SpCas9 (Amrani et al., 2018). When choosing a Cas9, remember to check that your tracrRNA and crRNA (or synthetic...

  40. Hot Plasmids - August 2020

    Type
    Blog Post
    ...with plasmids encoding FPs tagged with the plasma membrane targetin sequence derived either from p63(1-29...non-specific labeling. For labeling the plasma membrane, they tested different target sequences for improved...
Showing: 361 - 400 of 621 results