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We narrowed to 731 results for: Sis;

Showing: 361 - 400 of 731 results

  3. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...neuronal circuit tracing.     Marcus Leiwe is an assistant professor at Kyushu University. He has a particular...resolution using chemical cocktails and computational analysis." Cell 157.3 (2014): 726-739. PubMed PMID: 24746791...

  4. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ... dynamic process which involves autophagosome synthesis, delivery of materials to be degraded to the lysosome...mammalian dual-emission autophagy biosensor. Its resistance to lysosomal proteases is key to its pH-dependent...

  5. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...perception of head injury risk. In completing a master’s thesis in science communication, I had the chance to carry...necessary to inform the experimental aspect of my thesis: a survey instrument designed to measure how individuals...

  6. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...Instead, it targets a specific audience - giving consistency, purpose, structure, and intent to the messaging...your audience, and their expectations of you, consistent and clear.   And now that we’re all on the same...

  7. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...detection of the tagged protein. These peptides consist of as little as 8 or as many as several hundred...10.1007/s00253-002-1158-6 Baker, M., Reproducibility crisis: Blame it on the antibodies. Nature (2015) 521,...

  8. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...degree and some marketing experience, with little emphasis placed on the marketing or business background...thought they would focus on “Has this person been consistent throughout their career?”, "Have they sought ...

  9. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...activity to start a discussion. The Forced Choice Analysis is a fun example and you can find it in our Mentoring...series of mentoring blog posts, the "Forced Choice Analysis Activity", the "Peer Mentor Groups Guide", "Tips...

  12. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...controlling for journal. And so, while our quantitative analysis cannot rule out all other explanations, we do ...ssrn.com/author=1232466.     Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT...

  13. Science Career Options

    Type
    Blog Post
    ...Ecologist     Computer and writing skills Writing a thesis or dissertation demonstrates that you have advanced...what each of these roles involves on a dad-to-day basis. Often, it is hard to know what a job title actually...

  14. The MTA at Addgene

    Type
    Blog Post
    ...beginning makes the sharing process smoother, more consistent, and more pleasant for everyone, which in turn...including MTAs, is perfect, but it does provide consistency and transparency in an otherwise messy process...

  16. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ... prepared me for what a PI without a personal assistant has to deal with. My inbox currently stands at...advisor Wally Gilbert: “Very interdisciplinary, with emphasis on genetic engineering, sequencing, and translation...

  17. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the same sequence...:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP. This visual...

  22. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...Less than 20 ng of total gDNA is sufficient for analysis. We have routinely successfully analyzed gDNA .... 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...

  23. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...Institute were instrumental in helping us establish consistent, high-quality protocols for lentiviral production...at Addgene. Our Board helps keep our focus on assisting scientists and accelerating research and discovery...

  25. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...The ITRs base pair with each other to allow DNA synthesis of the complementary strand to yield dsDNA. Unlike...ones listed previously). E3/E1B suppresses both apoptosis and the host immune response to the virus to allow...

  26. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...population is specific for your target antibody and may assist in gating your target population (b). There..., so you can use most of your samples for flow analysis rather than for setting up your initial voltages...

  27. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...emphasizing the importance of critical thinking, persistence, teamwork, and communication. And as a program...breathalyzer to pesticide breakdown, production of fire-resistant housing insulation, and cleaning up salt runoff...

  28. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ... day of the interview, dress the part, and be consistent with your personal statement, while you make ...success. Every student should use these guidelines consistently across a number of years as you apply for internships...

  32. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...journal. An increasing number of funding bodies insist that the research data resulting from their funding...and make up their own minds on whether the data analysis has been of good quality and whether they agree...

  33. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...antibiotic in the plate correspond to the antibiotic resistance marker present in your plasmid. You should also...is due to a PCR mistake or a mistake by the DNA analysis software. Now that you've read our plasmid troubleshooting...

  36. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...systematically and logically is as useful for finishing your thesis as it is for helping your team reach an important...thinking, decision making, creativity, or data analysis. Conveniently, these are all skills you are already...

  37. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...other genetic disorders, pre-implantation genetic diagnosis (PGD) screens embryos to find those that do not...determine its genotype. However, this detailed analysis is not possible with IVF, as most of the cells...

  38. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...the inter-molecular annealing or second strand synthesis. In contrast, a scAAV vector with half the size...promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001). PubMed PMID: 11509958...

  39. All in a Twist: dsRNA

    Type
    Blog Post
    ...centromeres. These genomic regions are prone to persistent R-loop formation, presumably due to their abundance...considerations when using these reagents. RNA:DNA hybrids Persistent RNA:DNA hybrids that arise during transcription...

  40. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...using words for their field or technology in a consistent way. Just creating a lexicon was not enough. ... using the same words with the same meanings consistently, meetings will be shorter and more productive...
Showing: 361 - 400 of 731 results