We narrowed to 731 results for: Sis;

...degree and some marketing experience, with little emphasis placed on the marketing or business background...thought they would focus on “Has this person been consistent throughout their career?”, "Have they sought ...

...activity to start a discussion. The Forced Choice Analysis is a fun example and you can find it in our Mentoring...series of mentoring blog posts, the "Forced Choice Analysis Activity", the "Peer Mentor Groups Guide", "Tips...

...beginning makes the sharing process smoother, more consistent, and more pleasant for everyone, which in turn...including MTAs, is perfect, but it does provide consistency and transparency in an otherwise messy process...

...2017. Pubmed PMID: 16371404 Sniderman et al, Athersclerosis 2012. Pubmed PMID: 23068583 Thierer et al, ...UAS). The Wang Lab created a tripartite system consisting of dCas9, a gRNA fused to an RNA binding motif...

...modeling, directed evolution, site-saturation mutagenesis, and medium-throughput screening, she increased... plant surfaces. His system of choice is the Arabidopsis thaliana - Pantoea agglomerans interaction. But...

...visualized and results were consistent with copy number variation (CNV) analysis of the U2OS cells.   ...

...controlling for journal. And so, while our quantitative analysis cannot rule out all other explanations, we do ...ssrn.com/author=1232466.     Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT...

...Ecologist     Computer and writing skills Writing a thesis or dissertation demonstrates that you have advanced...what each of these roles involves on a dad-to-day basis. Often, it is hard to know what a job title actually...

... prepared me for what a PI without a personal assistant has to deal with. My inbox currently stands at...advisor Wally Gilbert: “Very interdisciplinary, with emphasis on genetic engineering, sequencing, and translation...

... in protein mutagenesis and cancer immunotherapies. She is currently a Research Assistant in Arlene Sharpe...

...population is specific for your target antibody and may assist in gating your target population (b). There..., so you can use most of your samples for flow analysis rather than for setting up your initial voltages...

...Joung and Jennifer Doudna Labs used targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This... sites. Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9...

...probable mature human miRNAs. Weiss’s collection consists of a library of reporter plasmids, each containing...However, a CRISPR-based tool for in vivo functional analysis of immune cells has been a gap in the field. Arlene...

... day of the interview, dress the part, and be consistent with your personal statement, while you make ...success. Every student should use these guidelines consistently across a number of years as you apply for internships...

...The ITRs base pair with each other to allow DNA synthesis of the complementary strand to yield dsDNA. Unlike...ones listed previously). E3/E1B suppresses both apoptosis and the host immune response to the virus to allow...

...have become a gold standard in gene expression analysis to investigate the effect of regulatory elements...you choose. Many luciferase assays require cell lysis as the most efficient means to disrupt the cell ...

...integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the same sequence...:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP. This visual...

...clarification, meat tendering, and oligosaccharide synthesis. A major incentive to use cold-active psychrophilic...infinity, and beyond! Keeping in spirit with iGEM’s emphasis on holistic, community-centric science, our team...

...selected to participate on this panel, but my consistent writing for the Addgene blog (~1-2 blog posts...social media, but how you use social media to consistently engage with others about your topic(s) of interest...

...emphasizing the importance of critical thinking, persistence, teamwork, and communication. And as a program...breathalyzer to pesticide breakdown, production of fire-resistant housing insulation, and cleaning up salt runoff...

...flies? Sure, the vector will have an antibiotic resistance gene to select for transformed bacteria, but ...Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut. Cell. 137, 1343–55 ...

...this multi-pronged attack. Inflammation and tumorigenesis The conference opened with a very interesting...highlighted the UCSF Immunoprofiler - a human biopsy analysis platform focused on analysing immune measures ...

...can be (part of) figure 1 of your next paper or thesis. This series of posts explains how to critically...Goedhard and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

...Less than 20 ng of total gDNA is sufficient for analysis. We have routinely successfully analyzed gDNA .... 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...

...Institute were instrumental in helping us establish consistent, high-quality protocols for lentiviral production...at Addgene. Our Board helps keep our focus on assisting scientists and accelerating research and discovery...

...journal. An increasing number of funding bodies insist that the research data resulting from their funding...and make up their own minds on whether the data analysis has been of good quality and whether they agree...

...using words for their field or technology in a consistent way. Just creating a lexicon was not enough. ... using the same words with the same meanings consistently, meetings will be shorter and more productive...

...antibiotic in the plate correspond to the antibiotic resistance marker present in your plasmid. You should also...is due to a PCR mistake or a mistake by the DNA analysis software. Now that you've read our plasmid troubleshooting...

...the inter-molecular annealing or second strand synthesis. In contrast, a scAAV vector with half the size...promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001). PubMed PMID: 11509958...

...centromeres. These genomic regions are prone to persistent R-loop formation, presumably due to their abundance...considerations when using these reagents. RNA:DNA hybrids Persistent RNA:DNA hybrids that arise during transcription...

...systematically and logically is as useful for finishing your thesis as it is for helping your team reach an important...thinking, decision making, creativity, or data analysis. Conveniently, these are all skills you are already...

...Agrobacterium (such as Nicotiana benthamiana or Arabidopsis thaliana (Xing et al., 2016)). During Agrobacterium-mediated...Grefen and Blatt, 2012). The 2in1 Plasmid Toolkit consists of 4 plasmid toolkits generated with 4 different...

...bioinformatic tools, data and code sharing, and data analysis and visualization. Not everyone in the group was...Reproducibility Read a blog post on Unique and persistent IDs for improved reproducibility Other resources...

...other genetic disorders, pre-implantation genetic diagnosis (PGD) screens embryos to find those that do not...determine its genotype. However, this detailed analysis is not possible with IVF, as most of the cells...

...technical details of the problem you're solving, but consistency is key. Some namespace collisions you may encounter...treating these pieces of data in ways that are inconsistent with how they are being treated in the lab, ...

...collaboration with the Joung Lab, they used targeted mutagenesis to create HypaCas9 (SpCas9 N692A/M694A/Q695A/...PMC4139937. 3. Anders, Carolin, et al. "Structural basis of PAM-dependent target DNA recognition by the Cas9...

...presence? Learn a key new job skill? Finish your thesis? Knowing your desired outcomes will help you assess...For example Dora Farkas, founder of Finish Your Thesis (who has also blogged for us) offers a free report...

...as in important physiological states such as adipogenesis and macrophage activation. Our mass spectrometry-affinity...coregulators in HeLa cells was the first comprehensive analysis of the interactome of these key regulatory molecules...

...always helpful to remember that if a PI asks for assistance in the grant application process, she has faith...deem your proposal successful. Typical proposals consist of 3 to 5 aims, with secondary milestones to meet...

...All destination vectors contain an ampicillin resistance cassette and an origin of replication, which ...pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion...