We narrowed to 794 results for: Tec

... themselves. All of them have cool new tools, techniques, or information that is now available for other...demethylation. AAV Q&A with Tim Miles: While not technically a research story, I couldn't resist adding in...learning about it (and maybe even picked up a new technique or tool to try in your own work!) If you've got...

...using the streak plate technique. Time for the #BioSci3319 #StreakPlateChallenge part 1. Lab B needs a...Sciences at Missouri University of Science and Technology, has been teaching the microbiology lab every...

...CRISPR continues to grow as the genome editing technique has developed and improved. The CRISPR posts written... for more detailed information about this new technology. Our Plasmids 101 series continues to grab scientists...questions or if you have a topic, researcher, or technology you would like us to feature in the coming year...

...got to learn about intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale...DeNatale and programming with our Chief Architect Pam McA' Nulty (check out our blog post on CRISPR software...presentation on the emerging CRISPR genome editing technology was a rousing success! Many of the students were...

... in skeletal muscle by exercise and endurance, protects the brain from depression. Mice that are genetically...stressors as usual, but somehow their brains were protected. Effects of Activation of the PGC-1a1 Pathway ...levels of kynurenine in the body, the brain is protected from stress-induced biochemical changes that could...

...them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an exciting way of listening to microbes...vesicles) as acoustic biomolecule-based dyes. This technology is widely applicable as bacterial expression ... high spatial resolution are benefits of this technique. It is even possible to multiplex ultrasound dyes...

...blog post, keeping up with the newest CRISPR technologies and their applications can be exhausting. A ...addition to mutations in the spacer itself, will protect the HR template from the Cas9. Again, if it's within...of the Cong et. al (2013) Science paper). If you detect positive HR, isolate single-cell colonies, grow...editing, regulating and targeting genomes. Nature Biotechnology, 32(4), 347–355. https://doi.org/10.1038/nbt...

...perturbations – in the case of screens using CRISPR technology, gRNAs to knockout genes – are essential screening...the validity of your biological model and the technical performance of your assay. But, in many discovery-oriented... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...

...are also the foundation of multiple important technologies, including FRET and optogenetics. Even though...photoconvert GFP from green to RED! This simple technique has been shown to work in plant, Drosophila and...protonated form of GFP is photoconverted, so this technique may not be appropriate for high-pH cellular compartments...

...career goals, and celebrate accomplishments. These techniques are laid out in Vincent et al., and are easily...a recap of accomplishments, from learning new techniques to finally figuring out a tough experiment, sets... in every lab, but the principles behind this technique are beneficial for researchers at all stages. ...

...inner beauty of familiar flowers." This new x-ray technique provides scientist with a new method to explore...by Darwin on his voyage. Previous destructive techniques were incompatible with the need to conserve these...Richards’ work is held by The Museum of Jurassic Technology in LA, where it is currently on display.   Many...

...service. A small subset of AAV plasmids will have technical or legal restrictions that make them ineligible...be reviewed by Addgene for legal approvals and technical compatibility with our production process. This...found in our in-stock preps. If a request has technical considerations that may affect expected titers...

...2015 Find Hot Plasmid Technologies Write about Your Favorite Plasmid Technology Resources on Addgene....it is requested from Addgene many times as the technology disseminates throughout the research community...

...environmental conditions, cells must be able to detect extracellular stimuli. One way they do so is through...pathways.  This dissociation can be measured using a technique known as Bioluminescence Resonance Energy Transfer...effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists and ...

...to learn techniques from each other, access specialized equipment, or ensure that techniques are being...part of your collaboration. Luckily, there are techniques and tools that helped make my experience run ...

...excited about our new partnership with Allele Biotechnology which allows researchers to deposit plasmids...mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited at Addgene. What makes...colleagues from The Scintillon Institute, Allele Biotechnology, Florida State University, Indiana University...

...present in prokaryotes, and some of these proteins protect against invading DNA. In 2014, Swarts et al. characterized...the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub 2016 May 2. PubMed PMID: 27136078. Find...Resources on Addgene.org Check Out Other Argonaute Technologies from the Doudna Lab Browse the CRISPR Collection...

...more standardized methods for evaluating CRISPR technologies, including the ever important on/off-target ... are aimed at improving translation of CRISPR technology, but they would also bring benefits to researchers...pre-clinical research. However, standard terms and techniques are not enough to move the CRISPR field forward...

...with people, nurturing networks, as much as with technical knowledge. I leverage a breadth of knowledge to...I am constantly teaching – often explaining a technical piece of research to others outside that specialty... thinking about, for example, how to use new technologies to reach the right audiences and how open access...

...the less acknowledged perks of scientific and technical training is that these educational paths prepare...encompasses millions of choices including pharma, tech transfer, management consulting, science communication...Small Company Big (e.g. Pharma) Small (e.g. Biotech) Develop expertise areas quickly Jack/Jane of...

...lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org/10.1007/s13205-012-0070...vectors using reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org/10.2144/000112585...4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank C, Aneja MK, Rudolph C (2019...

...Additional Posts on Mol Bio Techniques Find Additional Posts on Plasmid Technologies Resources at Addgene.org...Plasmids in the Repository Learn about New Plasmid Technologies in our Hot Plasmid Articles ...

...how they function. Adapting Toehold Switches to Detect Zika Virus The RNA-based toehold switches described.... These toehold switches can be used to quickly detect the presence of Zika virus. Read this post from...Combinatorial Genetic Screening CombiGEM is a technology that can be used to quickly barcode a set of ...

...many developments in science and the plasmids, technologies, and researchers behind them. As part of our...started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed to provide...will be sure to keep our eyes open for any new technologies that will bring about the next revolution in...

...individuals in their 20’s who’ve built multibillion dollar tech companies using only laptops and some server space...our network.  We started with the idea of a biotechnology investment fund that would apply a percentage... biotechnology...

...type; however, their usage has been limited by technical problems. For instance, it’s much more difficult...proteins and reporters Previous Protein Delivery Techniques: Introduction of macromolecules into cultured...homing an artifact? Lundberg M, Johansson M. Nat Biotechnol. 2001 Aug;19(8):713-4. PubMed.  ...

...Escherichia coli or Saccharomyces cerevisiae with few techniques that are easily transferrable across host species...for rational, straightforward design. Since the technique relies on base pairing, it has promise to be host-independent...engineering, this is such a powerful and flexible technique that it should be on the radar of every molecular...

...AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic inhibition Flp expression...) AAV-hSyn-EGFP Biosensor AAV The ability to detect the neurotransmitters and other bioactive molecules...sensor. This “contrast” variant provides improved detection for tracking large populations of neurons in wide-field...

... for gene editing. They named this new array architecture the DAP array, short for “drive-and-process....payloads. Different from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely...Comparison of previous representative dual-gRNA architectures with DAP array   Qichen Yuan is a PhD...

...institute. A common reason is hands-on training in a technique that only a few labs have expertise in. Visiting...sure you read any relevant literature for the techniques you will be performing to familiarize yourself...help you with your research and teach you new techniques. Show up on time (don’t make your bench mentor...

...turns 10 years old, it’s easy to forget that this technique isn’t limited to neuroscience. In fact, precise... method for the computational problem of edge detection. Tabor et al. subsequently engineered a second...publication at Addgene. A synthetic genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier...

... and open source software, hardware, wetware, technology development, etc). Second, the potential hire...job satisfaction (especially for scientists and technical people) is linked to engagement and access to ...you would like to head for a career in pharma or biotech, this is one case where the topic of your research...

...visible part behind these changes: the backend technology that powers the new website search. Tackling ... resources, blog posts or reagent items.  The technology behind Addgene’s new search  Like millions of...PI labs and institutions. There are many more technical details that make our new search engine unique...

... sharing, there will always be an interest in protecting intellectual property. Figuring out that balance...year and we have a very dominant presence in the biotech sector in both patent law and general life science...Young, LLP, auditing insurance, finance and high-tech industries. He has earned both an MBA in finance...

...acids in the domain). Yan et al. from Arbor Biotechnologies found that RspCas13d accessory protein RspWYL1...functional in multiple species (read Arbor Biotechnologies' press release about their Cas13d deposit here...Addgene. Learn more about research at Arbor Biotechnologies. Additional Resources on the Addgene Blog:...

...fluorescent molecule. A higher brightness increases the detected signal and therefore high brightness is an important...brightness that does not fold well or cannot be detected in a microscopy setup has zero practical brightness...excitation wavelength, available emission filters, and detector sensitivity) and the biological system (temperature...

...Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from Cpf1-mediated... is one of the latest developments in CRISPR technologies.  It’s a simple and effective method with multiple...CRISPR–Cpf1 Asing a Single crRNA Array." Nature Biotechnology 35.1 (2016): 31-34. Pubmed PMID: 27918548. 2...

...heterotrimeric G protein complex that can be used to detect G protein activation via bioluminescence resonance...response in vivo (Paek et al., 2017). In this technique, the receptor fused to APEX2 catalyzes the biotinylation...are then identified with mass spec. Using this technique termed “GPCR-APEX” the Kruse lab was able track...

...gaps and add to them as techniques evolve. Blog posts get out of date as techniques and knowledge changes... useful information on scientific literature, technical skills, and experiences from thousands of laboratories...

... creativity in employing a variety of cloning techniques and troubleshooting methodologies drew me to ...antibodies are commonly used in research laboratories to detect the different subclasses, but there are also secondary...use of subclass specific secondary antibodies to detect multiple targets with different mAbs in the same...