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We narrowed to 15 results for: Tec

Showing: 1 - 15 of 15 results

  1. CRISPR Guide

    Type
    Guide
    ... range of genome engineering techniques . Collectively, these techniques are referred to as CRISPR ( C...molecular detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform... to SHERLOCKv2 to detect multiple targets. For example, Cas12 can be added to detect the presence of infectious...have an interesting adaptive immune response that detects and eliminates foreign DNA, which scientists have...egularly I nterspaced S hort P alindromic R epeat) technologies. Before CRISPR, genome engineering approaches...CRISPR one of the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens...CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids. Acr family...

  2. Antibody Guide

    Type
    Guide
    ...amplification through the indirect detection method. In the direct detection method, the signaling molecule.... Figure 3: A western blot detecting proteins using the indirect detection method, with a secondary conjugated...conjugated, it is often called the detection antibody. If using an indirect detection method, add a secondary antibody...microscopy techniques used, so be sure to read materials specific to your assay. Most IF techniques use a ...terms, summarize antibody production and storage techniques, and explain common applications. Science...secretions (e.g. tears, saliva, mucus) where it protects mucosal membranes Dimer with a valency of 4 IgA1...function is unknown Monomer with a valency of 2 IgE Protects against parasites and is responsible for driving...

  3. Molecular Biology Reference

    Type
    Guide
    ...multiple codons. Plasmids and Recombinant DNA Technology Techniques in chemistry enable isolation and purification...been overcome by the field of recombinant DNA technology, which enables the preparation of more managable...most commonly in the field of recombinant DNA technology have been optimized for their use of studying...methods check out our guide on molecular cloning techniques . Plasmid Elements Plasmids used by scientists...purification) and manipulation. With current cloning technology, it is easy to create and modify plasmids containing...editing is most commonly accomplished using CRISPR technology. CRISPR is composed of a DNA endonuclease and...not only give the scientist with an easy way to detect plasmid-containing bacteria, but also provide those...

  4. Optogenetics Guide

    Type
    Guide
    ... The tools and technologies developed for optogenetics research utilize light to detect, measure, and ... when combined with site specific recombinase technology like Cre-lox, which allows you to express an ...targets into large, optically assembled protein architectures in living mammalian cells. LITE Two-hybrid system... Schnitzer MJ. 2006. Next-generation optical technologies for illuminating genetically targeted brain ...

  5. Science Guides

    Type
    Guide
    ... The tools and technologies developed for optogenetics research utilize light to detect, measure, and ...of guides that describe how popular scientific techniques work Science...interest. Read More Chemogenetics Chemogenetics is a technique that uses genetically engineered receptors to ...

  6. Cloning

    Type
    Guide
    ...Reference Choosing a Molecular Cloning Technique Molecular Cloning Techniques You may also like... Cloning Resources...Addgene's reference guide on molecular cloning techniques, including information on seven commonly used...allow you to do many different molecular biology techniques (such as fusing it with different tags, putting...

  7. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...optical tools developed by researchers are used to detect, measure, and control molecular signals of cells... ddPCR AAV Titration by qPCR Using SYBR Green Technology References Burger, C., Gorbatyuk, O. S., Velardo...which together form the outer capsid shell that protects the viral genome. Helper plasmid E4 in trans Adenoviral...cell to release them. Optogenetics Biological technique to control the activity of cells, often neurons...

  8. Educational Resources

    Type
    Guide
    ...’s repository , and related molecular biology techniques. Topics include: Genome Engineering Fluorescent...Fluorescent Proteins CRISPR Viral Vectors Plasmid Technology Career Readiness Molecular Biology Learn about plasmids...Genetic Code Strain Information Molecular Cloning Techniques Protocols Find written and video protocols for...

  9. Guide to Using Pooled Libraries

    Type
    Guide
    ...so it’s essential that you have access to this technology if you’re planning to conduct a pooled library... reaction. As discussed above, access to this technology is crucial for conducting pooled library screens...

  10. Plan Your Experiment

    Type
    Guide
    ...off-target effects. More information on each of these techniques can be found in our blog post CRISPR 101: Validating...genome editing in vitro and in vivo. Nature Biotechnology . 33 (1), 73–80. PMID: 25357182...

  11. Lentiviral Vector Guide

    Type
    Guide
    ...accessibility (DIVA) identifies alterations in chromatin architecture through large-scale mapping of lentiviral integration...achieves efficient gene delivery in vivo. Nature Biotechnology , 15 (9), 871–875. https://doi.org/10.1038/nbt0997...

  12. Addgene's eBook Collection

    Type
    Guide
    ...nomenclature, and uses to common promoters and cloning techniques! Close About Overlay Select CRISPR 101 January...

  13. Promoters

    Type
    Guide
    ...ranging from the newest breakthroughs in plasmid technologies and research to overviews of molecular biology...

  14. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... A. (2011). Gammaretroviral vectors: Biology, technology and application. Viruses , 3 (6), 677–713. https...

  15. Adenovirus Guide

    Type
    Guide
    ...Ad35 vaccine vectors bypass immunity to Ad5 and protect nonhuman primates against ebolavirus challenge ...
Showing: 1 - 15 of 15 results