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Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post...systems described in companion papers in Science in December were developed as an answer to that question. ... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog Post...They first assessed the activation of different members of the transducerome for both well-studied and ... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...function in these systems. As a part of this effort, members of the Rawlins Lab have compiled a new toolkit ... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post...that allow charged particles to cross the cell membrane when exposed to light. Neuronal firing depends... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog Post...offered by eScience Labs. “We work with faculty members to make sure the courses encompass what they want... -
Oh, The Places You Can Go: Careers in Science Communication - Product Development
TypeBlog Post...coordinator. With the guidance of one of the faculty members, I helped grad students who were teaching for the... -
Hot Plasmids - October 2022
TypeBlog Post...photoactivation of HcKCR1 in mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...(plus bonus podcast with Addgene Advisory Board member Alex Chavez) Day 11: Pooled libraries: Genome-Wide... -
SunTag and Fluorescent Imaging
TypeBlog Post...for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18-fold brighter than... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post...communication through art. Addgene Advisory Board member Siouxsie Wiles gave us a fascinating and fun look... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog Post...does best to identify the work style of each team member) Need challenging work, opportunities to pursue... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...expression system and has its advantages; however, remember that you will need accessory packaging vectors... -
10 Ways to Share Your Science!
TypeBlog Post...right track if they start asking you questions. Remember that people like a good story and science involves... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post... EtOH Polymyxin B polypeptide Alters outer membrane permeability Bactericidal 10-100 ug/mL Tetracycline... -
E11 Bio PRISM Collection
TypeCollection...Cre-Lox Plasmid Collection Content last reviewed: 5 September 2025... -
Institute for Protein Innovation
TypeCollection...pairs bridge the presynaptic and postsynaptic membranes at neuronal synapses. Image credit: IPI Browse... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog Post...sequence was “optimized” so that it more closely resembles that of the host organism, it may express protein... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post...and is used by scientists around the globe. As a member of the Brassicaceae family, Arabidopsis is related... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...Enhanced CRISPR Genome Editing Chris Richardson and members of Jacob Corn’s lab describe how a better understanding... -
A Tour of Addgene's Most Popular Pooled Libraries
TypeBlog Post...broad host range regulatory sequences ready to be assembled with other elements using golden gate cloning.... -
Summer SciComm Series: Master of SciComm
TypeBlog Post...communication is an equally fruitful endeavor for those embarking on the route towards academia. Many of those entering... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...my song about our paper recently published in @nchembio Title: Bye bye L-arabinose drive. Dedicated to... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...CellTag to track the direct reprogramming of mouse embryonic fibroblasts (MEFs) into induced endoderm progenitors... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post...reactive to a certain isotype will bind to all members of that group. If you use two antibodies that are... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...successfully track nuclei in a developing zebrafish embryo over an 80 minute time course. By stitching together... -
Viral Vectors 101: Transductions
TypeBlog Post...specific viruses available to choose from. And remember, we have a selection of ready-made viruses as ... -
Summer SciComm: Science Storytelling
TypeBlog Post...” and that’s a very valid critique! But do you remember earlier when I said that even when we know the... -
The Strength of Story Telling
TypeBlog Post...of Nature magazine. Humans are built to enjoy, remember, and listen to narratives — take advantage of ... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...communication and sharing will only help research advance. Remember: productivity is infectious! Additional Resources... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...play a role in many cell processes involving cell membrane dynamics. When they learned there was a disease... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post... are everything and you want to leave people remembering your potential as an entrepreneur, not your credentials... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post...of interest into the FP-SEC vector using Gibson assembly. This construct will serve as the repair template... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...J. et al. Designing and using RNA scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797... -
Viral Vector Guides and Plasmids
TypeCollection...the necessary components of the viral genome to assemble the viral capsids (packaging and envelope plasmids... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...Kamen's LinkedIn network! The connections between members of her network give rise to a starfish shape. ... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...polling feature or the chat box. One key thing to remember regardless of the type of session you’re presenting... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...shipped out of the Krogan lab at UCSF. Our team members (shout out to Margaret Soucheray, Jeff Guo, Paddy... -
The MTA at Addgene
TypeBlog Post...transferring! Many thanks to our Tech Transfer team members Maura Coughlin, Darcy Morse, and Matt Takvorian... -
DAP arrays for multiplex gene editing
TypeBlog Post...Typically, a single array encoding the tandemly assembled tRNA-gRNAs is placed downstream of a lengthy promoter... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog Post...is a science writer, former scientist, and proud member of Crowdfight. He is currently based in Spain and... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...trainee in your new lab, so make a good impression! Remember that your host is going out of their way to help... -
K. phaffii: Rising to the Occasion
TypeBlog Post...proteins. Peroxisome studies Peroxisomes are small, membrane-bound organelles essential for sequestering certain... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog Post...libraries. Here are the key points you’ll need to remember when undertaking library amplification and sequencing... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog Post...including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from direct editorial work... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...actually he drives better after a few drinks. A family member approached me for advice on her baby’s health but... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog Post...investigating DNA repair pathways. Additonal Corn Lab Members who worked on this publication: References... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...other plasmid-based systems, which will enable the assembly of larger systems. The work of Schmidl et al. ... -
Resume Writing for Non-academic Science Careers
TypeBlog Post...skills, and the job you are applying for. Also remember that a resume is typically no more than 2 pages... -
Advice for Scientists Starting a Lab
TypeBlog Post...scientists to their labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early ...