...Bacterial Expression Systems. Check out our Genome Engineering Overview and our CRISPR guide to find out how...how CRISPR is being used for bacterial genome engineering. To make it easier for you to find plasmids ...

...sciencewithtom.com.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...discoveries from her lab. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     References...

...tools are unresponsive to native ligands and are engineered to respond to small molecules that do not affect...Designer Drugs (DREADDs), like RASSLs, are based on engineered G-protein coupled receptors, but unlike RASSLs...Ligands Muscarinic-receptor based DREADDs were engineered to respond to nM concentrations of clozapine ...Actuator Modules (PSAMs, pronounced SAMs). PSAMs are engineered α7 nicotinic acetylcholine receptor (nAChR) domains...clinically approved drug, the PSAM domain was engineered to respond specifically to the anti-smoking drug...through viral injection or use of genetically engineered mouse models. For viral injection, Adeno-associated...plasmids are available. Additionally, genetically engineered animal models expressing hM3Dq, hM4Di, and rM3D...

...Yeast Prototrophy Kit Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...Vincent's tweets.      Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...Vincent's tweets.     Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...You can try a few different lysing buffers and strengths. If that’s not working, consider using a subcellular...incubation (10–20 minutes) at 70 °C, or a truly lengthy incubation (30–60 minutes) at 37 °C, if you suspect... Different lysing buffer/adjust lysing buffer strength Sonication based lysing protocol Subcellular...

...details. "Understanding this structure may help us engineer around the current limitations of the Cas9 complex...said study author F. Ann Ran, a graduate student in Feng Zhang's lab, in a press release. "In the future,...

...missing out on an opportunity.” - Sophia Cheng, Senior Software Engineer “Searching for a new job can be an ...

...2016 was an exciting year for genome engineering research. A variety of new tools came out including ...here. References 1.Ran, F. Ann, et al. "Genome engineering using the CRISPR-Cas9 system." Nature protocols...

...kinase activity is FRET, but FRET reporters are challenging to design and difficult to multiplex. The Covert...which fluorophores can be multiplexed together. Challenging  to Design: FRET reporter activity depends on...

...for mammalian gene silencing. TALENs - Genome engineering methods improved rapidly in 2011, from zinc finger...labs around the world. Thank you! It's been a challenging and rewarding journey since starting Addgene....

...graduate student Silvana Konermann first entered Feng Zhang’s lab at MIT, the use of customizable DNA-...transcription-activator-like effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann...

...available in 2017 is pmSyn1-EBFP-Cre from Hongkui Zeng’s lab. This AAV has had over 150 orders since coming...tools in your experiments.  Many thanks to Hongkui Zeng’s lab for providing this AAV vector! Additional ...

... about salary, opportunities for advancement, challenges and disappointments. These more personal questions...people honor. Talking with strangers is usually challenging but does get easier with practice. Another perk...

...twitter @aigilson.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

... However these may require restriction fragment length polymorphism analysis, allele-specific PCR, T7EN1...Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania.     References...

...Navigating a fulfilling career in science can be challenging – is advice and guidance from only 2 people enough...doesn’t fish for you) Helps identify a Mentee’s strengths and make the most of them Is not threatened by...

...which helped researchers visualize norepinephrine (Feng et al., 2019). But this first generation of tools...blog post. References and resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou...

.... Read the paper in Cellular and Molecular Bioengineering Find the optogenetic repressor plasmids Cytosine...-ME) is a methylation editing tool from Albert Cheng’s lab that enhances RNA-guided methylcytosine editing...

...development, learning, and behavior poses many challenges in neuroscience with many brain regions, neuronal...enhancing anterograde transport efficiency, and engineering viral capsids that target specific cell types...

...trained to be good at learning new things—this is a strength you can draw on and demonstrate in your application...hiring manager. Learn to confidently describe your strengths in your resume and don't be shy about asking for...

... energy and to choose institutions with allies engaged in training excellence to begin effecting change...in their labs. Dr. Richard Roberts, CSO of New England Biolabs, gave examples of how you don’t have to...

...antibody tentacles may have an impact on this challenge either for magnetic capture or for capture using... a rapid covalent bond to a protein, through engineering a bacterial adhesin. Zakeri et al (Proc Natl ...

... focused on scientific outreach and community engagement. Information about contacting Addgene can be ...office in London, Emma Markham. Emma was born in England but has since studied and worked around the world...

... your experiment is definitely not a fluke.” 3. Engage in various projects and help colleagues with their...society. We need much, much more science and engineering (STEM) expertise in law, business, journalism...

...selection screening. They and their colleagues including Feng Zhang used the GeCKO library to identify genes essential...cycle proteins, and more.  “In the past, genome engineering with CRISPRs was limited to modification of a...

... both knowledgeable in their field and eager to engage with the work of others. Having an environment ...at once. I was able to do something that was challenging while helping us share our resources better with...

...macrophages bind IgG antibodies. The macrophages then engulf and digest the pathogen the antibodies are bound...are intriguing from a therapeutic standpoint. Engineered monoclonal antibodies that can drive specific...

...is more on the horizon. Cas enzymes have been engineered to have many unique capabilities which has advanced...Resources CRISPR History and Development for Genome Engineering    ...

...your primary focus lies within a biological lab. Engaging in such seminars exposes you to discussions beyond...release and mental freedom. Practical approaches Engaging in mindful meditation, taking field trips, and...

...Bacteria: CRISPR Methods for Bacterial Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing...and CRISPR History and Development for Genome Engineering  ...

...for laboratory waste symposium, sponsored by New England Biolabs and Labconscious. I was eager to learn ...Labconscious. If you’re inspired by this post then I challenge you to take to action! These days, it’s important...

...widely available imaging platform: ultrasound. Challenges of in vivo imaging with existing bacterial reporters...genes (ARGs) Acoustic Reporter Genes (ARGs) are engineered gene clusters that encode proteins that form ...

...motivating their doers by ensuring they are as engaged as possible. To learn more about being an effective... and Richard Badham Leadership Insights for Engineers, Scientists and Computer Professionals as Leaders...

... the successes of the team. A strong sense of engagement in the work Behind-the-scenes support from their...identify the work style of each team member) Need challenging work, opportunities to pursue and problems to...

...staining can be a challenge in the best of cases. But it can be particularly challenging when your only ...

...using sgRNAiBAR. Image from Zhu et al., 2019.  Wensheng Wei's lab at Peking University has developed a...recombination sites in the construct to different lengths, the density of cells that are labeled can be altered...

...spring requiring an overnight hike in. Navigating challenges: collecting and processing environmental samples...,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable between 31-100...

... positive plants, but screening these can be challenging and requires specialized equipment. Reporters...Additional resources on the Addgene blog  CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis...

...metagenome datasets from hypersaline samples, were engineered and adapted for RNAi experiments for mammalian...mammalian cell lines. The Turner Lab Human messenger-RBP sgRNA Library targets human mRNA binding proteins with...

...controls available when needed. SnapGene has been engineered to be easy and enjoyable to use. Development ...annotation process. Many of the best ideas come from engaging with users of the software. We welcome suggestions...

...other media outlets (AAAS Mass Media Science & Engineering Fellows Program). Science journalism programs...the Advancement of Science. She is enjoys broad engagement through science communication with a variety ...

...Because mNeonGreen wavelengths lie between the green and yellow fluorescent protein wavelengths, it can be imaged...

...23817127. PubMed Central PMCID: PMC3713494.  Ke, Meng-Tsen, et al. "Super-resolution mapping of neuronal...reports14.11 (2016): 2718-2732. PubMed PMID: 26972009. Ke, Meng-Tsen, and Takeshi Imai. "Optical clearing and index...

...Regardless of excitation wavelength, Keima emits at 620 nm. The ratio of signal strength excited at 586 nm to...

...screening platform, Oliver Griesbeck’s lab set out to engineer a new FP. Their aim - to red-shift the boundaries...expansion of the genome editing toolkit. However, a challenge in optimizing base editing technologies across...

...are single domain antibody fragments typically engineered from heavy-chain only antibodies found in camelids...catalytically inactive dCas9 by fusing it to an engineered peroxidase, (APEX) that labels proximal proteins...

...solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering While the vast majority of CRISPR plasmids in...validation process for CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help...