We narrowed to 17 results for: Eng

...lab procedure for separating DNA by size (e.g., length in base pairs) for visualization and purification...longer ones. Thus, you can determine the approximate length of a DNA fragment by running it on an agarose gel...ladder (a collection of DNA fragments of known lengths). Last Update: Feb. 20, 2018 Protocol Video Watch...concentration of 10 mg/mL) Gel loading buffer, New England Biolabs B7022S Procedure Pouring a Standard 1% ... be purifying the DNA for later use, use long-wavelength UV and expose for as little time as possible ...

...absorb different wavelengths of light to varying degrees and most have a specific wavelength that they maximally...based on its absorbance, you need to know the wavelength of light that your substance maximally absorbs...

... (Gibson et al. , 2009). Regardless of fragment length or end compatibility, multiple overlapping DNA ...fragments at a time. Resources and References New England Biolabs (NEB) (Link opens in a new window) sells... window) . Gibson Assembly® is licensed to New England Biolabs by TelesisBio. Gibson DG, Young L, Chuang...

...used for ligation, use only long-wavelength UV light. Short wavelength UV light will increase the chance...Materials Material Vendor and catalog # AgeI New England Biolabs (NEB) #R0552S EcoRI NEB #R0101S NEB buffer...

...gel electrophoresis, which separates DNA by their length in base pairs. Following electrophoresis, you can...cutting out the bands and you will want to use long-wavelength UV for as short a time as possible to get the...

... DNA polymerase can create overhangs of varying length (typically 10-12 bp) based on a specific sequence...or tag sequences (where appropriate). The primer length is dependent on the T4 Pol "chew back" reaction...

...the amount of DNA used in a ligation based on the length of the DNA to get a proper ratio of 3 available...found on the web. Just enter the concentration, lengths of your insert and vector, and what ratio you want...

...cells. The problem is that the only version of full-length cDNA you can find for YGOI is in a bacterial expression...enzyme buffer (see (Link opens in a new window) New England Biolabs for more information about restriction ...

...should generally have the following properties: Length of 18-24 bases 40-60% G/C content Start and end...

.... to ligate an annealed oligo insert of 50bp in length into a 5kb vector, mix 100ng of the vector with...

...commercially available restriction enzymes, see New England Biolabs' website . Last Upload: May 18, 2018 Protocol...

...extension time can be adjusted according to the length of the target sequence. FAQ How do I design primers...

...cells for 13 days. lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described...

...temperatures, but you may need to increase your primer length and increase the Tm if you are trying to clone ...

...loading control. 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described...

... until fully dissolved. Pro-Tip This step is challenging due to the high viscosity of PEG. Stirring under...

...Quality Control (QC) Pooled libraries can be challenging and expensive to ensure adequate quality, but...