AAV Vector Quality Control: Going the Extra Mile with NGS
Type
Blog Post
...the mixed sample in our pipeline (see Figure 2, sample 2). When we blindly analyzed AAV-68544, we were...look for potential contaminants.
The analysis is a 2-step process using Geneious software:
First, the individual...Léger A (2015) Advanced Characterization of DNA Molecules in rAAV Vector Preparations by Single-stranded Virus...especially important when producing multiple vectors in parallel because the risk of accidental errors increases... be used to identify DNA contaminants in AAV preparations and provide information on these DNA species...pioneering the systematic use of this new QC assay to guarantee that you receive the best AAV vectors for your...Identity of Recombinant Adeno-Associated Viral Preparations from Viral DNA Extracts. Human Gene Therapy ...