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We narrowed to 11 results for: ARA-2

Showing: 1 - 11 of 11 results

  1. Optogenetics Guide

    Type
    Guide
    ...Cryptochrome 2 and CIB1 Blue-light–mediated induction of protein interactions based on Arabidopsis thaliana... OPTOSTIM PHR domain2 of cryptochrome 2 (Cry2) from Arabidopsis thaliana is fused to truncated forms of...tools derived from direct comparative analysis of microbial opsins. Nat Methods. 9(2):159-72. PMID 22179551...influx into the cell when stimulated. Channelrhodopsin-2 (ChR2), the first widely adopted optogenetic tool,...thaliana cryptochrome 2 and CIB1 450 LARIAT Inhibits protein function by reversibly sequestering targets into...customizable TALE DNA-binding domain with cryptochrome 2 protein and its interacting partner CIB1 to control... with single-spike temporal resolution. PLoS One. 2(3):e299. PMID 17375185 Kim B, Lin MZ. 2013. Optobiology...

  2. Adenovirus Guide

    Type
    Guide
    ...use of pAdEasy-2 (Addgene #16401) can increase the capacity of the rAdV vector. pAdEasy-2 does not contain... incorporate additional genomic deletions. Figure 2: First-generation rAdV vectors. Created with BioRender.com...and have to be handled at biosafety level two (BSL-2). They are highly immunogenic and can trigger a strong...era of personalized medicine . Genes & Diseases, 4 (2), 43–63. https://doi.org/10.1016/j.gendis.2017.04.001...adenoviruses using the AdEasy system . Nature Protocols, 2 (5), 1236–1247. https://doi.org/10.1038/nprot.2007.135...Adenoviral vector vaccine platforms in the SARS-CoV-2 pandemic . NPJ Vaccines, 6 (1), 97. https://doi.org...Adenoviral backbone plasmid that lacks E1 and E3. pAdEasy-2 Adenoviral backbone plasmid that lacks E1, E3, and...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...intact. First-generation plasmids include (Figure 2): Transfer plasmid — contains transgene and wild-type...envelope removed Envelope plasmid — contains env Figure 2: First-generation lentiviral plasmids. Created with...plasmid 1 — contains gag and pol Packaging plasmid 2 — contains rev Envelope plasmid — contains env Third-generation...The inside out of lentiviral vectors. Viruses , 3 (2), 132–159. https://doi.org/10.3390/v3020132 PMID: ...Infectivity and Integration Targeting. PLoS ONE , 2 (12), e1340. https://doi.org/10.1371/journal.pone.0001340...features of lentiviral vectors. Human Gene Therapy , 24 (2), 132–142. https://doi.org/10.1089/hum.2012.229 PMID...immunodeficiency virus (FIV). Retroviruses are partially characterized by their ability to integrate their genomes ...

  4. Chemogenetics Guide

    Type
    Guide
    ...muscarinic receptor and has not been used in vivo . Figure 2: DREADDs, their ligands, and signaling properties.... Reference Rq(R165L) Human M3 muscarinic Arrestin-2/-3 CNO* Increase Arrestin translocation Arrestin signalling...hM1Dq hM5Dq Human M3 muscarinic G αq CNO* Increase Ca 2+ Neuronal burst firing Armbruster et al., 2007 rM3D...channels, their effect, and outcome in neurons. Table 2. Descriptions of PSAMs and their activity in neurons...Forbes SL, Liggett SB (2001). Modification of the beta 2-adrenergic receptor to engineer a receptor-effector...C, Ono M, Shimojo M, Zhang M-R, Tomita Y, Nakahara J, Suhara T, Takada M, Higuchi M, Jin J, Roth BL, Minamimoto...available. Browse our collection of Ready-to-use AAV Preparations of Chemogenetic Plasmids ! Table 4. Common promoters...

  5. CRISPR Guide

    Type
    Guide
    ...gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately. They can knockout, ...left free to interact with the target DNA. Figure 2: Overview of the NHEJ repair mechanism Cas9 will only...systems enable researchers to target anywhere from 2–7 genetic loci by cloning multiple gRNAs into a single...your experimental cell population (Figure 8E). In a 2-vector system, you’ll need to either co-infect with...the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar to Cas9 and Cas12...Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...off-target effects by using a single Cas9 nickase and 2 different gRNAs, which bind in close proximity on ...

  6. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...components, see the AAV Plasmid Elements table . Figure 2: Plasmids required for rAAV vector production. Created... pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism...the central nervous system . Molecular Therapy, 10 (2), 302–317. https://doi.org/10.1016/j.ymthe.2004.05.024...the production of AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384...retrograde access to projection neurons . Neuron, 92 (2), 372–382. https://doi.org/10.1016/j.neuron.2016.09.021...were discovered as a contaminant of adenovirus preparations, which is how they got their name, but they ...construction, as it was the first to be well-characterized and extensively studied, and has a broad tissue...

  7. Antibody Guide

    Type
    Guide
    ... unknown Monomer with a valency of 2 IgE Protects against parasites and is responsible for driving allergic...such as anaphylactic shock Monomer with a valency of 2 IgG The most abundant isotype in blood, also found...secondary immune response Monomer with a valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary... ranging from antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include...used for assembling protein nanostructures. Figure 2: An antibody (IgG, far left) compared to alternative... can be used in the lab to quantify, identify, separate, and locate proteins of interest. The protein ...use antibodies to identify denatured proteins separated by size on an SDS-PAGE gel. Though relative quantification...

  8. Plan Your Experiment

    Type
    Guide
    ...components (Figure 2), and the optimal system will likely depend on your cell type. Figure 2: Different delivery...either (1) a lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that...CRISPR-Based genome editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006... Villalta, J. E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C....

  9. Science Guides

    Type
    Guide
    ...specific control of activity. Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic...Pooled Libraries Pooled libraries are single preparations of many different plasmids. Plasmids within ...

  10. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains transgene, sgRNA, or...for different cell types (known as tropism). Figure 2: Gamma-retroviral plasmids. Created with BioRender.com...leukemia virus (FeLV). Retroviruses are partially characterized by their ability to integrate their genomes ... vectors, these required components have been separated into distinct plasmids for safety to reduce the...eliminating the need to deliver these genes on separate plasmids ( in trans ). This method, therefore,...The genes used for viral vector production are separated into three different plasmids containing the transfer... A retrovirus from the Retroviridae family. Characterized by long incubation and the eventual cause of...

  11. Molecular Biology Reference

    Type
    Guide
    ...mrr-hsdRMS-mcrBC) Phi80lacZDeltaM15 Delta-lacX74 recA1 araDelta139 D(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG ...+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) ∆(ara-leu) 7697 araD139 fhuA ∆lacX74 galK16 galE15 e14- Φ80dlacZ...mrr-hsdRMS-mcrBC) Phi80lacZM15 Delta-lacX74 recA1 araD139 Delta(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG ...Invitrogen F– mcrB mrr hsdS20 (rB–, mB–) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20 (StrR ) xyl-5 λ– leu ...indicated, the antibiotic powder can be dissolved in dH 2 0. Addgene recommends making 1000X stock solutions...million DNA fragments, which is far too many to separate from each other for the purposes of isolating ...recombinant DNA technology, which enables the preparation of more managable (i.e., smaller) DNA fragments...
Showing: 1 - 11 of 11 results