We narrowed to 591 results for: ARL
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TypeBlog Post...brain tissue is injected with compounds that intracellularly spread away from the site of application and...transport has been observed for AAV1, -5, -8 (McFarland et al., 2009), and bidirectional transport for...Neuroscience 193, 249–258. PubMed PMID: 21782903. McFarland, N.R., Lee, J.S., Hyman, B.T., and McLean, P.J...
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Top Requested Plasmid of 2017 - pMD2.G
TypeBlog Post...envelope vector pMD2.G This plasmid has been requested nearly 6,000 times (over 800 orders in 2017) and expresses...variety of cells and study their effects. To date, nearly 900 articles cite the use of pMD2.G obtained from... -
Bringing Renewable Energy to Addgene
TypeBlog Post...electricity! A (not so) shocking transition In early July the Town of Watertown announced the creation... electricity will allow us to maintain these particularly energy-intensive systems in a more environmentally... -
New Viral Vectors - Spring 2025
TypeBlog Post...Jordane Dimidschstein New serotype pAAV_hSyn-PdCO-mScarlet-WPRE AAV1 Optogenetics Ofer Yizhar New viral ...pAAV-Ef1a-fDIO hChR2(H134R)-EYFP AAVrg Optogenetics Karl Deisseroth New viral tool AiP13987: pAAV-AiE0452h... -
New Viral Vectors - Summer 2024
TypeBlog Post...service with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New...with multiple serotypes pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAV1, AAV5 Optogenetics Yizhar New viral ... -
Performing In Vivo CRISPR Screens Using the FITS Approach
TypeBlog Post...., 2024), developed and deposited at Addgene by Arlene Sharpe’s lab, enables in vivo screens in naïve ...here! Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and... -
Newly Updated AAV Data Hub!
TypeBlog Post...more. A great submission includes: Images that clearly show viral spread and cellular tropism* (min. 300...serotype An abstract that summarizes your experiment clearly and concisely* *required for a DOI. Reports that... -
Interview with Bart Newland, Addgene’s Newest Board Member
TypeBlog Post...Newland. Newland is General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics...up going straight to law school. This was in the early 80s, and biotech was really just taking off. I found... -
Science Communication for Everyone
TypeBlog Post... of Toronto. The Addictive Brain was founded in early 2018 with the goal of communicating science to non-scientists... eLife ambassador, SfN community leader, and an early research career panelist at BioTechniques. Additional... -
Addgene's Top Blog Posts from 2020
TypeBlog Post...Scientists by Jennifer Tsang These tips were written early on in the pandemic. Later in the year, Joanne Kamens...methods for extracting DNA without a kit too. 1. Early Career Researcher Toolbox: Free Online Molecular... -
Our Most Popular Blog Posts from 2019
TypeBlog Post...mouse, fruit fly, yeast, zebrafish, and worm. 4. Early Career Researcher Toolbox: Free Tools for Making...from the community. Are there any topics you particularly like or maybe something you’d like to see more... -
From our Table to Yours: An Inside Look at Lunch at Addgene
TypeBlog Post...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ...food on special occasions. However, employees regularly bring in baked goods and we have a few unique ... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post... systems, developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment of in vivo...here! Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post...production of deletions by paired DSBs may be particularly useful. The first is the study of non-coding...genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 ... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog Post...researchers who joined a recent Twitter chat for Early Career Researchers. Many of the participants had...reach your goals Listens well and communicates clearly and openly Gives honest feedback without ever being... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog Post...is still important. It needs to express value, clearly communicate exactly what a site offers to their...communicate that sense of community, Blugene will regularly be paired with a variety of science-inspired friends... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...alpha-1a adrenergic receptor; nLightG contains circularly permuted green fluorescent protein (GFP), and...green fluorescent proteins. nLightR did perform similarly to GRABNE, and having both colors allows for increased... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post... spectral region in which mammalian tissues are nearly transparent. As Verkhusha explained it to me, the...tissues 20-fold greater than any fluorescent protein earlier described. “Until our proteins, there were lots... -
Fostering communication in remote collaborations
TypeBlog Post...experiments. Therefore, it’s crucial that everyone meets early on to set expectations for the output that will ... which parts of the project, and, as mentioned earlier, how frequently the entire group will meet. You... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog Post...popular bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe... al., 2019). Type I-F CASTs were found to be particularly useful. But they were barely effective at editing...