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We narrowed to 591 results for: ARL

Showing: 181 - 200 of 591 results
  1. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...brain tissue is injected with compounds that intracellularly spread away from the site of application and...transport has been observed for AAV1, -5, -8 (McFarland et al., 2009), and bidirectional transport for...Neuroscience 193, 249–258. PubMed PMID: 21782903. McFarland, N.R., Lee, J.S., Hyman, B.T., and McLean, P.J...
  2. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...envelope vector pMD2.G This plasmid has been requested nearly 6,000 times (over 800 orders in 2017) and expresses...variety of cells and study their effects. To date, nearly 900 articles cite the use of pMD2.G obtained from...
  3. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ...electricity! A (not so) shocking transition  In early July the Town of Watertown announced the creation... electricity will allow us to maintain these particularly energy-intensive systems in a more environmentally...
  4. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...Jordane Dimidschstein New serotype pAAV_hSyn-PdCO-mScarlet-WPRE AAV1 Optogenetics Ofer Yizhar New viral ...pAAV-Ef1a-fDIO hChR2(H134R)-EYFP AAVrg Optogenetics Karl Deisseroth New viral tool AiP13987: pAAV-AiE0452h...
  5. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...service with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New...with multiple serotypes pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAV1, AAV5 Optogenetics Yizhar New viral ...
  6. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...., 2024), developed and deposited at Addgene by Arlene Sharpe’s lab, enables in vivo screens in naïve ...here! Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and...
  7. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...more. A great submission includes: Images that clearly show viral spread and cellular tropism* (min. 300...serotype An abstract that summarizes your experiment clearly and concisely* *required for a DOI. Reports that...
  8. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ...Newland. Newland is General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics...up going straight to law school. This was in the early 80s, and biotech was really just taking off. I found...
  9. Science Communication for Everyone

    Type
    Blog Post
    ... of Toronto. The Addictive Brain was founded in early 2018 with the goal of communicating science to non-scientists... eLife ambassador, SfN community leader, and an early research career panelist at BioTechniques.     Additional...
  10. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...Scientists by Jennifer Tsang These tips were written early on in the pandemic. Later in the year, Joanne Kamens...methods for extracting DNA without a kit too. 1. Early Career Researcher Toolbox: Free Online Molecular...
  11. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...mouse, fruit fly, yeast, zebrafish, and worm. 4. Early Career Researcher Toolbox: Free Tools for Making...from the community. Are there any topics you particularly like or maybe something you’d like to see more...
  12. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ... systems, developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment of in vivo...here!   Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and...
  13. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...alpha-1a adrenergic receptor; nLightG contains circularly permuted green fluorescent protein (GFP), and...green fluorescent proteins. nLightR did perform similarly to GRABNE, and having both colors allows for increased...
  14. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ... spectral region in which mammalian tissues are nearly transparent. As Verkhusha explained it to me, the...tissues 20-fold greater than any fluorescent protein earlier described. “Until our proteins, there were lots...
  15. Fostering communication in remote collaborations

    Type
    Blog Post
    ...experiments. Therefore, it’s crucial that everyone meets early on to set expectations for the output that will ... which parts of the project, and, as mentioned earlier, how frequently the entire group will meet. You...
  16. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...popular bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe... al., 2019). Type I-F CASTs were found to be particularly useful. But they were barely effective at editing...
Showing: 181 - 200 of 591 results