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We narrowed to 238 results for: Anti-CRISPR

Showing: 181 - 200 of 238 results

  1. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... Addgene Blog Learn How CRISPR Can Be Combined with Phage for Antimicrobial Purposes Use Yeast Two Hybrid...Systems to Probe Protein-Protein Interactions Use CRISPR For Genome-Wide Screens Resources on Addgene.org...characterization, these proteins could function as surrogate antibodies for identifying and characterizing sugars and... good start if you’re using a pET vector) and antibiotic for plasmid selection. Remove air bubbles between...temperature. If you’re lucky enough to have an antibody or other protein on hand that will specifically...

  3. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... first ever CRISPR-based clinical trial in 18 lung cancer patients. This study used CRISPR gene editing...are a few of the reasons antibodies may result in a lack of response. Antibodies targeting the tumor-augmenting...effectively in mice. Chimeric antigen receptors T cells producing chimeric antigen receptors (CARs) on their...acquire genetic alterations and alter cell-surface antigen production to evade the immune system. 3. Escape...sidestep immune surveillance: Suppressing Function Antigen presenting cells (APCs) that would normally alert...regulates the expression of the tolerance-producing antigens enunciated above. Finally, signals released from...therapeutic ability of even these conventional antitumor treatment strategies can be enhanced. Types of...

  4. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...on Addgene.org Find simple lab protocols Browse CRISPR plasmids for isolating a genomic locus Browse FRET... culture, because it is easy to harvest large quantities of cells. However, when working on specific tissues...beads. Nuclei are then incubated with a primary antibody against the protein of interest. The Protein A...Immunoglobulin G (IgG) thus targeting MNase to antibody bound proteins (Figure 1). Importantly, since ...is helpful to incubate with a rabbit secondary antibody when using a non-rabbit primary, prior to the ...

  5. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...chimeric antigen receptor (CAR) therapy. SB is a valuable tool and an alternative to CRISPRs for genome...transposase (as determined by transposition assays of antibiotic-resistance genes conducted in HeLa cells). In...

  6. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...fluorescence, SunTag can also be used to improve CRISPR-based activation of target genes, but we’ll save...GCN4. GCN4 recruits GFP fused to the cognate scFV antibody, which is expressed from a separate plasmid. This...

  7. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink...). Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces...

  8. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...translation in living single cells. However, before CRISPR-Cas9 these techniques were quite tedious and, even...are added to a cellular sample. Both antibodies have a short antibody specific DNA strand added to them....PLA where one antibody was directed toward a protein of interest but the second antibody was directed ...most common approach. With a good and specific antibody and the proper microscope setup we can observe...interactions in situ. In the standard PLA assay, primary antibodies directed towards two endogenous proteins of interests...amplification could only take place if the two antibodies recognized an endogenous protein of interest ...proteins in situ. Now FUNCAT-PLA provides both quantitative and spatio-temporal information about the turnover...

  9. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources on Addgene.org Browse Pooled... from both. These products are then purified, quantified, and sent for sequencing. Analyzing your sequencing...

  10. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the...can be both sorted using FACS and isolated with antibiotic selection. The team tested this system in vitro...the addition of standards, the assay is semi-quantitative and can approximate mRNA concentration within...host-range of the plasmids and suitability of various antibiotic resistance markers, promoters, and inducible ...microbiome responds to infection, dysbiosis, and antibiotic treatment. To facilitate these studies, Pamela...

  11. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...Service Tips for 1st Time CRIPSR User (By a 1st Time CRISPR User) Tips for Titering Your Lentivirus Preps ... (Fig. 1). However, once thawed, there was a substantial reduction (from 7.4 to 5.0) in the pH of samples...that dry ice storage affected the performance of antibodies, because of effects of pH on protein stability...infectivity of the virus. However, there was a substantial reduction in infectivity after 3 weeks of exposure...

  12. All in a Twist: dsRNA

    Type
    Blog Post
    ...021-00430-1 More resources on the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA Interference ...This pathway has been clinically exploited for antiviral purposes similar to that of vaccines. If you know...is certainly a double-edged sword – balancing antiviral benefits with the inflammatory response associated...balancing the amount of siRNA to be delivered or anticipating this response are important considerations when...

  13. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has ...viral prep, treated with the antibiotic, and colony-forming units quantified. While these methods tend to...of a gene carried on the transfer plasmid or quantifying the number of viral copies that have integrated...commercially available p24 ELISA kits that can quantify p24 levels in a matter of hours. A major drawback...particle. Consequently, titers based off of p24 quantification tend to be overestimates since they can include...viral RNA is first converted to cDNA and then quantified using qPCR primers targeting specific viral components... components such as LTRs, gag, WPRE, antibiotic resistance-genes, or the transgene itself. Many researchers...

  14. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Representative Empty Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector ...for RNA-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection, and browse our... Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers hygromycin resistance... to easily identify your protein using an antibody against that epitope. This could allow you... your favorite gene even if you do not have an antibody against it. Another common scenario is fusing...

  15. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ... comes in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit...have gained or lost a specific gene survive (ex: antibiotic resistance marker). In a previous blog post, ... your plasmid backbone, as it often relies on antibiotic resistance. But what about identifying clones...

  16. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...Resources on Addgene.org  Yeast-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids...of glycoproteins for human therapies, such as antibodies, is possible in K. phaffii since it produces ...

  17. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...Lab Management Software for Biology Learn about CRISPR Pooled Libraries Resources at Addgene.org Find...Therapeutics, a biotechnology company that uses neoantigen biology to treat cancer. My partner for the day...

  18. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...interactions with Alzheimer's, I found others using CRISPR to generate new knockout mice, using adenoviral...There were, of course, many companies selling antibodies, microscopes, and dissection tools, but there...

  19. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display... a variety of common plasmid features such as antibiotic resistance genes, selectable markers, promoters...

  20. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There isn’t technically...about and jumping into the discussion! Semantic Scholar Semantic Scholar is an AI-backed literature search...the Allen Institute for Artificial Intelligence. Semantic Scholar analyzes publications and figures using...more relevant to your search terms. Key features Semantic Scholar has several ways to focus your search ...at the top of this filter. A unique feature of Semantic Scholar search results are the topic pages, which...displayed. Saving papers Papers can be saved to a Semantic Scholar library. You can add hashtags to library...
Showing: 181 - 200 of 238 results