We narrowed to 253 results for: CIL

1. Protocol - How to Perform Sequence Analysis

   Type

   Protocol

   ...file) provided by your sequencing company or core facility The corresponding primer sequence used for each...

2. Popular Retroviral Vectors and Their Uses in Scientific Research

   Type

   Blog Post

   ...MuLE (Multiple Lentiviral Expression) system facilitates the simultaneous introduction of multiple genetic...

3. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   ...cells for testing in the lab will be greatly facilitated by CRISPR reagents and other specific gene sequences...

4. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

   Type

   Blog Post

   ...therapeutic methylxanthine drugs like theophylline. To facilitate future work with this goal, the Barrick lab has...

5. Science communication: 9 Strategies to Get Your Foot in the Door

   Type

   Blog Post

   ... the distance learning courses at the National Council for the Training of Journalists (NCTJ) or the Alan...

6. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

   Type

   Blog Post

   ...used to maintain a glycerol stock. Choose from: ampicillin, apramycin, blasticidin, bleocin (zeocin), chloramphenicol...

7. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   ... The team found that "all-in-one" Nanoblades facilitated HDR with either a ssDNA or dsDNA donor template...

8. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   ...compatible inserts. All destination vectors contain an ampicillin resistance cassette and an origin of replication...

9. Hot Plasmids February 2024

   Type

   Blog Post

   ...co-incubate it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral...

10. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    ...improved specificity. AAV vectors are able to facilitate and enhance DNA repair via homology directed ...

11. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    ...portions of the Zika virus genome with Addgene to facilitate rapid sharing of these tools with the Zika virus...

12. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    ... RNA transcript followed by a weakly bound poly-uracil tract as shown in the figure to the right. The ...

13. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    ...methyllysine binding domain causes a structural change (facilitated by the flexible linker) that brings the fluorescent...

14. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    ...should have the skills and techniques needed to facilitate effective teamwork, manage day to day operations...

15. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    ...molecular biology and bioengineering, as it has facilitated the development of a simple and scalable means...

16. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    ...has its drawbacks: it requires a camelid animal facility which can be expensive to gain access to routinely...

17. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    ...proteins that can be easily modified in order to facilitate its entry into the target cells (Beatty et al...

18. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type

    Blog Post

    ...Characterization of the Pink and Blue Pigments of Pocilloporid and Acroporid Corals. The Biological Bulletin...

19. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    ...using much smaller regions of homology, which facilitates easier cloning. An overview of PITCh. ...

20. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    ...ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST= dorsal cortico ...