...distributing lentivirus (with many CRISPR tools included among the preps that are currently available) ... AAV preparations have worked well in my lab, including in applications where we have delivered them in...The blog provides tips for working with viruses including advice on how to titer lentiviral preps, troubleshoot...on each viral prep’s specific material page and includes western blots as well as other expression data...

... browse our entire AAV inventory. Our new AAVs include: Optogenetics AAV Control AAV Biosensor AAV And...H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both...have expanded our calcium sensor collection to include our first Flp-dependent GCaMP, AAV8-105714. Several... for the calcium sensor jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. ...

...span four continents and research diverse topics including microbiology, brain injury, drug discovery, and...PZA-resistance by using different approaches, including CRISPR and recombination-mediated genetic engineering...studied various aspects extreme life on earth including extremophile microorganisms, biomarkers, and recently...fluorescent signals from the sensor, and send a signal including its position in the river back to the user.   ...

...the ins and outs of this classification system, including its history, biology, and usages in the lab. History...peptidoglycan membrane. Common gram positive bacteria include staphylococci and streptococci. Gram negative bacteria... lipid membrane. Common gram negative bacteria include salmonella and E. coli. Because of their thicker... For more specific procedural instructions, including timing, preparing the smear, and mixing the stains...

...biological processes is highly useful in other fields, including synthetic biology. Addgene depositors Christopher...effectors are suboptimal for a number of reasons, including their potential toxicity and cross-reactivity ...level of “output” signal, was only about 10-fold, precluding the study of gene expression at very high or ...they can be used with many types of proteins, including Cas9 and dCas9-transcription factors, for which...

...GCE, each component of the experiment system (including media, cell type, and growth temperature) may ...to use a protocol that you’ll have to rework to include reagents accessible to your lab. The solution GCE...is for recombinant expression of phosphoserine-including proteins in E. coli (Zhu et al., 2022). It uses... While they’ve shortlisted several protocols, including their phosphoserine ncAA protocol, to optimize...

...controls are used in antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry...recombination. Heavy chain constant genes are clustered together downstream of the variable region gene...they are the first constant domain genes in the cluster. However, when B cells switch to expressing a different... genes in the heavy-chain constant domain gene cluster. DNA recombination can delete intervening heavy-chain...

...Cancer Biology, where reported methods may not include how many tests were conducted on how the results...reporting guidelines can also help remind us to include all of the essential details. These items rarely...right before data collection starts, and should include a specific plan for how data will be collected,...and reduced bias in scientific research. These include policies for publishers and funders in the Transparency...

...ways foreign DNA can be introduced into cells including transformation, transduction, conjugation, and...often comes from adjacent lysed bacteria and can include plasmid DNA or fragmented DNA released into the...Addgene. For more information on viral vectors, including transduction download our Viral Vectors 101 eBook...into a eukaryotic cell through non-viral methods including both chemical and physical methods in the lab....

.... In the case of stem cells, these factors may include cell type, the disease being treated, and other...immunological, and environmental factors. How to include sex in experimental design Recognizing the importance...Institutes of Health (NIH) now requires that sex be included as a biological variable in all NIH-funded research...differences in effects on the sexes come from, one clue or potential place to start research is by considering...

...also be designed with other DNA-binding domains, including the commonly used LexA and rTetR systems such ...could be delivered throughout the nervous system, including the motor cortex, cerebellum, and spinal cord....easily adaptable to neuroscience applications, including optogenetics, and they should make it much easier...Other proteins may also be utliized as scaffolds, including the set of red fluorescent proteins. The T-DOGGs...

...fat-soluble hormones, vitamins, and dietary lipids. Included in this family are receptors for endocrine steroids... to treat a multitude of therapeutic indices, including hypertension, cancer, diabetes, cardiovascular...these key regulatory molecules, and has provided clues to their previously uncharacterized cellular roles...interface, expanded numbers of curated molecules, including 400 or so coregulators, and a greatly expanded...

... delivery technologies have been investigated including lentiviruses, adenovirus, and inorganic delivery...additional safety benefits: they lack all viral genes, including those that are responsible for integration into...capable of infecting a wide range of cell types including but not limited to muscle, liver, and brain cells...clinical trials have been directed at diseases including but not limited to hemophilia B, LPL deficiency...

.... This tool-kit includes an array of FPs as well as additional useful tags, including 3xFLAG epitopes,... chromatin. A fluorescent protein can still be included for visualization purposes, but the key component...green. This system can be used in many cell types including endothelial cell-pericyte, neuron-astrocyte, and...

...contains both fragments of split mVenus (aa 155), includes I152L in N-terminal fragment An enhanced recombinant... Dan Mulvihill mVenus Split mVenus (aa 155), includes I152L in N-terminal fragment Constant rate of ...  When designing experiments, it’s important to include proper controls to assess the behavior of the split...Biosensors Collection Split Luciferase systems, including NanoLuc®, DULIP, LuTHy Other split protein tools...

...for affecting change. On Friday, the symposium includes a panel discussion and keynote by former congressman...implementation and compliance for the FLSA changes, including finding money in already tight lab budgets and...their advocacy skills. Friday’s symposium will include a discussion on effective advocacy strategies on... on various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington ...

...unstained flow controls). Spectral flow cytometers include a tool called “autofluorescence extraction” which...various tissue types varies, it is critical to include an unstained sample for each tissue type you are... the expression levels of the markers you are including in your panel. Highly expressed markers should...during unmixing. Additionally, it is critical to include unstained beads or cells within your reference ...

...Over the years, Addgene’s repository has grown to include many CRISPR tools.  Distributing and depositing...this tell us? Well, it’s hard to draw any exact conclusions, but it certainly suggests that Addgene depositors...approximately 104 plasmids shipped per working day, including appropriate MTAs and customs forms. Of course,...repository have been cited a total of 23,006 times, including 14,677 unique citations.  CRISPR is a story that...

...year’s Nobel Prize in Chemistry, he joined an elite club, becoming just the fifth repeat Nobel Prize winner...proposed as a mechanism for increasing diversity and inclusion in STEM disciplines (Bangera & Brownell, 2014;...promote professional success (many of my students include their in-class research experience on their resumes...experiences can make scientific research more inclusive. CBE Life Sciences Education. 13(4):602-606. Brian...

...proteins have replicated many of the properties once exclusive to small-molecules: red-shifted spectra, ion sensitivity... have been developed based off of this concept include: Self-labeling tags (e.g., SNAP-tag, HaloTag, ...innovative labeling strategies, several groups—including mine—have been revisiting the old chemistry of...and contain polar groups (Figure 2b), which can preclude their use inside living cells. We recently discovered...