We narrowed to 83 results for: Clu

...Each system also includes Tn7-like transposase components. Type I (generally) includes tnsA , tnsB , tnsC...applications to increasingly complex functions, including the ability to selectively activate/repress target...carried out by Cas9. Multiplexing applications include editing multiple genes at once; using dual nickases...increased fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) — weaken interactions between the...SpCas9-derived variants with non-NGG PAM sequences include: xCas9 — NG, GAA, and GAT; increased nuclease fidelity...adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9 (from Helicobacter sp. MIT 11-5569 ) and...members inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition, inhibiting...

...specifically designed for use in plants. These resources include tools for research using plant systems such as ...expression to reporter systems. Addgene’s collection includes plasmids containing promoters that drive general...GoldenBraid cloning and multigene engineering, including CRISPR/Cas9 parts. Golden Gate (GoldenBraid) Diego...MoClo Level 0 parts for plant transformation, including regulatory elements, coding sequences, basic CRISPR...Compatible with GreenGate Cloning System (#1000000036). Includes modules for CRISPR/Cas9 genome editing, dexamethasone-inducible...GoldenBraid cloning and multigene engineering, including CRISPR/Cas9 parts. CRISPR Diego Orzaez 1000000159...

... of neurons. Common neurodegenerative diseases include Alzheimer’s, Parkinson’s, ALS, and Huntington’s...degeneration of nerve cells in the brain. Symptoms include changes in behavior and emotion, uncontrolled movements...is the most common cause of dementia. Symptoms include memory loss, deficits in decision-making, and language...Addgene's collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. ...Addgene's collections of fluorescent proteins, including Markers of Subcellular Localization , Optogenetics...window) A collection of neurological mouse models including complex and monogenic diseases. Alzforum (Link...scientific community at low cost, with rapid delivery. Includes antibodies, viral vectors, animal models, purified...

...is all-inclusive of several important steps: MTA facilitation for the transfer plasmid, including legal...all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification, and high-...take approximately 6–8 weeks. Additional steps, including request approval, your institution’s approval ...produce high-quality viral vector preps at Addgene, including purification, titration, and quality control measures...information to help you every step of the way, including ordering, handling materials, using them in the...

...types of luciferase found in Addgene's collection include: Firefly luciferase (Fluc) Renilla luciferase (...to as Luc2. Other luciferases in our collection include the red and green Click-beetle luciferases ( CBRluc...cloning. Use with 174051 Alberto Macho 174051 pGWB-cLUC Firefly Creation of C-terminal Fifrefly luciferase...Multiple Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-...luminescence. Find plasmids with LgBit and SmBit . Cluc-FKBP and FRB-Nluc : Split firefly luciferase reporter...reporter of rapamycin-inducible interaction. nLuc and cLuc : Constructs for split firefly luciferase complementation...fused to mScarlet-I. Find more NanoLuc® tools, including constructs for NanoBRET and NanoBiT assays, in...

... these viral vectors undergo quality control, including AAV titration by ddPCR, in vitro and (when possible...Mar;17(3):463-71. PMID: 19066593 Other citations include: Bogner, et al. 2015. Capsid Mutated Adeno-Associated...;19(2):293-301. PMID: 21045809 Other citations include: Chen, et al. 2014. Reprogramming adipose tissue-derived...Sep;26(3):165-76. PMID: 26390090 Other citations include: Scalabrino, et al. 2015. Intravitreal delivery...90(8):4215-4231. PMID: 26865709 Other citations include: Kanaan, et al. 2017. Rationally Engineered AAV...Oct 19;6:35495. PMID: 27759036 Other citations include: Ling, et al. 2016. High-Efficiency Transduction...

...2) to completely unrelated molecules. Examples include: scFv - Single-chain fragment variables (scFvs)... used to quantify relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate ...isolate specific proteins or protein complexes. Includes: Immunoprecipitation (IP) Chromatin immunoprecipitation...Visualization - used to visualize protein location. Includes: Immunofluorescence (IF) Immunohistochemistry ...identify, analyze, capture, and sort single cells. Includes: Flow cytometry Read on to learn more about the...the applications common to each category, including an outline of the basic steps needed for each application...run. The standard curve should contain a range inclusive of all expected protein concentrates in the sample...

...inactivation, NHEJ, and HDR. Includes a wide range of reference genomes, including animals, plants, bacteria...cytosine (CBE) base editors. Includes a wide range of reference genomes, including animals, plants, bacteria...curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community ...common to a set of similar sequences. Organisms include human, mouse, rat, chicken, frog, zebrafish, fly...

...and pooled libraries. Viral preparation options include lentivirus and AAV. ... and widely cited in publications. All pricing includes DNA amplification, viral vector production, purification...community on various aspects of viral vectors, including troubleshooting advice and explanations of viral...DNA Each viral vector service request will also include virus-associated DNA, which is a sample of the ...the Material Transfer Agreement (MTA) and its inclusion is what gives you permission to use the plasmid...

...better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen, energy levels, and...processes. mTORC1 also blocks catabolic processes including autophagy and lysosome biogenesis. mTORC2 does...nutrients, but it is activated by growth factors, including insulin. mTORC2 influences cell survival and cytoskeletal...correlates with poor prognosis in many cancers, including breast, colon, ovarian, and liver cancers. mTORC1...for uncontrolled cell growth and proliferation, including an abundant supply of macromolecules and energy...

...that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins...collections or kits are described below and not included in this table. For a more complete list of plasmids...CRISPR technology. Addgene's worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones,...plasmids used to generate transgenic nematodes, including C. elegans and C. briggsae. Other related miniMos...Lab. A set of vectors for C. elegans research, including lacZ and GFP fusion vectors. C. elegans optimized...

...expression in mammalian systems including hard-to-transfect cell lines. Features include (i) Esp3I (BsmBI) restriction...mRNAs. Both 5’ and 3’ Xenopus β-globin UTRs are included in the vector to enhance expression of the message...Golden Gate protocol. Both plasmids 47388 and 47389 include a Flag epitope tag and an SV40 NLS at the N terminus...mammalian cells. In addition, a T7 promoter was included for generating TALEN mRNAs through in vitro transcription...

...cerevisiae cpFRET Kit includes plasmids to construct custom biosensors ScEnSor Kit includes fluorescent reporters...compartments or organelles. Addgene’s database includes many fluorescent biosensors that signal the presence...processes. Note that many of the articles linked below include variants with different binding affinities or negative-control...indicators for optical imaging (GECO1, GECO1.2), including green, blue, and red indicators An Expanded Palette...Return to top Other Reporters ONE-GO Biosensors Kit includes BRET-based biosensors of G proteins activated ...G-protein-coupled receptors (GPCRs) Yeast GPCR-sensor Toolkit includes plasmids for constructing highly-tunable GPCR-...intracellular environment of S. cerevisiae MOSAIC Kit includes 35 plasmids expressing a variety of fluorescent...

... protein contains several functional domains, including: the N - t erminal D omain (NTD) the M ethyl B...omain (CTD) The most common missense mutations cluster in the MBD and NID demonstrating the importance...in a new window) JAX Mice for mouse models that include MECP2 null alleles or common mutations that recapitulate...window) Coriell Institute - cell line repository, including patient-derived iPSCs (Link opens in a new window...BrainSpan Atlas of the Developing Human Brain - includes developmental transcriptome information, prenatal...

...plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral expression ... approaches combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral expression ... define cellular sub-populations of interest, including dual-transgenic recombinase-expressing mouse lines...the INTRSECT configuration have been developed, including additional fluorophores, excitatory and inhibitory...

...that you want to express a gene; however, we have included a function section for if you want to stably express... pCS for a variety of systems, including Xenopus HA Epitope tag pcDNA3.1-HA - Mammalian...Browse RNAi empty vectors for cloning in shRNAs, including plasmids for constitutive, conditional (Cre-lox...Facilitate the capture and expression of bacterial gene clusters S. cerevisiae plasmids for multiple marker selection...

...For each antibody, we provide important details including the protein and plasmid sequence, the antigen ...Applications section of our product pages. We also include negative results when an antibody’s use is not ...larger Antibody Plasmid Collection , which also includes other types of affinity reagents such as scFvs...

...promoter. For information about each catalog item, including the titer and quality control, visit the material... to Cart *Each viral service request will also include virus associated DNA (vdna), which is a sample ...

...expanded the CRISPR toolbox to include many different types of edits, including point mutations, base changes...Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning...VP64) Many different activator systems exist, including the multi-plasmid SAM system Activate Design and...

... related to cloning and genomic modification, including shuttle, integration, reporter, and tagging vectors... occurring and synthetic regulatory elements, including promoters, terminators, repressors, activators...Addgene's collection of engineered bacterial strains, including plasmid-strain functional pairs. Browse Plasmids...