We narrowed to 445 results for: DUR

...elongation factor that accepts Sep-charged tRNAs during translation. Many ‘flavors’ of Sep-OTS plasmids...pSer-containing GFP that can be used as a positive control during optimization experiments. This version of GFP will...

...constraints given. And there were many constraints during the COVID-19 pandemic. The Pandemic Pivot Back...projects lined up, some that started production during the middle of lockdown, and some that will spin...

...standard protocol, but the primary antibody is omitted during the incubation step. Samples are incubated with...controls address the spectral overlap that occurs during multiplexing. Compensation controls are single ...

...doing an experiment wrong. However, even simple procedures like pouring a plate or running a gel have intricacies... retain searchable, thorough notes on how lab procedures were actually performed, potentially curtailing...

...2025). Nerve-to-cancer transfer of mitochondria during cancer metastasis. Nature, 644 252–262. https:/...progression in cancer models. A) Experimental procedure for xenograft experiments. B) Constructs for tet-inducible...

.... Leaving the membrane overnight in the freezer during one of these steps is also a convenient stopping...Szymanski lab (though she’s tagged along with the lab during its recent move to the University of Georgia). ...

...to transfect, select, and isolate clones. This procedure is cumbersome and inefficient, as it relies on...selection cassette can be incorporated, and used during the differentiation process. Moreover, a splinkerette-PCR...

... intimidating use of discipline or competence procedures Threats to personal standing – including undermining...You are not allowed to speak to lab members alone during the interview period. People seem to be hiding ...

...recombineering substrates to avoid errors introduced during PCR. ssDNA Substrate ssDNA substrates can be generated...MMR’s job is to correct DNA mismatches that occur during DNA replication. There are two ways to avoid activating...

...questions in your own lab. 2. Seek multiple mentors during your postdoc At the end of grad school, you searched...own experience, great mentors often can be found during conversations with senior investigators. Discussions...

...Photostability, the ability to retain fluorescent intensity during exposure to light, is another factor to consider...facilitate clear differentiation between multiple dyes during analysis. Image resolution is impacted by several...

...interest in bacterial and archaeal immune systems. During this time, scientists discovered many related immune...(2015). Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity. Cell, 161(5), 1164...

... visualizing structural changes in the ribosome during protein translation (Ermolenko et al., 2007). All...amber i.e. UAG) engineered into a desired site. During translation, the novel tRNA/unnatural amino acid...

...There are a few different issues that can arise during the plasmid purification process. One is the possibility...from bottles and the overall weight of the package during shipping. Of the kits reviewed here, NEB’s Monarch...

...of the unedited strand Favor the edited strand during mismatch repair   ✓   ✓ ~ ~ ~ ...Yang, W., Leith, A., Daza, R. M., Martin, B., & Shendure, J. (2022). Precise genomic deletions using paired...

...to improve targeting specificity or reduce the duration of Cas9 activity.  The field of optogenetics is... fold induction at 31X. By using a slit pattern during blue light exposure (470nm), the researchers showed...

...pipetting! We’ll release a new tip every two weeks during the school year. Our first quick tip, “Your Bench...

...finding a good name for Blugene’s virus friend.  During the month of January, we’ll be taking name suggestions...

...collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words of welcome...

... we'll be giving away fun Addgene swag for the duration of the conference (be warned, the hand sanitizer...