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  1. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...in-vitro assays: Koch, Daniel, et al. "A pull-down procedure for the identification of unknown GEFs for small... can lead to surprisingly large fitness effects during adaptive evolution of antibiotic resistance." Proceedings...

  2. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...elongation factor that accepts Sep-charged tRNAs during translation. Many ‘flavors’ of Sep-OTS plasmids...pSer-containing GFP that can be used as a positive control during optimization experiments. This version of GFP will...

  3. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ... intimidating use of discipline or competence procedures Threats to personal standing – including undermining...You are not allowed to speak to lab members alone during the interview period. People seem to be hiding ...

  5. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    .... Leaving the membrane overnight in the freezer during one of these steps is also a convenient stopping...Szymanski lab (though she’s tagged along with the lab during its recent move to the University of Georgia). ...

  6. A Control for All Seasons

    Type
    Blog Post
    ...standard protocol, but the primary antibody is omitted during the incubation step. Samples are incubated with...controls address the spectral overlap that occurs during multiplexing. Compensation controls are single ...

  7. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...to transfect, select, and isolate clones. This procedure is cumbersome and inefficient, as it relies on...selection cassette can be incorporated, and used during the differentiation process. Moreover, a splinkerette-PCR...

  8. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...questions in your own lab. 2. Seek multiple mentors during your postdoc At the end of grad school, you searched...own experience, great mentors often can be found during conversations with senior investigators. Discussions...

  10. Typing CRISPR Systems

    Type
    Blog Post
    ...interest in bacterial and archaeal immune systems. During this time, scientists discovered many related immune...(2015). Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity. Cell, 161(5), 1164...

  12. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... visualizing structural changes in the ribosome during protein translation (Ermolenko et al., 2007). All...amber i.e. UAG) engineered into a desired site. During translation, the novel tRNA/unnatural amino acid...

  15. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ...pipetting! We’ll release a new tip every two weeks during the school year. Our first quick tip, “Your Bench...

  17. Addgene's 20th Anniversary Party

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    Blog Post
    ...collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words of welcome...

  18. Addgene at SfN23!

    Type
    Blog Post
    ... we'll be giving away fun Addgene swag for the duration of the conference (be warned, the hand sanitizer...
Showing: 181 - 200 of 441 results