...bacteria, which can uptake plasmids and amplify them during cell division. Because bacteria are easy to grow...Specifically, A pairs with T and C pairs with G. During replication, DNA unwinds and the DNA polymerase...utilizes modified fluorescently tagged nucleotides. During illumina NGS, a long piece of DNA is first fragmented...Addgene all incoming plasmids are sequenced with NGS during our quality control process . NGS allows us to ...

...differ profoundly from viral particles produced during a lytic cycle. rAAV episomes can develop chromatin-like...host cell. In contrast, viral particles produced during a lytic cycle are quickly released through cell...

...are both blunt-ended, can then be fused together during a ligation reaction with T4 DNA ligase. Restriction...easy to add restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested...

...modulating the cell cycle can help as HDR takes place during the S and G2 phases. Chemically or genetically ...cytidine to uridine, which is read as thymidine during subsequent DNA repair, creating a C to T change...CRISPR Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

...functions. Example schematic of an optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed...outside of the brain, rendering the experimental procedure less invasive. Different activation wavelengths...

...infection of 293/911 cells throughout virus production. During experiments, GFP can be used to sort cells infected...

...because they are only able to enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses...

...sites are called off-targets and should be examined during gRNA design. In general, off-target sites are not...

...produce full length virus from the transfer vector during the viral production stage. Many of the lentiviral...

...Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM,...

...valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary immune response; found mostly in blood...