We narrowed to 10 results for: DUR

...bacteria, which take up plasmids and amplify them during cell division. Because bacteria are easy to grow...advantage of the process of DNA replication in vitro. During replication, the DNA helix is unwound by a helicase...incorporation of modified, fluorescently-tagged bases during in vitro DNA replication in addition to the normal...utilizes modified, fluorescently-tagged nucleotides. During Illumina NGS, a long piece of DNA is fragmented...Addgene, all incoming plasmids are sequenced with NGS during our quality control process . NGS allows us to ...

...or which are both blunt-ended, can then be fused during a ligation reaction with T4 DNA ligase. Restriction...easy to add restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested...cloning is a recombination-based cloning method. During Gateway, moving a piece of DNA from one plasmid...

...modulating the cell cycle can help as HDR takes place during the S and G2 phases. Chemically or genetically ...cytidine to uridine, which is read as thymidine during subsequent DNA repair, creating a C to T change...CRISPR Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

...Optogenetics Plasmid Collection . An optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed...outside of the brain, rendering the experimental procedure less invasive. Different activation wavelengths...

...vector is determined by the packaging plasmid used during production, as it carries the Cap region responsible...adeno-associated virus (AAV) integration sites in AAV virions during AAV wild-type and recombinant AAV vector production...

...polymerase. Figure 1: Simplified promoter region during transcription. Created with BioRender.com. DNA ...

...Snowball, A., Knauss, S., von Schimmelmann, M., During, M. J., Lignani, G., Schorge, S., Young, D., Kullmann...

...HER911 cells throughout viral vector production. During experiments, GFP can be used to sort cells infected...

.../10.1128/jvi.72.11.8463-8471.1998 PMID: 9765382 Durand, S., & Cimarelli, A. (2011). The inside out of ...

...valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary immune response; found mostly in blood...