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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 9 results for: DUR

Showing: 1 - 9 of 9 results

  1. Molecular Biology Reference

    Type
    Guide
    ...bacteria, which can uptake plasmids and amplify them during cell division. Because bacteria are easy to grow...Specifically, A pairs with T and C pairs with G. During replication, DNA unwinds and the DNA polymerase...utilizes modified fluorescently tagged nucleotides. During illumina NGS, a long piece of DNA is first fragmented...Addgene all incoming plasmids are sequenced with NGS during our quality control process . NGS allows us to ...

  2. CRISPR Guide

    Type
    Guide
    ...modulating the cell cycle can help as HDR takes place during the S and G2 phases. Chemically or genetically ...cytidine to uridine, which is read as thymidine during subsequent DNA repair, creating a C to T change...CRISPR Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

  3. Cloning

    Type
    Guide
    ...are both blunt-ended, can then be fused together during a ligation reaction with T4 DNA ligase. Restriction...easy to add restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested...

  4. Optogenetics Guide

    Type
    Guide
    ...functions. Example schematic of an optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed...outside of the brain, rendering the experimental procedure less invasive. Different activation wavelengths...

  5. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...vector is determined by the packaging plasmid used during production, as it carries the Cap region responsible...adeno-associated virus (AAV) integration sites in AAV virions during AAV wild-type and recombinant AAV vector production...

  6. Chemogenetics Guide

    Type
    Guide
    ...Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM,...

  7. Adenovirus Guide

    Type
    Guide
    ...HER911 cells throughout viral vector production. During experiments, GFP can be used to sort cells infected...

  8. Lentiviral Vector Guide

    Type
    Guide
    .../10.1128/jvi.72.11.8463-8471.1998 PMID: 9765382 Durand, S., & Cimarelli, A. (2011). The inside out of ...

  9. Antibody Guide

    Type
    Guide
    ...valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary immune response; found mostly in blood...
Showing: 1 - 9 of 9 results