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We narrowed to 753 results for: ELP

Showing: 181 - 200 of 753 results

  1. Advice for Choosing a Research Project

    Type
    Blog Post
    ...some important factors to consider and summarize helpful advice from the successful PIs who were previously...assistance from people who have the required expertise to help move your project forward? Most projects typically...selective, and talking to peers and mentors will help elucidate which project is right for you.   Acknowledgments...

  2. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...plasmid. One of the most frequent problems that I help troubleshoot involves viral vectors and plasmids...incubation temperature to grow bacteria more slowly helps reduce the risk of recombination. Look for the “...Tips for Troubleshooting Viral Transductions will help with the next steps.   References and resources...

  3. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...library-specific identifiers act like a timestamp and help demultiplex the scRNA-seq data and build multi-generational...identified a novel gene, Mettl7a1, whose expression helped coax cells towards successful iEP reprogramming... generating iPSCs Learn how fluorescent proteins help researchers study single cell dynamics in model ...

  4. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... structural and packaging genes, the adenoviral helper plasmid which contains the proteins needed for ...can be organism or tissue type-specific, which can help you restrict the expression of your transgene to...transcribed which aids in nuclear export of mRNA and helps increase the expression of your transgene. The WPRE...

  5. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...secondary, then the following three strategies might help!     Figure 1: Species on species staining...tissue processing and sample preparation can also help reduce the levels of endogenous IgGs in your samples...non-specific staining! Hopefully, these solutions will help get your experiments back on track.  One final note...

  6. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...some of the big problems that optogenetic tools can help us to address? Boyden: There are two concrete areas...biggest ones. And as a third example, it would be helpful to have molecules with better two-photon properties... we've hosted hundreds of visitors in our lab to help them understand their specific problem. Every species...

  7. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...engagement. I figured, an engaging card game could help me get the engagement I wanted. I therefore started...your projects with us! This exchange of ideas will help improve life science and biomedical education for...teaching developmental biology Learn how BioBuilder can help teach students about Synthetic Biology Find Arabidopsis...

  8. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...working with cell culture and who was extremely helpful since she was familiar with library generation.... learn from this workshop? These questions will help me avoid being overwhelmed by all the new information...same if you ever go to a workshop because it will help you think about the most useful pieces of information...

  9. The MTA at Addgene

    Type
    Blog Post
    ...changed a lot over the last twenty years! This has helped us develop a fast, agile approach, which we’ve ...process, you can check out our Tech Transfer Webpage, help center articles, and our MTA YouTube video for more...Coughlin, Darcy Morse, and Matt Takvorian for their help with this post.   ...

  10. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... academic research community. At Addgene, we’ve helped distribute many of these technologies in plasmid...target cells, and, finally, conduct the screen. To help alleviate some of this labor for our users, we now...services. Please send any questions or comments to help@addgene.org. Finally we’d like to thank all those...

  12. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ... are fun, but getting buy-in when delegating can help ensure successful results.  Can you provide the...minutes and plan changes. Written documentation helps make sure everyone is on the same page. Effective... Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete Goals ...

  13. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...experiments. This helped reinforce our goals of making more AAVs and educational content to help researchers... to us, request that we start making the AAV, or help us produce new educational content on the blog. ...

  14. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...ll flood out other signals. But PE can be very helpful if you're trying to find a relatively rare protein...antibodies can get a bit complicated, but hopefully this helps you successfully select - and keep! - antibodies... January 31, 2022, from https://flowcore.syr.edu/help/spectral-overlap/   More resources on Addgene’...

  15. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...cold temperature locales and psychrophilic enzymes help them survive under severely cold conditions. To ...any food processing industry as it can potentially help minimize the incidental losses like spoilage and... and the myriad facilities offered by them which help foster a more productive and collaborative environment...

  16. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...application, our Viruses as Biological Tools blog can help you learn about the different types of viruses and...  Clinical applications Viruses in vaccines helped pave the way for their use in other clinical applications...could further your efforts. And Addgene is here to help! We offer a variety of viral vectors, viral services...

  17. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...summarizing the conclusions of your paper. This will help the editor handling your manuscript decide on whether...English speakers) to proofread your manuscript and help you correct any language- and spelling mistakes....Arguing will make you look unprofessional and doesn’t help your chances of getting your manuscripts published...

  18. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...nifty technique, but what kinds of questions can it help answer? Ramanathan et al. outline three applications...breast cancer. Infectious Disease. RaPID can also help identify which host proteins interact with viral...interactions. Do you have a question that RaPID could help answer? The RaPID plasmids are available from Addgene...

  20. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...obvious, in that a lot of science communication is helping people understand new concepts. You hopefully are... your audience knows, but the following tips can help:  If there’s a teacher, mentor, or other educator...target audience. Thinking about cognitive load will help you understand why you’re not communicating as clearly...
Showing: 181 - 200 of 753 results