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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 25 results for: ELP

Showing: 1 - 20 of 25 results

  1. AAV Production in HEK293 Cells

    Type
    Protocol
    ... must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid expressing your gene of...cells to detach. Gently tap the sides of the CS2 to help detach the cells, add 200 mL of DMEM complete media...μL) Volume of DNA (μL) RepCap 7,265 1.00 727.6 pHelper 11,854 1.00 1,185 Transfer Plasmid 5,842 1.00 584.2...Volume Needed: 582.1 μg / 1.0 μg/μL = 582.1 μL pHelper: 0.08 μg/bp × 11,854 bp = 949.8 μg Volume Needed...carefully tilting the CS5 back and forth. Pro-Tip To help distribute the media amongst the five layers, tilt...

  2. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...agarose gel will help resolve smaller bands from each other, and a lower percentage gel will help separate larger...Placing saran wrap over the top of the flask can help with this, but is not necessary if you pay close...two purposes: 1) it provides a visible dye that helps with gel loading and allows you to gauge how far...

  3. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...agarose gel will help resolve smaller bands from each other, and a lower percentage gel will help separate larger...purify DNA from an agarose gel, including tips to help improve the resolution and separation of bands. ...

  4. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...primers typically anneal to the backbone and can help you verify the ends of the insert. Longer genes ...about the accuracy of your plasmid, please email help@addgene.org and include: The sequencing result The...If the mutation is not an artifact, please email help@addgene.org with the sequence, trace file, and primer...

  5. Weighing Reagents Protocol

    Type
    Protocol
    ...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction For many...cross-contamination with other substances. They also help you transfer the material to your tube or container...then place it unfolded onto the balance. This will help you remove it from the scale later on as otherwise...

  6. Water Bath Protocol

    Type
    Protocol
    ...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction A water...water bath is a piece of laboratory equipment that helps bring your materials to a particular temperature...and maintain the desired temperature. This also helps the water bath heat up quicker. Pro-Tip Set up the...

  7. Video Library

    Type
    Protocol
    ...Protocols page . Please feel free to email us at help@addgene.org with any questions. Protocols Video ...procedures. How-To Videos Screencapture walk-throughs to help guide you through Addgene's online resources. Career...frequently asked questions about the MTA process Help Center How to Deposit Your guide to depositing plasmids...

  8. Pipetting Protocol

    Type
    Protocol
    ...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction The pipette...differently on each type of pipette. This section will help you understand how to read each display to dispense...action between the container and the liquid will help draw the liquid out of the pipette tip). Then, slowly...

  9. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...OR on dry ice for 5 min. Note: This freezing may help the DNA to precipitate. Centrifuge solution at high...pellet by adding 500 µL cold 70% ethanol. Note: This helps to remove excess salt from the DNA pellet. Centrifuge...supernatant after step 6 and before step 7. This will help to remove proteins and other contaminants from the...

  11. Using a Light Microscope Protocol

    Type
    Protocol
    ...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction Microscopes... from the light source through a condenser that helps concentrate the light onto the sample, and then ...

  12. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...not cut elsewhere on the plasmid. Bonus: It is helpful to choose restriction enzymes that can both function...good idea to run an uncut sample of each vector to help with troubleshooting if your digests don’t look ...

  13. Kit Free RNA Extraction

    Type
    Protocol
    ...lysate. Pro-Tip Having your samples spin at 4°C helps reduce RNA degradation. If you don’t have access...

  16. Centrifugation

    Type
    Protocol
    ...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction A centrifuge...
Showing: 1 - 20 of 25 results