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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 753 results for: ELP

Showing: 1 - 20 of 753 results

  1. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...science.  ComSciCon's events have 3 main goals: To help graduate students in all STEM fields become excellent...become ambassadors for their respective fields. To help attendees launch new organizations that will extend...

  3. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...research. If you’re looking for resources that will help you study how chromatin effects your favorite biological...there’s a reasonable good chance that Addgene can help. Though it would be difficult to consolidate every...

  4. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...from us.   Try out the Help Center! The Help Center can be found at help.addgene.org, and the widget is on...message was received is a nice touch, it doesn’t help much if the actual response takes 3 days and still..., as this is vital to ensuring that our services help accelerate your research. We know you don’t have...is why we have a dedicated support team that can help you through any search, ordering, technical, or ...make a phone call or write out an email. You need help right at the moment you’re looking for it, or you...idea here is to build features that allow users to help themselves, saving time and effort for everyone ...use to get support for our resources with our new Help Center! Created using the same reliable Zendesk ...

  9. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...Electrophoresis. 2010 Jul;31(14):2436-41. doi: 10.1002/elps.201000061. PMID: 20589859. Levene SD. Analysis of... on a gel, one of the other methods below should help in multimer identification.   Fig. 2: Gel...such as 30°C or optimizing the incubation time can help maintain cultures in log phase growth. In a study...one of the detection methods described above can help ensure the isolation of the monomeric version of...Let us know in the comments below or contact us at help@addgene.org. Resources and References References...

  11. AAV Production in HEK293 Cells

    Type
    Protocol
    ... must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid expressing your gene of...cells to detach. Gently tap the sides of the CS2 to help detach the cells, add 200 mL of DMEM complete media...μL) Volume of DNA (μL) RepCap 7,265 1.00 727.6 pHelper 11,854 1.00 1,185 Transfer Plasmid 5,842 1.00 584.2...Volume Needed: 582.1 μg / 1.0 μg/μL = 582.1 μL pHelper: 0.08 μg/bp × 11,854 bp = 949.8 μg Volume Needed...carefully tilting the CS5 back and forth. Pro-Tip To help distribute the media amongst the five layers, tilt...

  12. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...agarose gel will help resolve smaller bands from each other, and a lower percentage gel will help separate larger...Placing saran wrap over the top of the flask can help with this, but is not necessary if you pay close...two purposes: 1) it provides a visible dye that helps with gel loading and allows you to gauge how far...

  13. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...agarose gel will help resolve smaller bands from each other, and a lower percentage gel will help separate larger...purify DNA from an agarose gel, including tips to help improve the resolution and separation of bands. ...

  14. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...primers typically anneal to the backbone and can help you verify the ends of the insert. Longer genes ...about the accuracy of your plasmid, please email help@addgene.org and include: The sequencing result The...If the mutation is not an artifact, please email help@addgene.org with the sequence, trace file, and primer...

  15. Weighing Reagents Protocol

    Type
    Protocol
    ...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction For many...cross-contamination with other substances. They also help you transfer the material to your tube or container...then place it unfolded onto the balance. This will help you remove it from the scale later on as otherwise...

  16. Water Bath Protocol

    Type
    Protocol
    ...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction A water...water bath is a piece of laboratory equipment that helps bring your materials to a particular temperature...and maintain the desired temperature. This also helps the water bath heat up quicker. Pro-Tip Set up the...

  17. Video Library

    Type
    Protocol
    ...Protocols page . Please feel free to email us at help@addgene.org with any questions. Protocols Video ...procedures. How-To Videos Screencapture walk-throughs to help guide you through Addgene's online resources. Career...frequently asked questions about the MTA process Help Center How to Deposit Your guide to depositing plasmids...

  18. Pipetting Protocol

    Type
    Protocol
    ...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction The pipette...differently on each type of pipette. This section will help you understand how to read each display to dispense...action between the container and the liquid will help draw the liquid out of the pipette tip). Then, slowly...

  19. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...OR on dry ice for 5 min. Note: This freezing may help the DNA to precipitate. Centrifuge solution at high...pellet by adding 500 µL cold 70% ethanol. Note: This helps to remove excess salt from the DNA pellet. Centrifuge...supernatant after step 6 and before step 7. This will help to remove proteins and other contaminants from the...
Showing: 1 - 20 of 753 results