...viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV-CAG-emiRFP670...help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep...

...possible without the scientists who deposited plasmids to the repository and for the scientific sharing community...the infographic below for stats on our plasmid deposits and requests). Over the past month, we’ve enjoyed...

... Bank (DSHB), directed by David R. Soll, is a repository for monoclonal antibodies, distributing over ...against new cancer biomarkers. We believe that repositories, such as Addgene and the DSHB, play an important... to ensuring affordable access to materials, repositories provide standardized quality-control and technical...

... own the material in our repository. We distribute it on behalf of depositing institutions. Addgene cannot...Help Center is full of information about how to deposit plasmids, store your plasmids or viral items, technical...

...in diverse bacteria Carol Gross’s lab recently deposited “Mobile-CRISPRi” plasmids, a set of modular CRISPRi...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

...posts on COVID-19 featuring plasmid tools from depositors who quickly shared their reagents: detection ...expression, and more. We also highlighted reagent repositories and other collaborative efforts in the scientific...detect SARS-CoV-2, including many with plasmids deposited at Addgene! 2. DNA Purification Without a Kit...

...522 organizations in 41 countries enriched our repository with 15,020 new plasmids — a testament to the...viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also...options. Almost any AAV transfer plasmid in our repository can now be packaged on demand in five distinct...

...Addgenies, we’re able to feature some recently deposited plasmids every quarter. The “hot plasmids” from...Academic Job Interview by Erik Snapp Addgene depositor and guest blogger Erik Snapp breaks down how to... blog posts Resources at Addgene Browse the repository Find educational resources at Addgene Search ...

... CHIME and new X-CHIME systems, developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide... scenarios. X-CHIME We recently developed and deposited four new systems to fill this gap. To enable context-specific...Figure from LaFleur, et al. 2024.   The X-CHIME deposit also includes pXPR_071, a plasmid that leads to...

...performing one.  At Addgene, over 120 labs have deposited CRISPR reagents, including many gRNA-containing...derived from scientists’ submissions, either their deposited gRNA plasmids or from submission of a spreadsheet...gRNA), application, cas9 species, PubMed ID, and depositor. The CRISPR applications we currently have data...

... successful, internationally renowned plasmid repository that it is today. Although I wish I could take... attributed to the entire Addgene family; the depositors and requesting scientists, all of the Addgene... It was great to see the faces of some of our depositing scientists who weren’t able to join us in person...

...and a domain needed for DNA unwinding. They’ve deposited plasmids for active and inactive CasX for E. coli...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

...bailii) and B3 (from Zygosaccharomyces bisporus.) Reporter constructs equivalent to site-STOP-site-myrRFP...cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested for excision efficiency...cross-reactivity; however, the other recombinase/reporter pairs were inactive, even at very high recombinase...

...Davidson's lab to coordinate the impressively big deposit. “He has every fluorescent protein under the sun...stage IV lung cancer that has now prompted him to deposit the tools he and his team created with the research...team who have made this large, important plasmid deposit possible.   ...

...background.” Just last month in Scientific Reports, Verkhusha’s team reported another advance in the application.... Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology...

...positive or more negative.  Neuronal stimulation: Depolarization When the inside of the neuron becomes positive...positive over a certain threshold, it becomes depolarized and will fire a signal. The non-specific cation...both cause ion movement that results in cell depolarization. They therefore function as a neuron “on switch...

...viral infection a) antibody-dependent complement deposition (ADCD) b) antibody-dependent cellular cytotoxicity...., 2019.    Antibody-dependent complement deposition (ADCD) Antibodies can bind to a pathogen and ...opsonization. In antibody-dependent complement deposition (ADCD), opsonization of IgG and IgM antibodies...

... the most frequently requested vectors in our repository and will discuss some of the features you may...Some of the more popular studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. ...animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g...

...bacteria, but have no fear - plasmids from Addgene depositors are making it easier than ever to use CRISPR  ...double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage of this...challenging problem than repression, scientists have deposited several plasmid tools that can be used in this...microbiome Beyond the human gut, the Barrick lab has deposited plasmids that can be used to engineer the bee ...

...plasmids and to receive important coronavirus-related deposits. Our valiant lab and shipping staff agreed to ...to come in and start getting plasmid deposits into the collection and to start shipping. They created the...also moved fast to ensure that the majority of deposited materials to study SARS-CoV-2 would be fully open...