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We narrowed to 475 results for: FLU

Showing: 181 - 200 of 475 results

  1. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...Choose the Brightest Fluorescent Protein Resources on Addgene.org Find Fluorescent Protein Plasmids Check...mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear yellow, and ..., which contains the tethering sites for the fluorescent proteins and is driven by an inducible promoter...

  2. Hot Plasmids - January 2023

    Type
    Blog Post
    ...speed of light over the last decade, but the fluorescent proteins (FPs) necessary for many of these applications...light and electron microscopy and observed high fluorescence resilience to these preparations. After crystalizing...    Fig. 4: hfYFP retains folding and fluorescence in high molarity GdnHCl, at high temperatures...!   Campbell, B.C., et al. Chemically stable fluorescent proteins for advanced microscopy. Nat Methods...

  3. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ...Genetically encoded voltage indicators (GEVIs) change fluorescence in real time when neurons or muscle cells transmit...However, technical difficulties such as small fluorescence changes or a requirement for very high excitation...sensors can detect action potentials in worms with fluorescence changes of up to 128% per 100 mV in the pharynx.... elegans membranes, which might beneficially influence the rhodopsin properties. Find these voltage biosensors...

  4. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...cytometry controls we’ll discuss are single color, fluorescence minus one (FMO), and isotype. You should always...should have a single color control for every fluorophore you intend to use in your panel. Using single...(C) with APC-Cy7.   FMOs FMO stands for fluorescence minus one. It is used as a negative control ...have the same Ig subclass, and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C...

  5. Deep Dive: qPCR

    Type
    Blog Post
    ...Agarose gel  LUX fluorogenic primers Low Single self-quenching fluorescent molecule connected to...normalize for that. Passive reference dyes are inert fluorescent dyes that are not dependent on amplification ... It is possible to multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to ... Tip🔥 The ABI machines automatically record fluorescent data on all channels - helpful if you forget ...

  6. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...found at the same site, is 2’-fluoro (2’-F), which substitutes a fluorine for the hydroxyl group at the...simple LED light. Lighting up your gRNA with fluorescent dyes In a scientific world where it often seems...seems like everything glows, why not spread the fluorescence to your gRNAs? Dye labeling the RNA in your ...

  7. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ... antigen-retrieval, and permeabilization will influence how the antibody interacts with its target and...conjugated to a detection substrate, such as a fluorophore, or a conjugated secondary antibody that will...of primary antibodies conjugated to different fluorophores or a panel of primaries that was raised in different...isotype or species, then you can choose different fluorophore-conjugated secondary antibodies specific to each...

  8. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators (GEVI...anion-conducting channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization...sensor and a mutated version of the pHLuorin fluorescent protein. This GEVI features increased brightness...

  9. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...agglomeration of fluorescent proteins into one particle within the cell, the lab fused the red fluorescent protein...different bee species. BTK can be used to introduce fluorescent markers to visualize localization and distribution...division counting (DCDC), relies on the assembly of fluorescent proteins into one bright particle that segregates...

  10. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-1-PV1917 105541...James M. Wilson AV-8-PV0105 105532-AAV8 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-8-PV0146 105535...Karl Deisseroth AV-5-PV0105 105532-AAV5 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-5-PV1090 105537-AAV5... M. Wilson AV-8-PV1302 105538-AAV8 pENN.AAV.TBG.PI.ffLuciferase.RBG James M. Wilson AV-8-PV3637 65015-...Karl Deisseroth AV-9-PV0105 105532-AAV9 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-9-PV0109 105533-AAV9...Karl Deisseroth AV-2-PV0105 105532-AAV2 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-1-PV3365 105554-AAV1...

  11. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...chemiluminescent or fluorescent detection, like horseradish peroxidase (HRP) or Alexa Fluor dyes, respectively...want to set up your blot with a polyvinylidene difluoride (PVDF) membrane, which is sturdier and retains...fresh chemiluminescent substrate or imaging your fluorescent probe. Then, if you don’t see any signal, you...

  12. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...Green or Taqman for signaling. Sybr Green is a fluorescent dye that works by binding to all double-stranded...while Taqman uses an intercalating dye, with a fluorescent probe and quencher to quantify specific amplicons...materials, and is not particularly sensitive. The fluorescent signal can be affected by AT-rich DNA and the... Sybr Green chemistry. Blue arrows represent fluorescent signaling in the presence of dsDNA. Image courtesy...

  13. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques. After...deliver ASAP5 to the cortex and imaged voltage fluctuations in awake behaving mice through two-photon microscopy...used to measure signal along the dendrite. D) Fluorescence signal as a function of the distance between...

  14. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ..., if your protein is fused with a fluorescent protein), fluorescence microscopy can theoretically be used... We screen the wells in groups as they become confluent, taking both early- and late-arising clones, and...Addgene.org Check Out Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors...

  16. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...generally not true. Because of the variations in fluorescent signals, and how the machine reads/interprets...light that “scatters” past the cell, instead of fluorescent markers. There are various types of SSC and FSC...which is comprised of antibodies conjugated to fluorescent markers and/or cellular dyes and stains. These...anti-CD14 and anti-HLA-DR antibodies conjugated to fluorescent markers are used to identify monocytes (upper...

  17. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator to... the sequence of interest and an inactivated fluorescent RNA reporter. If the target sequence is present...programmable RNA-binding protein, creating the fluorescent fusion protein dLwaCas13a-NF for in vivo RNA ...

  18. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...broad host range plasmids. The properties that influence host range are not fully understood, but scientists...also control copy number and stability, which influence the plasmid’s ability to establish and maintain...Enterobacteriaceae, Gluconacetobacter xylinus, Haemophilus influenzae, Hypomycrobium X, Legionella pneumophila, Methylophilus...xylinus, Paracoccous denitrificans, Pseudomonas fluorescens, P. putida, Rhizobium meliloti, R. leguminosarum...

  19. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...subcultured at densities that are low enough to prevent confluency but high enough to promote cell growth. Overgrown...detection kits that use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels...and discard it if the culture has become overly confluent or too old. Ensuring that you are working with...Martin, S., Merten, O.W., Galy, A., Fenard, D. ”Influence of mildly acidic pH conditions on the production...

  20. Five Popular Model Organisms

    Type
    Blog Post
    ...Transparency also allows scientists to easily observe fluorescently labelled proteins and tissues to better assess... into the worm, such as animals that express fluorescent markers (Mello et al., 1991). Genes can also ... genetic modification, such as expression of fluorescent protein or disruption of a gene, can be easily...Read our quick guide to Drosophila Download the fluorescent proteins 101 eBook Read our plasmids 101 blog...
Showing: 181 - 200 of 475 results