... Special Collections Fluorescent Protein Guide Fluorescent Protein Plasmids & Resources You...Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Fluorescent Protein Kits Blog: When ...with a variety of fluorescent proteins, as well as over 100 empty backbones. Fluorescent Protein Kits cpFRET...this guide to learn about the applications of fluorescent proteins and to find the plasmids that are available...Addgene's plasmid repository contains a variety of fluorescent protein plasmids. Use this guide to learn more...more about the many applications of fluorescent proteins (FPs) and to find the plasmids that are available...from Addgene's depositing scientists. Browse Fluorescent Protein Plasmids by Category Empty Backbones ...

... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal...levels and flux Nanosensor detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle. PLoS...Measure autophagic flux by pH-sensitive fluorescence (pMRX-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases... AAV Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties are ... Green fluorescence indicator of K+ for optical imaging (GINKO) Genetically encoded fluorescent indicators...AMP) Yellow fluorescent cAMP indicator (Flamindo2) Genetically-encoded yellow fluorescent cAMP indicator...cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based ...

...Plasmid Collections Fluorescent Proteins FRET Fluorescent Protein Guide: FRET More Fluorescent Protein Resources... is not required to be fluorescent. A variety of organic dyes and fluorescent proteins have been developed...Encoding Fluorescent Proteins for FRET The following plasmids can be used to create a fluorescent fusion...non-radiatively transferred from an excited donor fluorophore to an acceptor. Since the transfer of energy...efficiency of the energy transfer is measured using fluorescence microscopy by exciting the donor and measuring...to as intramolecular or unimolecular FRET). Fluorescent proteins can be used as donor and/or acceptor...of plasmids suitable for creating individual fluorescently tagged proteins to study protein-protein interactions...

... Plasmid Collections Fluorescent Proteins Activity Regulation Fluorescent Proteins: Activity Regulation...Plasmids using a fluorescent protein as a scaffold to allow scientists to selectively modulate gene expression...Regulation More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Biosensors Optogenetics... Optogenetics Background Fluorescent proteins such as GFP have long been used for labeling, but new methods...methods are emerging to use these fluorescent proteins to modulate biological activity. Early tools allow...GFP-lines for cell-specific manipulation. Plasmids Fluorescent Protein Purpose Principal Investigator Plasmids...

...Proteins Michael Davidson Fluorescent Protein Collection Michael Davidson Fluorescent Protein Collection More...Davidson lab fluorescent protein fusion constructs and backbones. Plasmid... Plasmid Collections Fluorescent Proteins...More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The lab ... collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs are of mammalian... corresponding empty backbone with different fluorescent tags for you to create fusion proteins with your...

...Imaging Guide Fluorescent Proteins: In Vivo Imaging More Fluorescent Protein Resources: Empty Backbones FRET...near-infrared, and photoactivatable fluorescent proteins. Plasmid... Plasmid Collections Fluorescent Proteins In Vivo Imaging...organs and whole mammals. Genetically encoded fluorescent probes are suitable for stable imaging of protein...undesirable background signals. Near-infrared fluorescent proteins for in vivo imaging are derived from...photoreceptors (BphPs). Spectrally distinct permanently fluorescent iRFP variants have been shown to have high effective...of the original iRFP has resulted in a split fluorescence complementation probe used for detecting protein-protein...

... Plasmid Collections Fluorescent Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization...Plasmids encoding fluorescent proteins tagged with genes or peptides with known subcellular localization...Localization More Fluorescent Protein Resources: Empty Backbones Allen Institute for Cell Science Plasmid...Plasmid Collection FRET Biosensors Optogenetics Fluorescent Protein Blog Posts Background General Cytoskeletal...localization. One of the most common is to use fluorescence microscopy to compare the localization of your...markers. Addgene has assembled a collection of fluorescent fusion proteins that are targeted to various ... should fuse your protein of interest with a fluorescent protein that has a different emission wavelength...

... Special Collections Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones...which fluorescence intensity is 50% of its maximum value. Maturation is the time for fluorescence to obtain...collection of empty plasmid backbones with different fluorescent tags for you to create fusion proteins with your...Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson...Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a ...a Monomer not a Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively...extensively by life scientists. The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et...

... curve with known amounts of protein. Fluorophores Fluorophores are light-sensitive molecules which emit...cell or tissue sample. Figure 6: Immunofluorescence, in which fluorophore-conjugated primary antibodies ...label cells in a sample. Immunofluorescence (IF) This method uses fluorophore-conjugated antibodies to...IF uses fluorophore color to differentiate between the targets of interest, if your fluorophore is bound...wells) by fluorescence activated cell sorting (FACS). Figure 7: Flow cytometry uses fluorescently-labeled...the fluorophores used is important. It is best to inversely pair the brightness of the fluorophore with...If a bright fluorophore is on an abundant target, it obscures fainter signals. Fluorophores can emit signals...

...genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru...An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru...Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal... AAV production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward...

...Photoconvertible , and Photoswitchable fluorescent proteins Various PIs Fluorescent protein fusion: contains empty...with N and C-terminal fluorescent protein tags that can be used to create fluorescent protein fusions with...cyan fluorescent protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent protein...yellow fluorescent protein variant (YPet) optimized for FRET measurements with a cyan fluorescent protein...pRSETa mEos2 20341 mEos2 (fluorescent protein) Loren Looger Photoactivable Fluorescent Protein Expression: ...blot. The plasmids in this collection contain fluorescent protein tags whose localization you can monitor...Technique/Tag PI Purpose Empty Backbones with Fluorescent protein tags Various Plasmids Blue/UV , Cyan ...

... tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Conformationally...BRET) CalFlux VNT : An intracellular calcium sensor created by fusing NanoLuc® and Venus fluorophore to ...Luciferase Plasmid Collection You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies... in Addgene’s collection are Firefly luciferase (Fluc), Renilla luciferase (Rluc), and NanoLuc® Luciferase...Nano-lanterns , Enhanced Nano-lanterns , and Antares : Fluorescent proteins are fused to Renilla luciferase (Nano-lanterns...-Leu linker to the circularly permuted Venus fluorophore. Learn more about pHlash sensor in our 28 Hot...luciferase with a V5 tag Kevin Janes 83281 pAAV-CAG-FLuc Firefly CAG AAV expression of firefly luciferase...

... Mehl 197576 pAcBac1-Mb-Fluoro-Phe B5 Pyl Fluoro-Phe B5 M. barkeri fluorinated phenylalanine derivatives... Mehl 197577 pAcBac1-Mb-Fluoro-Phe D6 Pyl Fluoro-Phe D6 M. barkeri fluorinated phenylalanine derivatives...5FTrp)RS Methanogenic archaeon 4-Fluoro-L-Tryptophan (4FTrp), 5-Fluoro-L-Tryptophan (5FTrp) Bacterial TAG...7FTrp)RS Methanogenic archaeon 6-Fluoro-L-Tryptophan (6FTrp), 7-Fluoro-L-Tryptophan (7FTrp) Bacterial TAG...TAG Chang Liu 85484 pDule-tfmF A65V S158A tri-fluoromethyl-phenylalanine synthetase M. jannaschii family...Wenshe Liu 137908 PylRS-AS PylRS M. mazei fluorophenylalanine derivatives Bacterial TAG Wenshe Liu 137976...163916 pXPR-SF61 SF5Phe tRNA synthetase para-Pentafluorosulfanyl-Phenylalanine Bacterial TAG Thomas Huber ...

...overexpress various fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources FP Tagging FP Overexpression...comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows...proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources External Resources...Goedhart J, Teusink B. In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep. 2019 Feb ... WA, Thorn KS. Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS...

...plasmids for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression...markers, somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells... Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish Whole organism lineage ...to study lipid dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch ...Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous genomic...consisting of plasmids with four promoters, six fluorophores, and empty vectors designed to streamline the...zebrafish for studying neurodegenerative disorders. Fluorescent Protein Fusion Construct Set - Amro Hamdoun Lab...

...Constitutive 5, 8 Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 Wilson 105536 ...Browse our control AAV collection, including fluorescent proteins, spaghetti monster, and more. Viral...Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These AAV can...EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None Other Cre-dependent color ...non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG...pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 CAG GFPsm_myc (does not fluoresce) Cre dependent 1 Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 CAG Ruby2sm-Flag (does not fluoresce) Cre dependent 1 Looger 45185 AAV-EF1a-BbTagBY...

... (Alias: CN4496) Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in... (Alias: CN2610) Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny... (Alias: CN2408) Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl... (Alias: CN2787) Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong... (Alias: CN3038) Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic...BGHpA (Alias: CN2700) Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium ...BGHpA (Alias: CN2237) Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium ...

...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology... Enterococcus sp. Geobacillus sp. Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium...Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles) West Nile virus ...systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression...expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning...

...Return to top Fluorescent Proteins The table below highlights plasmids that feature fluorescent or luminescent... You may also like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR...Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins Other Resources Background Addgene’s...from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids...Marillonnet and Johannes Stuttmann. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly...you are looking for, such as the promoter or fluorescent protein of choice. ID Plasmid Description Promoter...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable... sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to...: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging...dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal...imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for dynamic tracking...