We narrowed to 365 results for: Fus
-
TypeBlog Post...great way to do this is to use CRY2-CIB1 clustering. Fuse either CRY2 or CIB1 to your protein of interest ...light to generate CRY2-CIB1 oligomers that trap the fusion protein away from its usual location, preventing...
-
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...HiFi DNA Assembly (NEB) Cold Fusion Cloning (System Biosciences) In-fusion Cloning (Clontech) Although... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...readout. A common method of labeling proteins is to fuse them with a fluorescent reporter, such as GFP or... a transduction reporter. Note that eGFP is not fused to CD45, but is separately expressed. (The reason... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...must wait for the opsin to express and passively diffuse down the length of the fiber before stimulation...are not actively transported and must passively diffuse so, depending on the length of your projection,... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...published and documented by OMICtools. It must be confusing for a newcomer to decide on the right software...of ideas is what makes choosing the right tool confusing and it means that there is no one-size-fits-all... -
Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!
TypeBlog Post...libraries. Hot Plasmid 2: Transcription factor fusions modulate iPSC reprogramming efficiency. Listen ... -
Addgene’s New Way to Help You Share Science
TypeBlog Post...you have a question about culturing iPS cells? Confused by which system to use for genome editing? Know... -
CRISPR Cheat Sheet
TypeBlog Post...nucleases that cut or modify RNA as opposed to DNA. Fusing the nuclease to an adenosine deaminase can convert... -
The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing
TypeBlog Post...monoclonal antibody cell line, myeloma cells are fused with activated B cells from the spleen of mice injected... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog Post...substrate for a kinase of interest which is further fused to a bipartite nuclear localization signal (bNLS... -
Editor's Choice, August 2016
TypeBlog Post...catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at target... -
Scientist Networking: What is an Informational Interview?
TypeBlog Post...might say yes and they might say no but do not confuse networking for a job connection with ongoing relationship... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post... saturates around 30 uM. For low-abundance GFP fusions, a photoconversion signal may not be observed. ... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog Post...complete their work rather than overwhelmed or confused about what’s coming next. Goal setting and career... -
What's New in CRISPR - March 2020
TypeBlog Post...Casilio-ME1. Image from Taghbalout et al., 2019. Fusing epigenetic modifiers such as TET1 with inactive... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog Post...hard to find. This made the page look not only confusing, but also made it hard for users to get where ... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...orthologs NmCas9 and St1Cas9. Each ortholog was fused to a different fluorescent protein to create three... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post.... A Cre-estrogen receptor ligand binding domain-fusion can prevent this toxicity, but with the caveat ... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post...amino acids, but SpyCatcher is a protein. You can fuse SpyCatcher onto things, but even better if one could... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...chemogenetics relies on chemical activation and the diffusion of drugs throughout the body, the onset of receptor...