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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9 results for: Fus

Showing: 1 - 9 of 9 results

  1. CRISPR Guide

    Type
    Guide
    ...Molecular Cell , 73 (4), 714-726.e4. PMID: 30581144 Fu, Y., Foden, J. A., Khayter, C., Maeder, M. L., Reyon...Nature Biotechnology , 31 (9), 822–826. PMID: 23792628 Fu, Y., Sander, J. D., Reyon, D., Cascio, V. M., & Joung...Transcriptase (M-MLV RT) is fused to the C-terminus of Cas9 H840A nickase. The fusion enzyme can catalyze targeted...dCas9-VPR - dCas9 fused to several different activation domains in series; a single fusion protein, instead...constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects of CRISPRi/a may...Small Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9 Alternatives Resources Plan Your Experiment...This means proteins with other functions can be fused to a dCas9 and delivered to specific genomic locations...

  2. Optogenetics Guide

    Type
    Guide
    ...genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion to the nucleus upon light activiation... an optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed in neurons (red dots). ...point mutations, codon optimization, and chimeric fusion of domains from two different ChRs. These ChR variants... given protein, such as the Cre recombinase, is fused to Cry2 and the other half to CIB1, light-stimulated...-part transcription factors - Cry2 and CIB1 are fused to a transcriptional activation domain and DNA binding..., both LOV and Dronpa can control activity of a fused protein through allosteric interactions. Researchers...cryptochrome 2 (Cry2) from Arabidopsis thaliana is fused to truncated forms of cytosolic STIM1 to control...

  3. Modular Cloning Guide

    Type
    Guide
    ...possible combinations of pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs. FusX TALEN Assembly System...and ligated in a single step resulting in a 4-bp fusion site in between. MoClo can be used for a variety...coding sequences, terminators, etc.) flanked by fusion sites into individual Level 0 plasmids, or choose...Expression Paola Branduardi Easy-Modular Integrative fuSion-ready Expression toolkit for improved heterologous... a Cas9 nuclease, nickase, dCas9, or dCas9-FokI fusion for use in human or mouse systems. Fragmid Genome... Genome Engineering, TALEN Takashi Yamamoto Nine pFUS array vectors and destination vectors designed for...TALEN Stephen Ekker 336 plasmids of pre-assembled pFusX clones allowing for Golden Gate assembly of 15-16...

  4. Cloning

    Type
    Guide
    ...overhangs, or which are both blunt-ended, can then be fused together during a ligation reaction with T4 DNA ...different molecular biology techniques (such as fusing it with different tags, putting it under a variety...ready TOPO® vectors that has a DNA topoisomerase I fused to the 3’ end. The topoisomerase acts as a ligase...molecules using the 4 nucleotides and lastly DNA ligase fuses DNA strands together. In Gibson assembly DNA fragments.... Similar to Gibson, this method can efficiently fuse two (or more) fragments of dsDNA that have 30 or... of DNA that share no end homology can still be fused together in a seamless manner. To accomplish this... (or more) fragments of DNA that you would like fused along with custom ordered DNA oligos of 60-80 base...

  5. Plan Your Experiment

    Type
    Guide
    ...or Cas9 nickase fusions with different base editing proteins) Many base editors fused to Cas9 exert their...copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair...editing guide RNA (pegRNA) Prime editor (Cas9 nickase fused to reverse transcriptase (RT)) Can edit within a...permanently modifying the genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB...permanently modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64...transcription start site. CRISPRa requires dCas9 to be fused to transcriptional activators that need to be within...

  6. Lentiviral Vector Guide

    Type
    Guide
    ...the transfer plasmid contains a chimeric 5’ LTR fused to a heterologous promoter (often CMV or RSV), eliminating...Hybrid promoter; 5' LTR is partially deleted and fused to a heterologous enhancer/promoter such as CMV ...DNA integration and its effect on clinical pre-infusion CAR T-cell products. Journal of Translational ...

  7. Chemogenetics Guide

    Type
    Guide
    ...), or luminescent opsins, are opto-chemogenetic fusion proteins of a light-emitting luciferase and a light-sensing...modules, such as luciferase-fluorescent protein fusions, to more-sensitive opsins has led to improved LMOs...

  8. Antibody Guide

    Type
    Guide
    ...cultures using hybridomas, cell cultures created by fusing B cells with myeloma cells (immortalized B cells...fluorophores with minimal signaling overlap to avoid confusing data. Check that your machine has appropriate ...
Showing: 1 - 9 of 9 results