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We narrowed to 527 results for: HA tag

Showing: 181 - 200 of 527 results

  1. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...used for PL were APEX2 and BioID. APEX2 is fast, tagging proximal proteins within minutes, but requires ...being targeted via RNAi. Recently the Vellai lab has created an improved RNAi vector that leads to significantly...sequence of the toehold switch. For example, Green has also developed toehold switches to detect Zika virus...protein motifs than previously thought. Sidhu’s group has made several hundred SH3 domain containing plasmids.... In addition to these constructs, the Sidhu lab has made hundreds of additional plasmids available through...infect millions annually. Because it is highly contagious, it is essential to contain outbreaks when they...

  2. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...artifacts, it has better folding and maturation kinetics than other dark fluorescent proteins, it has good signal...encoding alternative promoters and/or fluorescent tags are available at Addgene. Shemesh OA, et. al. ...Technology (KAIST) and Institute for Basic Science (IBS) has developed another useful optogenetic tool that enables...facilitate future work with this goal, the Barrick lab has deposited the pDCAF3-IS5 plasmid containing a synthetic...operon originates from Pseudomonas putida CBB5 and has been tailored for expression in E. coli. This operon... contributed by Christina Mork The Murakoshi lab has developed two new FLIM-FRET acceptors, ShadowG, a... due to its very low quantum efficiency. ShadowY has a lower quantum yield and higher absorption than ...

  3. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ... expressing fluorescent proteins will be used to tag individual strains of bacteria. Their interactions... S. University of Baroda, India  Devarshi Gajjar has 10 years experience in the field of bacterial and...biomedicine and astrobiology. During the last 11 years he has studied various aspects extreme life on earth including...educational resources for scientists globally, Addgene has partnered with various community organizations and...

  4. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is our hope that by...Good bye 2020! What a year it has been for all of us. As we eagerly await 2021, we've looked back on ...

  5. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...The Rinehart Lab has developed several tools useful for the incorporation of phosphoserine into your ...Plasmid 68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297 GFP Q157TAG Plasmid 68296 GFP...68305 Beta lactamase S68TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68301 MBP-MEK1 S218TAG Plasmid 68300 MBP-MEK1... GFP S2TAG Plasmid 68295 GFP E17TAG Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ Plasmid 68291 SepOTSκ ...Plasmid 68283 SepOTSβ Plasmid 53225 MBP-MEK1 S218TAG/S222TAG ( aka. PCRT7 tetR pLtetO MBP-MEK1 XX Amp) Strain...Pooled Library 111704 Mode #1 Library Plasmid 69118 E17TAG GFP zeo resistance Plasmid 68307 SupD Strain 68306...BL21ΔserB Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 34623 SepOTSα SepRS/EF-Sep ( aka...

  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...elements – promoters, origins of replication, protein tags, and antibiotic resistance markers (just to name...restricted based on experimental constraints. This has been used for a wide range of purposes, including...Cre-containing adenovirus (Ad-Cre) or AAV (AAV-pgk-Cre) has been used to successfully introduce Cre into cells... can be an effective alternative to Cre-lox, and has also been used in conjunction with it, allowing for...recombination at the loxP recognition sites. A key advantage of the system is that Cre acts independently of...in certain cell or tissue types, during certain stages of development, or by making the Cre inducible ...

  7. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... purified them to near homogeneity using a 6XHis tag, and compared their specific activities by measuring... characterization of a fusion protein: Histidine tag, malate dehydrogenase, and green fluorescent protein... from R1 institutions to community colleges, and has helped provide original research experiences for ...

  8. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...CRISPR/Cas9 has made it easier than ever to make precise, targeted genome modifications. Cas9 has been modified...same genetic locus via co-expression of an epitope tagged dCas9 and antibody-activator fusion proteins. The...which one of Cas9’s two critical enzymatic residues has been converted to an alanine (D10A or H840A), changing... for your experiment, ensure your sgRNA sequence has little homology to other regions of the genome and...looser PAM requirements compared to SpCas9. xCas9 has a PAM requirement of NG, GAA, or GAT, making it a...SpG variant, discovered by the Kleinstiver group has a PAM of NGN (Walton et al., 2020). Further, this...reverse transcriptase, and a nCas9. While this system has been shown to be less efficient than base editing...

  9. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...your experiment. For example, does the fluorescent tag need to be pH stable or withstand a particular solute...enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration of small...cellular characteristics like pH, redox state, and voltage across membranes. These sensors generally follow...

  11. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...integrins, and more coming soon) as well as epitope tags like His, Flag, and Biotin. The antibodies developed...%}{% end_module_attribute %}{% module_attribute "tag" is_json="true" %}{% raw %}"module"{% endraw %}{%...complicated math. Bioengineering is a field that has been gaining a lot of traction in recent years. The... wealth of smaller engineered antibody fragments has further increased possibilities for synthetic antibody...coupled with iterative rounds of selection and mutagenesis to refine antibody performance. If you are interested...end_module_attribute %}{% module_attribute "wrap_field_tag" is_json="true" %}{% raw %}"div"{% endraw %...Wilson, D. S., & Keefe, A. D. (2000). Random mutagenesis by PCR. Current Protocols in Molecular Biology...

  12. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...enormously plastic tool and has taken the scientific world by storm. While Cas9 has been most widely used to..., Qi LS, Weissman JS, Vale RD (2014) A Protein-Tagging System for Signal Amplification in Gene Expression... to create specific edits in DNA, there has also been significant work on constructing Cas9 transcriptional...CRISPR activation for genes with known functions has the potential to be used in therapeutics, as it can...changes in phenotypes. For example, CRISPR activation has been used to differentiate induced pluripotent stem...creation of innovative technologies. To date his group has develop several popular CRISPR-based tools for altering...Genome-wide Screens with CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on Addgene.org Find...

  13. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...community has always emphasized the need for open science and collaboration. The field already has comprehensive...system a modified TIR-1 protein can degrade degron-tagged proteins in an auxin dependent manner. This system...first virtual organism in a computer.” This project has produced various resources for the worm community...

  14. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...screening, Single-cell Phenotypic Observation and Tagging with Light (SPOTlight) as a way to isolate individual...Cas12a. This is in part due to the fact that it only has a single active site. The RuvC active site in Casɸ...rely on multiple active sites. In addition, Casɸ has a minimal PAM requirement. For instance, for Casɸ...library here A new SaCas9 ABE variant (microABEI744) has improved on-target editing efficiency and reduced...

  15. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...dye, but has been modified with the addition of two iodine residues. Compared to MG, MG-2I has increased...development in real time.  In both cases, dL5** was tagged with mCerulean3 (mCer3) for visualization and expressed...xOtH5bs4HiI Advantages of MG-2I-dL5** over other photosensitizers One of the main advantages of MG-2I-dL5...

  16. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...and mammalian. These plasmids contain both untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids...that have been in the repository for many years, has now grown into a collection of over 2,400 plasmids... based on 100 structure-guided spike designs and has improved protein yields and stability. This stabilized...virus spreads around the world, the spike protein has had many opportunities to evolve. Several variants... to accelerate research through sharing reagents has never been so important.     References and ...

  17. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...controlled setting. Read more: Plasmids 101: Protein Tags Produce proteins that glow so that scientists ...be used in research or for therapeutics Addgene has compiled various educational resources to facilitate...plasmid) and typically include a gene that is advantageous for survival, such as an antibiotic resistance...

  18. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...looked hard at existing tools. As has been written here on Addgene, there has been a Cambrian-like explosion... for the CZ Biohub project by Manuel Leonetti to tag all 22,000 protein coding genes in the human genome...high-throughput design and analysis of CRISPR experiments. It has certainly boosted our productivity in the Biohub.... tools which were optimized for HDR, except for TagIn. HDR requires the additional step of mutating guide...cut-to-insert distance for HDR. (The notable exception is TagIn––see their "summarisation score".) For CrispyCrunch...

  19. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...insert FLAG tags into the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult...sequence between the microhomologies.MMEJ has certain advantages over HDR. Some species don’t have good ...vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new method for CRISPR ...of press. However, this process accounts for a percentage of the mutations seen with TALENs and CRISPR....follows. For increased versatility, it would be advantageous to adapt PITCh to insert genes into AAVS1, the...using the nuclease Cpf1. Since Cpf1 cuts in a staggered pattern, it is thought to be ideal for HDR-independent...and Ling Lu. (2016). “Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus...

  20. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...also the fastest test that has been developed so far.  A similar method has been optimized to detect the...Two-step-SHERLOCK has a sensitivity in the zeptomolar range while One-step-SHERLOCK has a sensitivity in...using lateral flow strips. Since the probes are tagged with streptavidin, the lateral flow strip first...State University. Since its appearance, SARS-CoV-2 has spread to almost every part of the world manifesting...full-fledged pandemic. Containing the spread of this virus has become an utmost priority for countries around the...the outbreak ultimately flattening the curve, it has become evident that widespread testing is crucial... 83 known COVID-positive samples tested, DETECTR has a positive prediction rate of 95% and a 100% negative...
Showing: 181 - 200 of 527 results