... CRISPRi toolkit for Burkholderia Silvia Cardona’s lab recently developed a CRISPRi toolkit for Burkholderia...this toolkit might be used in. Read the paper in ACS Synthetic Biology Find the CRISPRi toolkit SECURE...CRISPR-based toolkit for targeted genomic integration of fluorescent tags in neurons. Their toolkit named ORANGE...: CRISPRi in Burkholderia Base editors CRISPR toolkit for epitope tagging Lentiviral capsid-based Cas9...in bioRxiv Find Target-ACE   CRISPR/Cas9-based toolkit for epitope tagging in neurons Harold MacGillavry...neurons.  Read the paper in bioRxiv Find the ORANGE toolkit   Cas9/gRNA ribonucleoprotein delivery by lentiviral...

...Bee Microbiome Toolkit (BTK) to study bumble bee and honey bee microbiome. The toolkit consists of modular...Golden Gate Assembly. They built this toolkit upon the Yeast Toolkit by replacing the backbone vector’s ...Studying the bee microbiome using the Bee Microbiome Toolkit (BTK) Another system that lacks genetic tools is...used to study other microbiome systems. Find the toolkit for modular assembly of broad host range plasmids...engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids...

...that are sure to be enabled by this toolkit. For examples of the toolkit in action, see Cermak T, et al. ...Gateway-Compatible Toolbox is based on the original Tol2kit generated by the Chi-Bin Chien lab (Kwan et al....with TZAP Multiplexible plant genome engineering toolkit The Voytas Lab at the University of Minnesota recently...engineering across a variety of plant species. This toolkit includes TALEN and CRISPR nucleases, nickases, ...systems for multiplex gRNA expression. Expression of toolkit components can be controlled by a variety of promoters...cloning steps. The Voytas lab plans to update the toolkit frequently and has developed a website to aid in...

... al. RNA. 2018 PubMed PMID:29109157. Nanobody toolkit to monitor retrograde transport at the cell surface...contributed by Marcy Patrick  Listen to the nanobody toolkit podcast segment Nanobodies are single domain antibody...retrograde transport from the cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP...related fluorophores. Find all of the nanobody toolkit plasmids here. Buser DP, et. al. PNAS 2018. PubMed...

...plasmid deposit please sign up here.        New toolkit for N or C terminal tagging of proteins for degradation...2016). Now, the Kanemaki lab has created a new toolkit of plasmids for the N- or C- terminal tagging of... (Yesbolatova et al., 2019) using CRISPR. This toolkit makes it easy to tag your protein of interest to...Yesbolatova et al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods, 2019. PubMed...

...is being organized and funded by someone else (KittyBiome has been incorporated as a non-profit entity ...how to start applying their skills to it. The Kittybiome project gives them access to rich and complex...something interesting and contribute it back to the kittybiome project, but the primary goal is learning in ...www.openworm.org/ Cat Microbiome Project Website: http://www.kittybiome.com/   ...

...Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing in plants While there have been...nucleases. In doing so, they also created a modular toolkit for Cas-mediated genome engineering in plants. ...paper in bioRxiv Find the plant genome editing toolkit New Cas protein identified Jennifer Doudna’s ...

...CRISPR Corner New ready-to-use viral preps New toolkit for CRISPR-based genome editing in organoids By...members of the Rawlins Lab have compiled a new toolkit for performing genetics studies in organoids. The...together CRISPR-based approaches and organoid models, toolkits like this one provide new opportunities for investigating...

...applications. Despite the ubiquity of CRISPR/Cas toolkit, genome engineers continue to work diligently and...experimental successes and further develop the CRISPR/CAS toolkit by posting generic lab protocols, providing tips...

... discussion on the way to the office or in the kitchen, while sipping an afternoon coffee in between experiments...used and could bring back a taste of that casual kitchen chatter, ultimately sparking new ideas. Social ...

... Institute have expanded the genetic screening toolkit by optimizing a Cas12a-based method for screens...combinatorial genetic screens, and this optimized toolkit furthers Cas12a’s potential in future genetic screens...

..., and ColE2 plasmids) as cell division slows (Kittleson et al., 2011). Segall-Shapiro et al. showed that...biotechnology 36.4 (2018): 352. PubMed PMID: 29553576. 2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson...

... later, the Zhang lab expanded the RNA editing toolkit to allow C to U edits using their RESCUE (RNA Editing...addition of RESCUE further expands the RNA editing toolkit and sequences that can be edited.   As discussed...

...it in an application.     Figure 1: AAV toolkit for targeted gene delivery to the brain. The AAV...Miles T.F., Gradinaru V. Adeno-Associated Virus Toolkit to Target Diverse Brain Cells. Annual Review of...& Gradinaru, V. (2022). Adeno-Associated Virus Toolkit to Target Diverse Brain Cells. Annual Review of...

...protein-interacting RNA aptamers, such as in the CRISPRainbow kit . Using multiple copies of the aptamer improves stability...

...lymph nodes to activate T cells (Celli et al 2008, Kitano et al. 2016, Cavanagh et al. 2008)? Technical ...Immunity (2008): 119-126. PubMed PMID: 18370151. 16. Kitano, Masahiro, et al. "Imaging of the cross-presenting...

...approach; however, in practice integration can occur. Okita et al describes a protocol for generating iPSCs ...PubMed Central PMCID: 2677165. 3. Jia, Fangjun, Kitchener D. Wilson, Ning Sun, Deepak M. Gupta, Mei Huang...

... gut microbiomes titling it the Bee microbiome toolkit (Leonard et al., 2018).  Base Editing Among the...Engineering of Bee Gut Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids...

... protein for pseudotyping NanoMEDIC particles Akitsu Hotta pHCMV-RabiesG Contains Rabies virus ...

... the pictures below, yours truly is dressed as Unikitty from The Lego Movie). October was no less busy...