We narrowed to 222 results for: LRE
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TypeBlog Post... for iGluSnFR3 and JEDI-2P, several biosensors already in our collection have newly-available AAV preparations...
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Transferable Skills Guide: Time Management
TypeBlog Post...my time. As a busy researcher, you’ve probably already started coming up with time management techniques... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...Cell Reports 20:750–756 . https://doi.org/10.1016/j.celrep.2017.06.064 Carvalho LS, Turunen HT, Wassmer ... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post...many scFv-based therapies in clinical trials or already in the clinic (Bates and Power., 2019).Many of ... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog Post...starting by searching the literature to see what is already known about your housekeeping protein in your context... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post... If the distance is only 10 bp, the efficiency already drops by half and after only about 30 bp, it is... -
Site Directed Mutagenesis by PCR
TypeBlog Post...restriction site “A*” (in addition to the “A” site already in the parent vector). Also shown are the “B” restriction... -
Evolution of Lab Techniques
TypeBlog Post... even out into the field. Current versions are already commercially available although they are reported... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post... at the N-terminus, but you don’t have the tag already included in the vector you want to use. If you ... -
Hot Plasmids: Spring 2025
TypeBlog Post...test, and innovate faster than ever. Plus, we've already taken them for a test drive! We validated viral... -
Plasmids 101: Restriction Cloning
TypeBlog Post... SYBR Safe Pre and Post Blue or UV Light 0.5 GelRed Pre and Post (Post recommended) UV Light 0.1 ... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post... to optimize the serotype, except now you will already have a starting “optimal” injection volume and ... -
Antibodies 101: Validation
TypeBlog Post...large-scale proteomic and transcriptomic datasets already exist. Example: You want to use an antibody to... -
Important Considerations When Using AAVs
TypeBlog Post...in vivo. 3) The use of AAVs in humans AAVs are already being used to treat disease in humans and additional... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...Since CD45 is a common marker, finding an antibody already conjugated to my fluorophore of choice will not... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...adaptable system with lots of tools and reagents already in place. MoClo is a fantastic way to save time... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...Our marches across the world have had an impact already, gaining additional funding and the eyes of the... -
Typing CRISPR Systems
TypeBlog Post... candidates for CRISPR proteins. A type VII is already on the horizon (Altae-Tran et al., 2023)! References... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...which validated inhibitors or even approved drugs already exist. If you are lucky and find that a druggable... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...Addgene plasmids. His-tag labeling reagents You may already have a FRET acceptor-binding site on your protein...