We narrowed to 220 results for: LRE
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TypeBlog Post... for iGluSnFR3 and JEDI-2P, several biosensors already in our collection have newly-available AAV preparations...
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Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post...many scFv-based therapies in clinical trials or already in the clinic (Bates and Power., 2019).Many of ... -
Transferable Skills Guide: Time Management
TypeBlog Post...my time. As a busy researcher, you’ve probably already started coming up with time management techniques... -
Site Directed Mutagenesis by PCR
TypeBlog Post...restriction site “A*” (in addition to the “A” site already in the parent vector). Also shown are the “B” restriction... -
Evolution of Lab Techniques
TypeBlog Post... even out into the field. Current versions are already commercially available although they are reported... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post... at the N-terminus, but you don’t have the tag already included in the vector you want to use. If you ... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol...suitable with your protocols. FBS can be purchased already heat-inactivated, or it can be inactivated in the...dish. These cells will be added to the wells that already contain 0.5 mL of virus solutions at various dilution... -
Hot Plasmids: Spring 2025
TypeBlog Post...test, and innovate faster than ever. Plus, we've already taken them for a test drive! We validated viral... -
Plasmids 101: Restriction Cloning
TypeBlog Post... SYBR Safe Pre and Post Blue or UV Light 0.5 GelRed Pre and Post (Post recommended) UV Light 0.1 ... -
Important Considerations When Using AAVs
TypeBlog Post...in vivo. 3) The use of AAVs in humans AAVs are already being used to treat disease in humans and additional... -
Antibodies 101: Validation
TypeBlog Post...large-scale proteomic and transcriptomic datasets already exist. Example: You want to use an antibody to... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post... to optimize the serotype, except now you will already have a starting “optimal” injection volume and ... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...which validated inhibitors or even approved drugs already exist. If you are lucky and find that a druggable... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...Since CD45 is a common marker, finding an antibody already conjugated to my fluorophore of choice will not... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...adaptable system with lots of tools and reagents already in place. MoClo is a fantastic way to save time... -
Typing CRISPR Systems
TypeBlog Post... candidates for CRISPR proteins. A type VII is already on the horizon (Altae-Tran et al., 2023)! References... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...Our marches across the world have had an impact already, gaining additional funding and the eyes of the... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...Addgene plasmids. His-tag labeling reagents You may already have a FRET acceptor-binding site on your protein... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...opsin you use); the efficacy of optogenetics has already been proven. Therefore, a concrete, well defined... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog Post... guide RNA. But how to test DNA bases that are already snugly paired to each other? At this point, the...