We narrowed to 198 results for: LRE
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TypeBlog PostPublishedDec. 12, 2017, 3:13 p.m....nearly 400 times, and newcomer pCMV-ABE7.10 has already earned a blue flame for its adenine base editing...page in the Transcriptional Regulation category already has five blue flame plasmids covering three different...guide expression backbone with U6 promoter has already earned a blue flame. RNA Editing: Did anyone predict...
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Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m....available now? There are a wide variety of tools already available from Addgene as ready-to-use viral preps...of chemogenetic tools from Bryan Roth’s lab are already available allowing you to chemically control neuronal...of materials. At the time of publication we’ve already fulfilled more than 140 orders for virus! We’ll... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m.... adapt CRISPR for tagging experiments and it’s already looking like it’s making a big difference. We can...you seeing in this new tool? Mendenhall: We’ve already received many requests since we presented on this... speaking, anyone using CRISPR/Cas9 in the lab already should be able to run this protocol as well. We... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m....following our antibody blog posts, then you likely already have a good idea of the basics of immunoimaging...approach, primary antibodies must be either sourced already conjugated or conjugated in the lab. In the indirect...drawbacks of the indirect method, lengthening an often already lengthy process. (And while I have it on good authority... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m....unfairly, many believe that industry postdocs, having already been immersed in an industry environment, have ... of biotech. In other words, industry postdocs already have the prerequisite “industry experience” that...most jobs, it does help if you know someone who’s already in industry to land you that position. Work your... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m....running through each tool in detail, but that is already covered in the published articles. Instead, I have...generally recommend that laboratories that have already attempted several CRISPR experiments evaluate the...Bioconductor should be the first choice for anyone already familiar with R. For the Java enthusiasts, take... -
Your Top Requested Plasmid in 2015!
TypeBlog PostPublishedDec. 30, 2015, 3:30 p.m....repository nearly 1,000 times in 2015! If you haven’t already used it yourself, you’re probably wondering: What... half, a small sampling of the research it has already been used for includes work probing mTOR signaling... -
Seeing Red: Simple GFP Photoconversion
TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m....however, a GFP-tagged cell line or organism has already been made, and it would be expensive and time-consuming...discovery is likely to be most useful for labs that already have created a transgenic GFP-containing organism... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....and others in the audience discussed how J&J is already modifying cell and animal genomes to test hypotheses...panelists agreed that, for example, the technology is already available today to cure sickle cell anemia in stem... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....alpacas (Figure 1). At the time, researchers were already on a quest for smaller forms of antibodies and ...versatile family of tools, the applications they have already enabled, and the discoveries yet to come. ... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m....quality drug downstream. In some cases, there may already exist a drug which targets some of the candidate...CRISPR and drugs in the future While CRISPR has already done a lot for drug discovery, there is more on... -
10 Ways to Share Your Science!
TypeBlog PostPublishedMay 13, 2014, 8:34 p.m....10 ways to share your science 1) If you don’t already make it a habit to talk – really talk – about the... about and sharing some science on the one you already have. Read my earlier post about Scientists on ... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog PostPublishedAug. 4, 2020, 1:15 p.m....is now easily available through Addgene and has already been requested dozens of times in 2 weeks. This...Over 7,000 plasmids for COVID-19 research have already been requested from Addgene. Figure 2: Total... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m....existing therapeutics that are safe, effective, and already FDA-approved). This is a big job, but under the...FDA-approved or in clinical trials or preclinical studies already. We are following up on these leads as we speak... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....diverse biosensors? Well, a lot of these sensors are already engineered into viral vectors, so if you want to...virus with some of our most popular biosensors already packaged and ready to deliver. Ready to give biosensors... -
Viral Vectors 101: Viral Applications
TypeBlog PostPublishedApril 13, 2023, 1:15 p.m....customization is required, but many viral constructs have already been built and are available on the Addgene catalog...patients. Multiple viral-based gene therapies have already been approved by the FDA, and this mode of delivery... -
Transferable Skills Guide: Problem Solving
TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m....analysis. Conveniently, these are all skills you are already practicing in the lab. I like this definition...practicing systematic, logical thinking. If you are already a lab veteran at this point, you probably have ... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....and the Whitehead Institute, where salaries had already been raised higher previously and all 6,000+ postdocs...of postdocs’ lives. We know that many postdocs already made plans and budgets working around issues such... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m....expertise Fortunately, the eLife ambassadors group was already thinking about ways to tackle irreproducibility...planning next steps. April Clyburne-Sherin had already run several reproducibility workshops as Director... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog PostPublishedAug. 1, 2024, 1:15 p.m....better than a nitrocellulose membrane. If you have already run your blot using nitrocellulose, you may decide...avoid retaining residual antibodies. If you have already applied antibodies and need to dry the membrane...