We narrowed to 213 results for: Library

...CRISPR and Lentivirus Read about the ClonTracr Library Resources on Addgene.org Addgene's most popular...

...understand their functions. Read More Pooled Libraries Pooled libraries are single preparations of many different...given library have the same backbone, but they express or target different genes. Some libraries cover...Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced by the immune...

... lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...lentiviral CRISPR libraries. Figure 8: Overview of genome-wide screening using CRISPR libraries. What Are Pooled...Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous population ..., so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually optimized...activation and repression libraries target promoter or enhancer regions. As each library is unique, be sure ...sure to check the library information and original publication to see if a library is suitable for your experiment...CRISPR libraries have been developed for inhibition using dCas9. How Do You Use a CRISPR Library? CRISPR...

...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species Mammalian ...

...Guide Addgene’s Viral Service Lentiviral Pooled Libraries All Viral Plasmids Viral Vectors 101 eBook Biosafety...

...executed code in Jupyter notebooks using familiar libraries such as Pandas, Numpy, and Biopython.  We started...

...Recently, scientists used the genome-wide CRISPR libraries, Brunello (knock out) and Calabrese (activation...

...gRNA, is incubated with fragmented chromatin or library of purified DNA for purification of a specific ...

...Molecular Biology Reference Visit our protocol video library References Kim, Y., & Morrison, S.L. (2009). A...

...sorting (FACS) of E. coli transformed with aptamer libraries, the Jaffrey group selected for a 49-nucleotide...

...Cancer Collection Pages Learn How the ClonTracer Library Can be Used to Study Cancer Drug Resistance Plasmid...

...This post was contributed by Jim Woodgett. Public Library of Science (PLOS) created a stir earlier this year...

...sequencing can also be time-consuming, depending on the library prep and instrumentation used.  Here at Addgene...

...temperature-sensitive materials like viral preps and libraries were kept at the appropriate temperature for the...

...Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology, 115, ...

...Different Cas9 variants Learn about CRISPR Pooled Library Experiments Additional resources on the Addgene...

... into bacterial expression vectors to create a library. Bacteria were transformed, and then sorted by ...

... To find such a protein, they created a mutant library of E. coli tRNA adenosine deaminases (ecTadA), ...

...CRISPR Gene Editing Learn to Use CRISPR Pooled Libraries for Genome-Wide Screens Additional Resources...

...standard entry clone. ORFs from an ORFeome Gateway library or any PCR-amplified ORF cloned into pDONR221 can...carried out five rounds of random mutagenesis on a library of Arch mutants screening for improved brightness...Gagoski et al 2015). He has also constructed a library of eGFP-tagged human ORF clones to allow testing...