We narrowed to 222 results for: Library
-
TypeBlog Post...CRISPR and Lentivirus Read about the ClonTracr Library Resources on Addgene.org Addgene's most popular...
-
Science Guides
TypeGuide...understand their functions. Read More Pooled Libraries Pooled libraries are single preparations of many different...given library have the same backbone, but they express or target different genes. Some libraries cover...Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced by the immune... -
CRISPR Guide
TypeGuide... lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...lentiviral CRISPR libraries. Figure 8: Overview of genome-wide screening using CRISPR libraries. What Are Pooled...Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous population ..., so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually optimized...activation and repression libraries target promoter or enhancer regions. As each library is unique, be sure ...sure to check the library information and original publication to see if a library is suitable for your experiment...CRISPR libraries have been developed for inhibition using dCas9. How Do You Use a CRISPR Library? CRISPR... -
CRISPR Plasmids - Yeast
TypeCollection...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...sorting (FACS) of E. coli transformed with aptamer libraries, the Jaffrey group selected for a 49-nucleotide... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...gRNA, is incubated with fragmented chromatin or library of purified DNA for purification of a specific ... -
Bioinformatics at Addgene
TypeBlog Post...executed code in Jupyter notebooks using familiar libraries such as Pandas, Numpy, and Biopython. We started... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...Recently, scientists used the genome-wide CRISPR libraries, Brunello (knock out) and Calabrese (activation... -
DNA Purification Without a Kit
TypeBlog Post...Molecular Biology Reference Visit our protocol video library References Kim, Y., & Morrison, S.L. (2009). A... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...Cancer Collection Pages Learn How the ClonTracer Library Can be Used to Study Cancer Drug Resistance Plasmid... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...This post was contributed by Jim Woodgett. Public Library of Science (PLOS) created a stir earlier this year... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post... into bacterial expression vectors to create a library. Bacteria were transformed, and then sorted by ... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...Different Cas9 variants Learn about CRISPR Pooled Library Experiments Additional resources on the Addgene... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...temperature-sensitive materials like viral preps and libraries were kept at the appropriate temperature for the... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology, 115, ... -
QC Sequencing Technologies at Addgene
TypeBlog Post...sequencing can also be time-consuming, depending on the library prep and instrumentation used. Here at Addgene... -
Bacterial Expression Systems
TypeCollection...MoClo Toolkit (Addgene #1000000080) features a library of promoters and purification tags compatible with...Biosensors Kit (Addgene #1000000021) is a vector library for the generation of FRET biosensors for ratiometric... -
Tetracycline Inducible Expression
TypeCollection...Takeshi Imai 155257 Watermelon Pooled Library Lentiviral barcode library for simultaneous tracing of clonal... -
CRISPR History and Development for Genome Engineering
TypeCollection...especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are important...important to a given phenotype. Current libraries are available for gene knockout, as well as transcriptional... -
CRISPR Plasmids - Bacteria
TypeCollection...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...