We narrowed to 7 results for: Library
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TypeProtocol... like... Pooled libraries Guide to Using Pooled Libraries All CRISPR Pooled Libraries Molecular Biology... Protocols CRISPR Library Amplification CRISPR Library Amplification...process my Addgene pooled library? Introduction Please refer to our pooled library material pages for amplification... for CRISPR libraries. This protocol allows the amplification of a pooled-plasmid library in Escherichia...obtained the pooled library from Addgene, these protocols can be found on the Pooled Library page for the specific...pooled library amplification protocol is expected to obtain satisfactory results for libraries up to 200,000... uptake of plasmid library DNA. This quantity of cells is sufficient for libraries up to 200,000 individual...
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Video Library
TypeProtocol -
Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps
TypeProtocol...Watch the Video! CRISPR Library Amplification Amplify CRISPR pooled-plasmid libraries Diagnostic Restriction... -
Plasmid Cloning by PCR (with Protocols)
TypeProtocol...plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient to give...you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate your PCR product from the... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol...described in Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang... -
Isolating a Monoclonal Cell Population by Limiting Dilution
TypeProtocol...described in Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...backbone upon which The RNAi Consortium has built a library of shRNAs directed against 15,000 human and 15,... 2003. 115:209-216. PubMed . A lentiviral RNAi library for human and mouse genes applied to an arrayed...