We narrowed to 379 results for: Lor;
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TypeBlog Post...’ll choose to study Crepidula atrasolea or Prochlorococcus with your next project. Gymnotiformes and Mormyridae...evolution in immunology, neurobiology, and more. Prochlorococcus (Penny Chisholm) This cyanobacterium is the...
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Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog Post...experiment and choice of loading control. First, let’s explore why these assumptions are key to using loading ...loading control. Thanks for joining us in this exploration of western blot normalization. May your blots... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...into the content of Addgene’s aptamer soup by exploring the development and applications of fluorescent...aptamers Just as vegetables come in a variety of colors, so do aptamers! Corn was recently described in... -
10 Steps to a Perfect Science Talk
TypeBlog Post...Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion Don’t just plan to reuse...about the particular audience and venue before tailoring each presentation appropriately. Find out who... -
Site Directed Mutagenesis by PCR
TypeBlog Post...specific nucleotide substitutions (or deletions) in a tailored manner. The approach can be used in conventional...plasmids, but may also facilitate genome editing. Tailored mutations are commonly introduced to endogeneous... -
Evolution of Lab Techniques
TypeBlog Post...fluorescence for fluorescently labeled probes. Colorimetric and luminescence based detection methods are...and behavior. References 1. Southern, Edwin Mellor. "Detection of specific sequences among DNA fragments... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post...of carbohydrate binding proteins waiting to be explored and exploited. With better characterization, these...Jessica C. Sacher, and Christine M. Szymanski. "Exploring the interactions between bacteriophage-encoded... -
A History of Genome Engineering in Popular Culture
TypeBlog Post... work The Island of Dr. Moreau (1896) where he explores the concepts of animal testing and modifications...but the common themes that these authors were exploring were space and robots. Warping to stars in space... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...chemical reactions and this induces a noticeable color change. This method has high sensitivity but low...Molecular Biology (University of Milan, Italy) and a Bachelor's degree in Biotechnology. References Degeling... -
Typing CRISPR Systems
TypeBlog Post...effector — Cas9, Cas12, or Cas13. If you’d like to explore Class 2 CRISPR in more depth, the Jennifer Doudna...8(1). https://doi.org/10.1186/s13578-018-0255-x Taylor, H. N., Laderman, E., Armbrust, M., Hallmark, T... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...Rhodamine 123 (R123) (Liu et al., 2017). In multi-color imaging, it is essential to select dyes with different...applications. Other current advancements include work exploring far-red-emitting dyes in bacterial imaging, offering... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post...fluorophores can add a second layer of contrast, color contrast, to an image and thereby provide molecular...Microscopy Choosing Your Fluorescent Proteins for Multi-Color Imaging Choose the Brightest Fluorescent Protein... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post...antibodies (i.e., immunofluorescence), or by a colorimetric reaction facilitated by an enzymatic reaction...Cruse JM, Lewis RE, Wang H, Geziena MT, Steven GE, Lorna J. (2004) ANTIGENS, IMMUNOGENS, VACCINES, AND IMMUNIZATION... -
Protocol - How to Perform Sequence Analysis
TypeProtocol...graphically depicts the sequence as a series of colored peaks corresponding to one of the four nucleotide... -
Protocol - How to Inoculate a Bacterial Culture
TypeProtocol...mL Bleocin 5 µg/mL Carbenicillin 100 µg/mL Chloramphenicol 25 µg/mL Coumermycin 25 µg/mL Gentamycin 10... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post... of two plasmids: pCas9: carries Cas9 and chloramphenicol resistance pCRISPR: carries a spacer targeting...variety of bioactive natural products. To easily explore and engineer pathways within this genus, Huimin... -
Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps
TypeProtocol... the Video! DNA Purification Miniprep, phenol-chloroform extract, and precipitate DNA DNA Quantification... -
Using AAV for Neuronal Tracing
TypeBlog Post...Traditional tracing compounds either produce a colorimetric product or are fluorescent. While dyes usually...ratios, making these neurons fluoresce in different colors. An individual neuron can then be traced throughout... -
Immunology Research Plasmids and Resources
TypeCollection...inflammation, the different cytokines that are released tailor the immune response to a specific trigger. Interferons...inflammation, the different cytokines that are released tailor the immune response to a specific trigger. Symbol... -
Using a Light Microscope Protocol
TypeProtocol... the sample or b) using dyes or stains that add color to some components of the sample but not others....