We narrowed to 417 results for: NUC

.... Dopamine-glutamate neuron projections to the nucleus accumbens medial shell and behavioral switching...Cortical-Thalamic-Striatal Circuits through the Parafascicular Nucleus. Neuron 102(3):636-652. PubMed (Link opens in a...

...that are comprised of cells with a large nucleus, large nucleoli, and little cytoplasm. Positive staining...completely removed.  Replicating oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids...

... screen the activity of arrays of zinc finger nucleases. Addgene Blog Introduction to FRET Bimolecular...activity Fluorescence (mCherry) Fusobacterium nucleatum Jörg Vogel 46002 pGR Terminator strength Fluorescence...

...plasmids The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun...Baldwin Fibroblasts iTSCs Lentiviral Mouse Extensive Nuclear Reprogramming Underlies Lineage Conversion into...

...tandem. They went on to show that their CRISPR/Cas9-nuclease and CRISPR/Cas9-nickase vectors work successfully...Currently, the kit only includes two versions of Cas9, nuclease and nickase, but we are now adopting dCas9, dCas9...

...important that the preparation is pure from other nucleic acids (genomic DNA and RNA), proteins and chemical...is the purity. In addition to being free of any nucleic acid and chemical contaminants, these DNA samples...

...plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though ...restriction sites into which you can clone your 20 nucleotide guide sequence of choice (for generation of a...

...variants share the presence of a predicted RuvC nuclease domain, characteristic of CRISPR-Cas enzymes. ...than Cas13 or Cas12 are for the other types of nucleic acids and required a certain sequence specificity...

...2011, from zinc finger nucleases to transcription activator-like effector nucleases (TALENs). Our most popular...

...Soup by Aliyah Weinstein RNA aptamers, which are nucleic acid sequences that fluoresce upon binding a small...Transfection by Alyssa Cecchetelli The introduction of nucleic acids into bacteria or eukaryotic cells is a common...

...addition of 4-hydroxytamoxifen, it can move to the nucleus for activity. They also developed one of these ...gene activation and editing. Read the paper in Nucleic Acids Research Find the drug inducible Cas9 plasmids...

...repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders the NLS...NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress gene expression. In the...

...and binds the inverted repeats (green); and an endonuclease reaction occurs which cuts the DNA.3.) The released...transposon can now bind a strand of DNA with a TA dinucleotide (there are many such sites in the human genome...

...engineering from ZFNs through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the...described a chimeric Cas9 that is improving the nuclease specificity dramatically and reducing off-target...

... IgnaviCas9 is a hyperthermostable Cas9 CRISPR nuclease that is active up to 100°C.  The IgnaviCas9 protein...for long time periods and workflows where CRISPR nuclease activity is desired and time or protocol steps...

...tertiary structure when transcribed which aids in nuclear export of mRNA and helps increase the expression...The polyadenylation or polyA signal aids in the nuclear export of RNA and RNA translation, and promotes...

...adeno-associated virus particles containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire...Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32...

...green cloud) functions both as a restriction endonuclease and as a ligase by cleaving and rejoining supercoiled... 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase....

...catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled with a customizable single ...RNA (scRNA) will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit a transcriptional...

...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...