Skip to main content

We narrowed to 417 results for: NUC

Showing: 181 - 200 of 417 results
  1. Deisseroth INTRSECT Collection

    Type
    Collection
    .... Dopamine-glutamate neuron projections to the nucleus accumbens medial shell and behavioral switching...Cortical-Thalamic-Striatal Circuits through the Parafascicular Nucleus. Neuron 102(3):636-652. PubMed (Link opens in a...
  2. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...that are comprised of cells with a large nucleus, large nucleoli, and little cytoplasm. Positive staining...completely removed.  Replicating oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids...
  3. Bacterial Expression Systems

    Type
    Collection
    ... screen the activity of arrays of zinc finger nucleases. Addgene Blog Introduction to FRET Bimolecular...activity Fluorescence (mCherry) Fusobacterium nucleatum Jörg Vogel 46002 pGR Terminator strength Fluorescence...
  4. Plasmids for Stem Cell Research

    Type
    Collection
    ...plasmids The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun...Baldwin Fibroblasts iTSCs Lentiviral Mouse Extensive Nuclear Reprogramming Underlies Lineage Conversion into...
  5. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though ...restriction sites into which you can clone your 20 nucleotide guide sequence of choice (for generation of a...
  6. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...Soup by Aliyah Weinstein RNA aptamers, which are nucleic acid sequences that fluoresce upon binding a small...Transfection by Alyssa Cecchetelli The introduction of nucleic acids into bacteria or eukaryotic cells is a common...
  7. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...addition of 4-hydroxytamoxifen, it can move to the nucleus for activity. They also developed one of these ...gene activation and editing. Read the paper in Nucleic Acids Research Find the drug inducible Cas9 plasmids...
  8. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders the NLS...NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress gene expression. In the...
  9. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...and binds the inverted repeats (green); and an endonuclease reaction occurs which cuts the DNA.3.) The released...transposon can now bind a strand of DNA with a TA dinucleotide (there are many such sites in the human genome...
  10. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...tertiary structure when transcribed which aids in nuclear export of mRNA and helps increase the expression...The polyadenylation or polyA signal aids in the nuclear export of RNA and RNA translation, and promotes...
  11. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...green cloud) functions both as a restriction endonuclease and as a ligase by cleaving and rejoining supercoiled... 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase....
  12. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...
Showing: 181 - 200 of 417 results