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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 208 results for: PHI-1

Showing: 181 - 200 of 208 results

  2. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ... cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis...Halstead et al. used TRICK to examine translation of Drosophila patterning gene oskar. Early in development, ...

  3. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...defunct (but fun) plug-in called Maps.  Figure 1: See a starfish? This is actually a map of Joanne ...Networking connections can last years This is a graphic snapshot of my LinkedIn network from a few years...

  4. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...vector-exosomes), exosome AAVs, or exo-AAVs (Figure 1). The researchers discovered that these particles ...coagulation factor IX in liver in a mouse model of Haemophilia B (Meliani et al., 2017). Exo-AAV9 was successfully...

  5. Components of CRISPR/Cas9

    Type
    Blog Post
    ...individual protein has all the components necessary to: 1. Bind to a guide RNA The guide RNA, which will be ...meningitidis (NM) 3' NNNNGATT  Streptococcus thermophilus (ST) 3' NNAGAAW  Treponema denticola (TD...

  6. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...and also by a lactose analogue, IPTG (isopropyl β-D-1-thiogalactopyranoside). (To be completely accurate...reproduce to form colonies (used to complement auxotrophic cell lines), or genes which rescue a lethal phenotype...

  7. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ... UK team members before they log off for the day. 1:45 PM--Write final report. Work on a final report ...what the day to day looks like. Check out the infographic for an outline of my day or find the text version...

  8. Antibodies 101: Isotypes

    Type
    Blog Post
    ...region: IgM, IgD, IgG, IgE, and IgA.    Figure 1: Diagram of an antibody labeled with Fc, Fab, heavy...found bound to IgE receptors on the surface of basophils and mast cells, two types of white blood cells...

  9. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...and CasPEDIA have got you covered!     Fig. 1 – CasPEDIA Cas ID display information for a given ...to relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas...

  14. CRISPR in the Clinic

    Type
    Blog Post
    ...significant progress in clinical trials.     Figure 1: Types of delivery and editing strategies for CRISPR...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...

  15. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...viruses - 2 cas systems regulate at the DNA level and 1 at the RNA level. Udi Qimron from Tel Aviv University...invasion.  His lab is studying P. furiosus, a hyperthermophile that exists in sea vents at the boiling point...

  16. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... – a mechanical device that could be used to bend 1/8th inch steel wire into a backbone structure of a...popularity was short-lived as powerful molecular graphics packages were soon developed that allowed researchers...

  17. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...target DNA quickly and accurately.  Figure 1: Steps in PCR reaction   This process of denaturation...relies on heat resistant DNA polymerase from the thermophilic bacterium, Thermos aquaticus (Taq). Taq polymerase...

  19. Promoters

    Type
    Guide
    ...Strong mammalian promoter from human elongation factor 1 alpha CAG Constitutive Strong hybrid mammalian promoter...promoter for small RNA expression UAS Specific Drosophila promoter containing Gal4 binding sites Bacterial...

  20. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse genome...shown that a Cas9 protein from Geobacillus stearothermophilus is more stable in human plasma than the standard...
Showing: 181 - 200 of 208 results