We narrowed to 207 results for: PHI-1;

...region: IgM, IgD, IgG, IgE, and IgA.    Figure 1: Diagram of an antibody labeled with Fc, Fab, heavy...found bound to IgE receptors on the surface of basophils and mast cells, two types of white blood cells...

...and CasPEDIA have got you covered!     Fig. 1 – CasPEDIA Cas ID display information for a given ...to relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas...

...prove the technology. This transition takes around 1 – 2 years. The product is the measure of success...Business - Posted on Nature.com, contributed by Julia Phillips and Fiona Beckman Acknowledgments Thank you ...

... trafficking of these biosensors.   Figure 1: Schematic of iGluSnFR3 (left). GluSnFR3 in pMinDisplay... calcium signaling related fluorescence during morphine administration and withdrawal to explore how these...

...see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system...repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time ...

...first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple catalogue...follow him on Twitter @CMacPhD.          References  1. Ghorbal, Mehdi, et al. "Genome editing in the human... not. It is an offline tool, but comes with a graphical user interface. Best strategy for labs: Define...

...significant progress in clinical trials.     Figure 1: Types of delivery and editing strategies for CRISPR...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...

...viruses - 2 cas systems regulate at the DNA level and 1 at the RNA level. Udi Qimron from Tel Aviv University...invasion.  His lab is studying P. furiosus, a hyperthermophile that exists in sea vents at the boiling point...

... – a mechanical device that could be used to bend 1/8th inch steel wire into a backbone structure of a...popularity was short-lived as powerful molecular graphics packages were soon developed that allowed researchers...

...editing machinery such as CRISPR-Cas. Figure 1: Schematic of targeted gene knock-out (left) and targeted... WM, Curiel DT (2019) Long-term correction of hemophilia B using adenoviral delivery of CRISPR/Cas9. Journal...

...target DNA quickly and accurately.  Figure 1: Steps in PCR reaction   This process of denaturation...relies on heat resistant DNA polymerase from the thermophilic bacterium, Thermos aquaticus (Taq). Taq polymerase...

...genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse genome...shown that a Cas9 protein from Geobacillus stearothermophilus is more stable in human plasma than the standard...

... digging deeply into their data.     Fig. 1: Genomic fraction for each of five major ancestry ...have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic...

... reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other components. Image from... from their assay to Addgene.  Drew Endy’s and Philippa Marrack’s labs also deposited plasmids for producing...

...fun together! Laura’s Top 10 Event Planning Tips! 1. Take suggestions Some of Addgene’s best events have...culture At Addgene we organically developed the philosophy of  “work hard, play hard.” Part of the reason...

...testing. You can find Nick on LinkedIn. References 1. Skloot, Rebecca, and Bahni Turpin. The immortal life...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...

... done and we’re onto the next story. Figure 1: Addgene’s Agile Approach Sharing is caring There... Sophia K. Cheng...

...SpCas9 base editors with editing windows as small as 1-2 nucleotides! To reduce off-target effects associated...Lower on-target activity than BE3; editing window ~1-2 nt  87438  pET42b-HF-BE3  HF-Cas9 (NGG)  Very ...compatible with two-photon microscopy using Ti-Sapphire lasers. pBAD-RDSmCherry1 is a red-shifted variant...

...indel formation and phenotypic changes as early as 1 week post-injection. Livers from these mice were histologically... use of St1Cas9 (~3.3 kb) from Streptococcus thermophilus and a rationally-designed truncated Cas9. Unfortunately...

...halorhodopsins to silence neuronal activity (Figure 1) (Wiegert et al., 2017). How to get an opsin into...2011): 26. PubMed PMID: 21191368. Deubner, Jan, Philippe Coulon, and Ilka Diester. "Optogenetic approaches...