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  1. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...for your idea. Our product team has implemented a policy of never rejecting an idea simply because it's ...innovative group. Our entire company benefits from their policy! Perfectionism When we reach for perfection, our...

  2. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...ensure this tRNA processing only occurs on the RNA polymerase III transcript, the sgRNAs were encoded on the...donor site; SAS, splice acceptor site; pA, polyadenylation signal. Image reused from Riedmayr et al. 2023...

  3. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...preparation for updating this post, I took an informal poll of Addgene hiring managers. All of them stated that...through in their resumes alone.  In my unofficial poll, hiring managers also said that they considered ...

  4. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...producing lentivirus in Lenti-X 293T cells using a polyethyenimine transfection protocol. I also recommend searching...incubation time, antibiotic conditions, and amount of polybrene. (psst…didn’t get your library pre-made from Addgene...

  6. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...exposed to specific UV wavelengths or chemicals (Polstein and Gersbach, 2015; Zetsche et al., 2015). These...e7 . https://doi.org/10.1016/j.cels.2018.04.011 Polstein LR, Gersbach CA (2015) A light-inducible CRISPR-Cas9...

  8. CRISPR References and Information

    Type
    Collection
    ...donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF 583.3 KB Zhang gRNA cloning CRISPR RNA...

  9. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Anuganti, M., Zhang, R., Lloyd, K., Jardine, J., Coppola, J., Meijers, R., Li, J., & Springer, T. A. (2024...2024.01.26.577394 Jo, M. H., Li, J., Jaumouillé, V., Hao, Y., Coppola, J., Yan, J., Waterman, C. M., Springer, T. A.,...

  11. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...guide to working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org ...

  13. Caltech Systemic Capsids

    Type
    Collection
    ...Dimidschstein 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Control Feng 163505 CN1390-rAAV-DLX2.0...

  15. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...thaliana dCas9 Activation of gene transcription Polstein et al., 2015 LACE CRY2 and CIB1 from A. thaliana...https://doi.org/10.1016/j.chembiol.2014.12.011 Polstein LR, Gersbach CA (2015) A light-inducible CRISPR-Cas9...

  16. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Bradley 3rd ~9 pairs/exon 9,508 Mouse CRISPR Proximal Poly(A) Site Knockout Library 190744 Knockout Mouse Bradley...

  18. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...chain constant domain (C L ) and the κ light chain polyA tail sequences (κ pA), a CMV promoter for heavy ... depending on the plasmid, followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody ...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...of assembling these multi-protein fusions or “polyproteams”, the Howarth lab created the MBPx-SpyCatcher...sequential additions have been made, the final polyproteam can be eluted using maltose. This procedure allows...
Showing: 181 - 200 of 362 results