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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 365 results for: Pol;

Showing: 181 - 200 of 365 results

  1. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...where they are incorporated into the growing polypeptide chain. One part of the tRNA contains the anticodon... codon for every amino acid across an entire polypeptide. Many researchers have created codon-optimization...

  2. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...sample is first amplified using RPA (recombinase polymerase amplification) or reverse transcriptase (RT)-...as well as health-related single nucleotide polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides...

  3. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...for your idea. Our product team has implemented a policy of never rejecting an idea simply because it's ...innovative group. Our entire company benefits from their policy! Perfectionism When we reach for perfection, our...

  4. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...producing lentivirus in Lenti-X 293T cells using a polyethyenimine transfection protocol. I also recommend searching...incubation time, antibiotic conditions, and amount of polybrene. (psst…didn’t get your library pre-made from Addgene...

  5. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...applications, including detection of environmental pollutants, drug discovery, and disease diagnosis. As biosensors...electrochemical sensors for emerging environmental pollutants.” Front Chem. 2014; 2: 41. PubMed PMID: 25019067...

  6. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...ensure this tRNA processing only occurs on the RNA polymerase III transcript, the sgRNAs were encoded on the...donor site; SAS, splice acceptor site; pA, polyadenylation signal. Image reused from Riedmayr et al. 2023...

  7. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...preparation for updating this post, I took an informal poll of Addgene hiring managers. All of them stated that...through in their resumes alone.  In my unofficial poll, hiring managers also said that they considered ...

  8. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...exposed to specific UV wavelengths or chemicals (Polstein and Gersbach, 2015; Zetsche et al., 2015). These...e7 . https://doi.org/10.1016/j.cels.2018.04.011 Polstein LR, Gersbach CA (2015) A light-inducible CRISPR-Cas9...

  10. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Anuganti, M., Zhang, R., Lloyd, K., Jardine, J., Coppola, J., Meijers, R., Li, J., & Springer, T. A. (2024...2024.01.26.577394 Jo, M. H., Li, J., Jaumouillé, V., Hao, Y., Coppola, J., Yan, J., Waterman, C. M., Springer, T. A.,...

  11. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...Modular by design: Key elements (promoter, tag, polyA site) are flanked by unique restriction sites so...general heat-shock proteins using IP with CycT1 or PolII-CTD antibodies and unlabeled MS in DLD-1 cells (...

  13. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...guide to working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org ...

  15. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...chain constant domain (C L ) and the κ light chain polyA tail sequences (κ pA), a CMV promoter for heavy ... depending on the plasmid, followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody ...

  17. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...thaliana dCas9 Activation of gene transcription Polstein et al., 2015 LACE CRY2 and CIB1 from A. thaliana...https://doi.org/10.1016/j.chembiol.2014.12.011 Polstein LR, Gersbach CA (2015) A light-inducible CRISPR-Cas9...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...of assembling these multi-protein fusions or “polyproteams”, the Howarth lab created the MBPx-SpyCatcher...sequential additions have been made, the final polyproteam can be eluted using maltose. This procedure allows...
Showing: 181 - 200 of 365 results