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We narrowed to 578 results for: SUB

Showing: 181 - 200 of 578 results

  1. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ... tested the ability of GeoCas9 RNPs to cleave substrate when the reaction was carried out at elevated ... in diluted human plasma for 8 hours at 37°C. Substrate was then added and enzyme activity measured. SpCas9...reaction buffer for 8 hours at either 37 °C or 4 °C. Substrate was then added and cleavage product analyzed on...

  2. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...targeted to specific tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches excel... excel at discrete cell or tissue-subregion targeting down to the limits of light delivery. Chemogenetic...DR If precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad...

  3. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...total of eight evolutionarily conserved CRISPR subtypes comprised of more than 40 cas gene families have...grouped the systems into three distinct types and subtypes using polythetic classification based on evolutionary...system and are not translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR ...

  4. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...labs. This week we are pleased to announce the submission of the results of our first study which can be...PubMed PMID: 16528756. 2. Oh, Steve KW, et al. "Substantial overproduction of antibodies by applying osmotic...Amin Hajitou. "Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated...

  5. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers to Scientific...Common ones include age range, familiarity with the subject, shared interests, socioeconomic and/or racial/...

  6. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ... (Fig. 1). However, once thawed, there was a substantial reduction (from 7.4 to 5.0) in the pH of samples...periods, and measured the pH. We found that tubes subjected to a recovery period of at least 96 hours had ...infectivity of the virus. However, there was a substantial reduction in infectivity after 3 weeks of exposure...

  7. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...temperature ranges and make them grow better at suboptimal ones, which could allow even your regular E. ...worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse, our ...mesophilic chaperones would not function well under suboptimal conditions. This means recombinant proteins can...

  8. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...recombineering to create insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly ...amplicons based on sequence homology, and the subsequent product is then digested and ligated into an ...nick repair is needed. Since the bacteria are subsequently grown at 37 °C, the recombineering plasmid is...

  9. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...ii) embedded in a gel, or (iii) attached to a substrate. These in vitro methods allow for a well-controlled... exchanging excitation filters). This avoids substantial changes in excitation power and will help to ...Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire Fluorescent Protein...

  10. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...Nanobodies binding GFP were designed in 2009. Subsequent work showed that nanobodies could be fused to...suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization, Tang et al. tested pairs...soon to allow genome modification only in a small subset of cells? Other proteins may also be utliized as...

  11. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...cell. Once this first order infection occurs, subsequent cycles of viral replication and retrograde transmission...viral replication are toxic. Second, infecting subsequently higher and higher order neurons doesn’t reveal...but the newly released RABV cannot infect any subsequent cells due to lack of G protein on the viral envelope...

  13. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...unusual substrates that other organisms can’t use. Other plasmids offer resistance to toxic substances like...possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the area, freeing up...

  14. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... were exposed to blue light. Cre-Vvd showed substantially improved activity as compared to other recombinase...fluorescent reporter. Find the plasmids here Yongsub Kim’s lab engineered prime editors to expand their...

  15. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...extracted from purified, DNase-treated AAV and submitted for NGS. Raw sequencing data is analyzed to determine...sequences. Recombinase-dependent sequences may be subject to recombinase-independent recombination during...Check out our Viral Vectors Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We Use NGS...

  17. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...check its subcellular location and solubility. If it’s membrane-bound or located in a subcellular compartment...strengths. If that’s not working, consider using a subcellular fractionation kit or a lysing protocol developed...strength Sonication based lysing protocol Subcellular fractional kit or lysing protocol Not enough...

  18. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ... in the depletion of essential genes. In the subsampling analysis of the sgRNAs, Brunello even with only...number genes may mimic viability genes as they are subject to an increase in dsDNA breaks that can cause cell...al. demonstrated that the Calabrese library substantially identified more hits than the SAM library approach...

  19. MXS Chaining

    Type
    Blog Post
    ...generated with MXS-chaining were used to label subcellular structures in live cells. Four individual expression...detection of each fluorophore at the respective subcellular structure (Figure 1). Here, MXS Chaining gave...Excitation/emission maxima Tethering partner Subcellular localization 1 TagBFP 399nm/ 456nm histone ...

  20. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...reporter (Figure 2). The GUS enzyme cleaves a substrate, which will then form a blue precipitate. GUS ...Luciferases are great quantitative reporters and use a substrate to produce bioluminescence. Luciferases usually...fused to a protein of interest to elucidate the subcellular localization of your protein of interest. Find...
Showing: 181 - 200 of 578 results