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Showing: 1 - 20 of 490 results

  1. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ... out Joanne's Reddit AMA Here are 6 steps to submitting a resume that will give you a chance at an interview...interview:  1) Have an excellent resume ready to be submitted The structure and content of a resume can be crucial...the hiring group.  Read here for more on timely submission of a job application. 3) Read job descriptions... not likely know the exact person monitoring submissions (usually a Human Resources representative). Pro...

  2. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...and selected subcellular marker proteins, it’s possible to get an idea about the subcellular structures ...protein function. Getting ready to determine the subcellular localization of your protein of interest The ... vesicles via the secretory pathway to their subcellular or extracellular destination. At the end of their...proteins tagged with FPs to highlight various subcellular structures. Please see Figure 1 for commonly ...inert fluorescent protein fusions to visualize subcellular structures. For example, you might use your fluoresent...particular gene. For subcellular markers in yeast see our plasmid collection from...You can find more plasmids for labeling your subcellular structure of interest in the Allen Institute ...

  4. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...where your plasmid yield is sub-optimal? If you are consistently getting sub-optimal plasmid yields from...your stab from Addgene, streak for single colonies, sub-culture, and prep with a DNA prep kit or your lab's...plasmid yield. Start with a single, fresh colony Sub-culturing directly from a frozen glycerol stock or...

  5. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...Visualization of neuronal sub-cellular structures GFP is limited in its ability to label subcellular structures in...be used to amplify the direct smFP signal within sub-cellular structures. For instance, smFPs have been... be very effective probes for finely visualising sub-cellular structures or low-abundance proteins. When...

  6. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...of local temperature sensing with sub-millisecond resolution and sub-degree temperature differences under...temperature variations and heat transfer at the subcellular level requires a probe with fast kinetics and...

  8. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...repair template strategies activate different HDR sub-pathways like synthesis-dependent strand annealing...DNA repair (with bonus gRNA design resources.) Subscribe to CRISPR updates on our blog to keep current ...

  9. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...natural evolution would produce transposons with sub-maximal efficiency, and this barrier could be overcome...best evoCAST featured 20 mutations in TnsB, the subunit that catalyzes DNA insertion, along with both evolved...

  10. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and... calculate the amount of bound ligand (after subtracting out non-specific binding). Historically, glutathione-S-transferase...to express a foreign protein in bacteria and subsequently purify the fusion protein from a bacterial extract...extracellular domain of the erythropoietin receptor and subsequently evaluated interactions with mutant and wild-...

  11. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...regular group meetings, journal clubs, and focused sub-group meetings to discuss day-to-day progress, ideas...

  12. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...direct your biosensor to specific tissues or cell (sub)types for expression and downstream monitoring. Nervous...

  13. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...For example, calcium in the ER lumen reaches the sub-millimolar range, much higher than in the cytosol...population of cells (e.g., a neuron subtype) and specific subcellular compartments for measurements. All...Localization The calcium concentrations in different subcellular compartments can vary greatly. Additionally, ...changes you will observe in your experiment could be subtle or dramatic. You want to use a calcium sensor that...allosteric rearrangements of the sensor, and (3) subsequent change in fluorescence only occurs at high calcium...sensor to specific tissues or even distinct cell subtypes for efficient expression and downstream monitoring...

  14. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...the desired edit to still occur if the cut site is sub-optimal? As with everything in biology, the answer...little flexibility in the location of the cut and subsequent edit. In these applications, location takes priority...requirement to allow flexibility in gRNA selection and subsequent editing. Identifying PAM-free and -flexible Cas...

  15. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ... genome-wide CRISPR libraries and a selection of sub-libraries targeting specific classes of human genes...

  17. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...source - find a lab within your own neuroscience sub-discipline that has published using the technique... behavior (activation of the circuit promotes subsequent, repeated activation), this is an approximation...behavioral effect but the function of specific subregions within the hippocampus were only recently studied...

  19. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...a Good Conflict Resolver? The first and foremost sub-skill to have here is pretty intuitive: You need ...also be categorized as either “personal” or “substantive”. A personality-based conflict occurs when two...a lab or work environment if left unchecked. Substantive conflict occurs when people or teams who normally...”) when in fact many sources of conflict are substantive (“Person C told Person A one thing and inadvertently...compromise and arbitration might remedy short-term substantive conflicts, but they could also aggravate long-term...

  20. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... biosensors can be imaged at spatial scales from sub-cellular to whole-body in small animals, such as ...intensities in NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps...antigen-dependent fluorescent nanobodies. Nat Methods submitted (2021). Tchekanda, E., Sivanesan, D. & Michnick...
Showing: 1 - 20 of 490 results