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We narrowed to 568 results for: Star

Showing: 181 - 200 of 568 results

  1. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...function due to the use of exon skipping, alternative start codons, and/or the phenomenon of transcriptional...requires a few initial considerations, such as the starting material (DNA-based vector or purified RNA) and...found at Addgene that could serve as an excellent start (#22988, #137884). Both vectors allow the creation...

  2. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...they come in contact with a nutrient source, they start proliferating shortly. Once they are present in ...major source of contamination, the prevention steps start from you. If you are careful enough to provide a...For more tips, check out Addgene's video Getting Started with Tissue Culture below! Mycoplasma removal...

  3. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...Obtaining higher quantities of DNA generally means starting with higher volumes of bacterial culture. Plasmid...sufficient plasmid DNA, you may need to adjust your starting culture conditions rather than your kit.   Do ...recycled materials Sustainable forestry certification Starch-based compostable bags for kit components Recyclable...

  4. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...different depending on the sample and target type. To start the validation process, one must first confirm that...Characterization through Open Science) initiative has now started producing flow cytometry characterization data ... for your flow experiment. We find it useful to start with a screening procedure in easily genetically...

  5. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...interest, and health. While it’s always helpful to start with a literature search to see what others have...considerations you’ll need to be aware of before starting an experiment. Controlling expression As systemic...sensors, CRISPR elements, etc... *These are only starting recommendations; you will still need to experimentally...are a good fit for your experiment, it’s time to start selecting and testing systemic capsids! Figure 2...

  7. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...Titration by qPCR written protocol page. Getting started with tissue culture The cultivation of mammalian...video for some tips and best practices for getting started with tissue cultures. To keep up on all of the...

  8. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...helped scientists around the world quickly get started with COVID-19 research (check out this article ...from 49 countries* and reached 102 countries. We started from 474 plasmid deposits in 2014 which has grown...

  10. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ...16 and 18.” HPV plasmids as positive controls To start her work, Gladys will improve cervical cancer screening...Plasmid Grant to a third round of applicants at the start of 2017 and would like to thank Seeding Labs for...

  11. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ...transition now to being general counsel of a small start-up. For the last 20 years, I was a patent counsel...different jobs. Being general counsel at a small start-up is very interesting. It’s very varied. There’...

  12. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    ...you with your experiments, but a new look. We started the blog in 2013 to connect with the scientific...dropdown menu. These pages are a great place to start if you’re new to the topic. Or, try the search bar...

  14. Addgene's Website Update

    Type
    Blog Post
    ...Starting today, you may notice some changes to our website! Part of sharing science and making access...plasmids from a specific paper. If you’re ready to start a deposit, simply click on “Deposit Materials” and...

  17. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...David Sabatini at the Whitehead Institute and MIT, started with a list of 17 signaling pathways that are frequently...Wood says, and it took them about six months from start to finish. They cloned all the cDNAs into a single...

  19. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ... at our next group meeting and see if we should start celebrating papers and grants too with pizza or ...Choosing a Research Project Advice for Scientists Starting a Lab ...
Showing: 181 - 200 of 568 results