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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11 results for: Star

Showing: 1 - 11 of 11 results

  1. Plan Your Experiment

    Type
    Guide
    ...This page provides a general framework to get you started using CRISPR in your research and setting up CRISPR...understanding of your experimental goal, you are ready to start designing the specific components (Cas enzyme and...aware that there could be downstream translation start sites that could allow for expression of a truncated...elongation. CRISPR Activation : Target the transcription start site. CRISPRa requires dCas9 to be fused to transcriptional...to be within a given range of the transcription start site in order to function effectively. Select gRNAs...

  2. Molecular Biology Reference

    Type
    Guide
    ... GUA, GUG Start AUG* Stop UAG (amber), UGA (opal), UAA (ochre) *AUG is the most common start codon. Alternative... that binds to complementary DNA and acts as a starting point for the DNA polymerase. Thus to replicate...each amino acid. There are 20 amino acids plus a start and stop codon. Below you will find helpful resource...Alternative start codons include CUG in eukaryotes and GUG in prokaryotes. Common Epitope Tags Tag Amino...

  3. Promoters

    Type
    Guide
    ... the start codon and contains the RNA polymerase binding site, TATA box, and transcription start site ...transcription. The most 3' portion (closest to the gene's start codon) of the core promoter is the TSS which is ...upstream) and -35 positions from the transcription start site (TSS). Their equivalent to the eukaryotic TATA...

  4. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Gamma-retroviral vectors tend to prefer transcription start sites, such as promoters and enhancers. Many times...Crise, B., & Burgess, S. M. (2003). Transcription start regions in the human genome are favored targets ...or RSV. 3' LTR in cis Terminates transcription started by 5' LTR by the addition of a polyA tract just...

  5. Addgene's eBook Collection

    Type
    Guide
    ...When you're not sure where to start or need a handy reference, Addgene's eBooks are here to help!... eBook Collection When you're not sure where to start or need a handy reference, Addgene's eBooks are ...

  6. CRISPR Guide

    Type
    Guide
    ...various applications of CRISPR, and help you get started using CRISPR in your own research. CRISPR Basics...Overview of CRISPRi/a Targeting dCas9 to transcription start sites is sufficient to repress transcription by ...recruits Cas3. Cas3 nicks the non-targeting strand and starts degrading the single-stranded DNA, unraveling DNA...

  7. Cloning

    Type
    Guide
    ...This may be due to speed, cost, availability of starting materials or just personal preference. Check out... fragments you want to assemble before you get started. Check out Addgene's website for easy to use MoClo...

  8. Science Guides

    Type
    Guide
    ...Addgene has created the following guides to help you start learning about various scientific concepts related...

  9. Chemogenetics Guide

    Type
    Guide
    ...Planning Your Chemogenetics Experiment If you're starting to plan your chemogenetics experiment, here are...

  10. Adenovirus Guide

    Type
    Guide
    ...transcripts are expressed after DNA replication starts and primarily encode for structural proteins needed...

  11. Lentiviral Vector Guide

    Type
    Guide
    ...or RSV. 3' LTR in cis Terminates transcription started by 5' LTR by the addition of a polyA tract just...
Showing: 1 - 11 of 11 results