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We narrowed to 616 results for: TIL

Showing: 181 - 200 of 616 results

  1. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...fluorophores, then labeling with the other, you can still record the signal separately for each target.   ...., 2020 multiplexed over 65 targets!) IF is a versatile method with endless possibilities, and there are...Hernandez, J. M., & Germain, R. N. (2020). IBEX: A versatile multiplex optical imaging approach for deep phenotyping...

  2. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ... ways to engineer an antibody, thanks to the versatility of the basic antibody structure (Figure 1). Antibodies...’ll typically go through this cycle many times, until you achieve whatever goal you’re aiming for. When...development methods. Despite recent advances, there is still always room to improve, and scientists will continue...

  3. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...normality (or lack thereof). Alternatively, a Quantile vs. Quantile plot can determine if your data deviates...data in ascending numerical value relative to quantiles derived from a theoretical distribution. If your...

  4. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...myths that abortions cause breast cancer and infertility or to argue against unnecessary clinic regulations...had been so well-publicized, we might encounter hostile anti-science activists. However, I must say that...in Vancouver, BC, although I wasn’t sure I’d go until several hours before it started. It was great to...

  5. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... the wrong address in a different city, you can still develop a successful FRET-based assay since energy... Scientific). These reagents are nonfluorescent until they bind a 6-residue tetracysteine tag (sequence...that can be inserted into the target protein. The utility of these reagents is that binding is covalent, ...

  7. Our New Antibody Mascot is....

    Type
    Blog Post
    ...vote on @addgene's Twitter poll. Voting is open until Feb. 22, 2022, and we'll unveil the winning name...if it’s also a great pun.) Submissions are open until Feb. 3, 2022.  The Addgenies will choose up to four...

  8. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ...pic.twitter.com/mk44J4Gcj9 — Dave W will get vaccinated and still wear a mask. (@GermJuggler) February 13, 2021 Westenberg...over the second quadrant. The process continues until all quadrants are used, hopefully revealing single...

  9. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...our viral service in late 2016 and requests are still pouring in! While our lentiviral service is going... they come from and what do they mean? AAV: A Versatile Tool for Gene Expression in Mammals Subscribe ...

  10. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...of ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA...

  12. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ... leave their foraging trail. They climb upwards until they reach a point where the temperature and humidity...enters fish through its gills. It attaches there until they mature and changes sex from male to female....

  14. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...seeking a first job after the academic bench. Utilizing existing relationships as part of the job application...you a much better chance at an interview –but you still need to nail the job yourself.   See the links in...

  15. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...been there to answer your plasmid questions. But until now, you’ve always had to call the United States...and depositing (all while our US counterparts are still sleeping). Plasmid sharing in Europe: Addgene’s ...

  16. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...antibody fragment was exciting, and it wasn’t long until the field was off and running with camelid-derived...So thank you to the camelids who inspired this versatile family of tools, the applications they have already...

  18. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...cleaved off from the fusion protein while it is still bound to the glutathione resin, eliminating the ... proteins in mammals. Mammalian purification is still possible but requires optimization and often more...

  19. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...process known as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that...driving particular cells or responses. And there are still antibody isotypes and Fc receptors that we don’t...

  20. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...endogenous function of the gene as a result but will still allow for expression pattern monitoring. Enhancer...insertion into an exon, like promoter traps, it will still likely disrupt the gene it monitors due to transcriptional...
Showing: 181 - 200 of 616 results