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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 34 results for: TIL

Showing: 1 - 20 of 34 results

  1. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...by PCR, predictability of loss-of-function, and utility for the study of non-coding elements. This approach...markers for selection 1 . Other constructs may be utilized, such as pX458 (Addgene plasmid ID 48138) or pX459...For small deletions (as the “deletion band” may still amplify), resolve agarose gel to determine if size...sequencing primer; other flanking primers may be utilized. Choose a sequence-verified colony and inoculate...extraction, any method for genomic DNA isolation may be utilized to be able to perform PCR in step 6.3 . Assemble...

  2. Bacterial Expression Systems

    Type
    Collection
    ...pCri System (Addgene #1000000058) plasmids can be utilized for heterologous cytoplasmic and periplasmic protein... protein expression in E. coli , Bacillus subtilis , and Pichia pastoris . The EcoFlex MoClo Toolkit (...luminescence (lux operon) Gram-negative bacteria Attila Karsi 14080 pAKlux2 Promoter activity Luminescence...Luminescence (lux operon) Gram-negative bacteria Attila Karsi 14076 pAKgfp1 Promoter activity Fluorescence (GFPmut3a...(GFPmut3a) Gram-negative bacteria Attila Karsi 18855 FLIParaF.Ec-250n Arabinose FRET fluorescence (CFP...

  3. COVID-19 Resources

    Type
    Collection
    ... . Feng Zhang's lab at the Broad Institute has utilized the natural RNAse activity of the Cas13 protein...Institute has published information and protocols for utilizing SHERLOCK to detect coronavirus - Enabling coronavirus... 2020.04.20.052159 . Jennifer Doudna's lab has utilized Cas12a’s non-specific ssDNA degradation to develop...Biosciences has published information and protocols for utilizing DETECTR to detect coronavirus - A protocol for...

  4. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...networks involved in retinal development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress...listed here are provided as empty backbones that utilize Golden Gate cloning to insert the desired guide...guides can be expressed from the same plasmid by utilizing one of the dual guide shuttle plasmids to insert...

  5. Plan Your Experiment

    Type
    Collection
    ...cell type. Figure 2: Different delivery systems utilized for CRISPR/Cas gene editing. This image summarizes...contains a selection marker. Plasmid delivery is versatile thanks to the wide range of options for the Cas...also transient, as the RNPs will only be active until they are degraded. This method is helpful in cells...will ultimately depend on which CRISPR method you utilized. If your edit affects genome sequence, your edited...

  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...easy multiplexing/scaling. Prokaryotes have long utilized CRISPR as a powerful defensive strategy against...safeguard against bacteriophages was not elucidated until 2007. Scientists hypothesized that prokaryotes used...CRISPR as part of an adaptive immune system - utilizing various CRISPR-associated (Cas) genes to not only...

  7. Optogenetics Plasmid Collection

    Type
    Collection
    ...proteins can be expressed in specific neurons, often utilizing viral expression systems, to activate or deactivate...can also be controlled by light, generally by utilizing a light sensitive protein from cryptochromes, ...

  8. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...abundant in nature, Class 1 systems are largely underutilized compared to their Class 2 counterparts like ... and Cascade on one single plasmid, though many still separate the individual Cas components. Figure 1...

  9. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...HindIII No Tilmann Weber 172717 pnsgRNA Bacteria J23119 (BBa_J23119) nicking sgRNA No Tilmann Weber 173220...

  10. Immunology Research Plasmids and Resources

    Type
    Collection
    ...Name Synonyms TLR1 toll-like receptor 1 TIL, CD281, rsc786, TIL. LPRS5 TLR10 toll-like receptor 10 CD290...glycosylation-inhibiting factor) GIF, GLIF, MMIF MLN motilin MGC138519 MLNR motilin receptor GPR38, MTLR1 MPL myeloproliferative...containing 1 ANUP, ARS, ArsB, LY6LS, MDM SORT1 sortilin 1 Gp95, NT3 SPP1 secreted phosphoprotein 1 BNSP..., pseudogene(TLR12P) - TLR2 toll-like receptor 2 TIL4, CD282 TLR3 toll-like receptor 3 CD283, IIAE2 TLR4...CD284, TLR-4, ARMD10 TLR5 toll-like receptor 5 SLE1, TIL3, SLEB1, MELIOS TLR6 toll-like receptor 6 CD286 TLR7...

  11. TALEN Guide

    Type
    Collection
    ...but they still have potential targetable sites approximately every 35bp. Researchers are still determining...

  12. CRISPR Guide

    Type
    Collection
    ...close enough to cause a DSB. The nickase system is utilized in many CRISPR systems to achieve specific genetic..., a large portion of the Cas9-induced DSBs will still be repaired via NHEJ. The resulting population of... be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome painting requires delivery...CAPTURE system, developed by the Jian Xu lab , utilizes biotin tagging of dCas9 by fusing a biotin acceptor...

  13. Neurodegeneration Research Collection

    Type
    Collection
    ...years from symptom onset. Disease mechanisms are still poorly understood but several genes and mapped loci...dopaminergic (mesDA) neurons using gene knockout . Maimaitili et al. Nat Commun. 2023 Dec 5. See More Antibodies...

  14. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ..."flanked by loxP"), will permit gene expression until Cre is present, at which time the gene will be disrupted...reading frame), this system allows scientists to utilize recombination elements such as Cre to turn off ...

  15. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...161769 Inhibition Yeast Ingolia NA ~6 44,580 KSHV Tiling Library 180272 Knockout KSHV Glaunsinger 3rd NA...Gonatopoulos-Pournatzis 3rd Varies 95,893 CRASP-Seq BE Tiling Library 232070 Base Editing Human Gonatopoulos-...

  16. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...with the endogenous tRNA-processing system Plant Utilizes the tRNA processing system to reduce size and ...1 and U6:3 promoters. Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise...

  17. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Twinkle-APEX2-V5 TWNK CMV Spinocerebellar ataxia, infantile onset Alice Ting 131480 pORANGE GFP-CACNA1A KI...Halo_C_allele PLA2G6 Halo Dystonia-parkinsonism, Infantile neuroaxonal dystrophy Michael Ward 178167 RNF216...SUMO-Twinkle 43-372 TWNK His T7 Spinocerebellar ataxia, infantile onset Smita Patel 205055 SUMO-Twinkle 360-684 ...684 TWNK His T7 Spinocerebellar ataxia, infantile onset Smita Patel 205056 SUMO-Twinkle 43-684 TWNK His ...His Spinocerebellar ataxia, infantile onset Smita Patel 205635 pKM-U6-reRNA-mmDNMT1 DNMT1 U6 Hereditary ...
Showing: 1 - 20 of 34 results