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We narrowed to 617 results for: TIL;

Showing: 181 - 200 of 617 results

  1. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...normality (or lack thereof). Alternatively, a Quantile vs. Quantile plot can determine if your data deviates...data in ascending numerical value relative to quantiles derived from a theoretical distribution. If your...

  2. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...myths that abortions cause breast cancer and infertility or to argue against unnecessary clinic regulations...had been so well-publicized, we might encounter hostile anti-science activists. However, I must say that...in Vancouver, BC, although I wasn’t sure I’d go until several hours before it started. It was great to...

  3. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... the wrong address in a different city, you can still develop a successful FRET-based assay since energy... Scientific). These reagents are nonfluorescent until they bind a 6-residue tetracysteine tag (sequence...that can be inserted into the target protein. The utility of these reagents is that binding is covalent, ...

  5. Our New Antibody Mascot is....

    Type
    Blog Post
    ...vote on @addgene's Twitter poll. Voting is open until Feb. 22, 2022, and we'll unveil the winning name...if it’s also a great pun.) Submissions are open until Feb. 3, 2022.  The Addgenies will choose up to four...

  6. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ...pic.twitter.com/mk44J4Gcj9 — Dave W will get vaccinated and still wear a mask. (@GermJuggler) February 13, 2021 Westenberg...over the second quadrant. The process continues until all quadrants are used, hopefully revealing single...

  7. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...of ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA...

  8. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...our viral service in late 2016 and requests are still pouring in! While our lentiviral service is going... they come from and what do they mean? AAV: A Versatile Tool for Gene Expression in Mammals Subscribe ...

  9. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ... leave their foraging trail. They climb upwards until they reach a point where the temperature and humidity...enters fish through its gills. It attaches there until they mature and changes sex from male to female....

  12. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...seeking a first job after the academic bench. Utilizing existing relationships as part of the job application...you a much better chance at an interview –but you still need to nail the job yourself.   See the links in...

  13. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...been there to answer your plasmid questions. But until now, you’ve always had to call the United States...and depositing (all while our US counterparts are still sleeping). Plasmid sharing in Europe: Addgene’s ...

  14. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...activity is also drawn out as drugs can remain present until cleared by the body. Administering the drug once...Designer Drugs): Chemogenetic Tools with Therapeutic Utility. Annual Review of Pharmacology and Toxicology 55...

  16. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...cleaved off from the fusion protein while it is still bound to the glutathione resin, eliminating the ... proteins in mammals. Mammalian purification is still possible but requires optimization and often more...

  17. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...process known as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that...driving particular cells or responses. And there are still antibody isotypes and Fc receptors that we don’t...

  18. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...endogenous function of the gene as a result but will still allow for expression pattern monitoring. Enhancer...insertion into an exon, like promoter traps, it will still likely disrupt the gene it monitors due to transcriptional...

  20. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...but not thermophilic (high heat loving) bacteria. Until recently the only available Cas9 proteins were isolated... GeoCas9 RNPs to be incubated at high temps but still maintain activity following heat treatment. The ...
Showing: 181 - 200 of 617 results