...recombine DNA? There are many methods that have been utilized over the years to move around pieces of DNA. Oftentimes...in the DNA. In restriction cloning, scientists utilize specific restriction enzymes to cut dsDNA of interest...reducing the amount of time required for cloning. To utilize this approach, the fragment of DNA that you would...pairs adenine (A) and thymine (T). TOPO® cloning utilizes the Taq polymerase which naturally leaves a single...traditional restriction enzyme cloning. Scientists have utilized this approach to create compatible custom overhangs...polymerase will continue to function as an exonuclease until a base is exposed on the single strand overhang ... 100kb) compared to other cloning methods that utilize bacteria where it becomes progressively more difficult...

...for light-induced cAMP modulation. Utilizes BLUF (blue light utilizing FAD) domains. 441 Phytochromes BphP1...technologies developed for optogenetics research utilize light to detect, measure, and control molecular...domains from two different ChRs. These ChR variants still function as light-gated, cation channels resulting...used, there is an incubation time (days to weeks) until peak expression of the opsin is reached. This approach...figure above, this light induced binding can be utilized to control the localization of a protein of interest...

...cross the blood-brain barrier in rats, and the utility of DREADDs in experiments in this species may be... as laser or LED illumination), making LMOs a versatile option. Coupling brighter bioluminescent modules...reporter ensures that the reporter remains silent until a cell or tissue-specific Cre is provided. Want ...genetically-encoded bioluminescent opsins for versatile, scalable, and hardware-independent optogenetic...

...transfer plasmid. 2nd generation lentiviral plasmids utilize the viral LTR promoter for gene expression, whereas...whereas 3rd-generation transfer plasmids utilize a hybrid LTR promoter (more information on this below)...basically shuffling restriction sites between vectors until the gene of interest is flanked by sites compatible...

... DNA sequencing of the plasmid. Primers can be utilized to verify the sequence of the insert or other ...genetic elements. Types of Plasmids Plasmids are versitile and can be used in many different ways by scientists..., like Sanger, is based on DNA replication and utilizes modified fluorescently tagged nucleotides. During...

...close enough to cause a DSB. The nickase system is utilized in many CRISPR systems to achieve specific genetic..., a large portion of the Cas9-induced DSBs will still be repaired via NHEJ. The resulting population of... be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome painting requires delivery...CAPTURE system, developed by the Jian Xu lab , utilizes biotin tagging of dCas9 by fusing a biotin acceptor...

... simpler than eukaryotic transcription bacteria still have complex systems of gene regulation, like operons...opposite way. The operon is normally transcribed until a repressor/corepressor binds to the operator preventing...

...technologies developed for optogenetics research utilize light to detect, measure, and control molecular...

...using helper-free packaging cell lines This method utilizes a packaging cell line that stably expresses Gag-Pol...

... sequencing primer" and "3' sequencing primer". Still not sure what primer you need? Email us at help@...

...desired edit, as a large percentage of DSBs are still repaired by NHEJ. How do you determine that your...

...bind to a protein is powerful, but in order to utilize it in the lab, researchers must be able to detect...