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We narrowed to 501 results for: Trac;

Showing: 181 - 200 of 501 results

  1. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...scientists are paid above this threshold or for tracking the hours that these scientists work and paying...across academia). Increased postdoc wages, overtime tracking? What are institutions doing? Institutions are...their salary raised 101% if hours are not to be tracked. University of Buffalo and Ohio State both have...

  2. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...General expression mRNA Tetracycline response element promoter Inducible with Tetracyline or its derivatives...promoter with low basal activity and several tetracycline operators. Transcription can be turned on or...

  3. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...useful to avoid such procrastination: Remove distractions: Probably the easiest way to do this is to leave...internet and all it’s enticements are far too distracting. (As a side note, I’d recommend the book, “Bored...more fantastic reasons to remove yourself from distractions every once in awhile.) Reframe the task in a...

  4. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...thrive in laboratory settings. One of its most attractive features is its stability and easily obtainable...ability to regenerate, the axolotl has several attractive characteristics that make them an ideal model...for human disease research. One of their most attractive features are their eggs. Frog eggs are large,...

  5. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... signal is boosted and enables single molecule tracking in living cells. The HA frankenbody is an HA tag...expressed on T cells to make CAR T cells. The extracellular scFv portion of the receptors recognizes an ...an antigen of interest while the intracellular T cell receptor portion of the CAR facilitates signal transduction...

  6. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...like lamellipodia or filopodia formation, cell contraction, or other morphological changes, depending on...tools to control formation of lamellipodia and contractile stress fibers, and recently developed a full ...selection in mammalian cells used here could be an attractive strategy for engineering other enzymes. Conclusion...

  7. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...therapeutics, which is also gaining a lot of new traction lately (1, 2), depends on knowledge of the structure...storage. After overnight growth, scrape up, pool and extract plasmid DNA directly from the colonies. Transform...solution containing your favourite bacterial protein extraction reagent (BPER from Thermo works well) with some...

  8. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...microglia. This property makes the lentivirus an attractive choice for a gene transfer vector. Lentiviral...used inducible lentiviral vector system is the tetracycline (Tet)-regulated system. You can choose either...promoter in your transfer vector. In the absence of tetracycline or a derivative such as Doxycycline (Dox), the...

  9. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...culture through various sources that are difficult to trace. These include the laboratory personnel, the serum...mycoplasmas, contamination by other bacteria or extracellular fluorescent signals produced by these bacteria...fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline, etc. are available for mycoplasma removal from...

  10. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...the sensitive FLEx-split TVA receptor used for tracing neural pathways was 8.5 x 10e10 GC/mL. The lot ...Adeno-Associated Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678. https://doi.org...Jin L, Lea NE, Wickersham IR (2020) Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations...

  12. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    .... Other dyes may prove challenging to stain intracellular compartments in in vivo model systems, so extra...green, red, and far-red dyes is important for intracellular fluorescence live imaging ​​(Hayashi-Takanaka...fission, fusion, biogenesis, and mitophagy, can be tracked using dyes like Rhodamine 123 (R123) (Liu et al...

  13. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...normal kit protocols.  Yeast plasmids – If you’re extracting plasmids from yeast, instead of bacteria, a few...kits — there are many more kits out there for extracting DNA from tissues, blood, virus, or other samples...samples, for PCR and gel cleanup, and for extracting RNA! (That’s a blog for another time, however!) Where...

  15. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...barcodes and scanners to keep track of reagents in your lab. With barcode tracking, you will always be able...
Showing: 181 - 200 of 501 results