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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 44 results for: Trac;

Showing: 1 - 20 of 44 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ... Plasmid Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression...Stem Cell Research Plasmids Collection Tetracycline Off Tetracycline On Experimental Considerations Tet Plasmids...shown. Tetracycline Off (Tet-Off) The first major advance was the Tet-Off system. A tetracycline-controlled...presence of tetracycline, tTA unbinds from the TRE, resulting in reduced gene expression. Tetracycline Off is...binds the TRE in the presence of tetracycline. A new reverse tetracycline-controlled transactivator (rtTA... absence of tetracycline, rtTA is unable to bind the TRE. In the presence of tetracycline, rtTA binds ...applications. Use of Tetracycline or a Derivative Doxycycline, a derivative of tetracycline, is a preferred...

  2. AAV for Neuronal Tracing

    Type
    Collection
    ...Monosynaptic Neuronal Tracing Viral Vector Packaging Service: Tools for Monosynaptic Neuronal Tracing These AAV encode...encoding tools for monosynpatic retrograde neuronal tracing, to be used with modified rabies virus. Viral...encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with modified rabies virus...virus to perform retrograde neuronal tracing. Note : Addgene does not distribute rabies virus. AAV Vectors...Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-...Introduction to Rabies Virus-based Monosynaptic Tracing The core idea of the system is to infect the cell... population of interest with a deletion-mutant tracing virus that lacks the ability to infect neighboring...

  3. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...ATP (extracellular) ecAT3.10 extracellular ATP sensor (cell surface targeted) Imaging extracellular ATP...Yasushi Okada ATP (extracellular) GRAB_ATP1.0/ATP1.0mut biosensor for extracellular ATP (Constitutive ...Eckhard Boles pH Imaging intracellular pH in live cells (pHRed) Imaging Intracellular pH in Live Cells with...(e.g. in vitro vs. in vivo, cytoplasmic vs. extracellular vs. organelle-localized). Browse or search the...lifetime-based biosensor for quantitative imaging of intracellular calcium. Nat Commun. 2021 Dec 9;12(1):7159. ... encoded fluorescent indicators for imaging intracellular potassium ion concentration. Commun Biol. 2019...Genetically encoded FRET sensors to monitor intracellular Zn(2+) homeostasis. Nat Methods. 2009 Oct;6(...

  4. Bacterial Expression Systems

    Type
    Collection
    ...proteins in high yields. Proteins can then be extracted from the culture and purified through affinity...bacterial protein contamination and facilitates extraction. Browse our most popular plasmids for protein...Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline (aTc) Escherichia coli Stanley Qi 11518 pDest...coli Andreas Moeglich 68940 pRMC2 Pxyl/TetO Anhydrotetracycline (aTc) Staphylococcus aureus Tim Foster 36267...glutamicum Timothy Lu 17972 pSE100 Pmyc1/TetO Anhydrotetracycline (aTc) Escherichia coli , Mycobacterium tuberculosis...tuberculosis Sabine Ehrt 44561 pST-KT Pmyc1/TetO Anhydrotetracycline (aTc) Escherichia coli , Mycobacterium tuberculosis...extorquens Christopher Marx 44448 pLC291 pR/TetO Anhydrotetracycline (aTc) Methylobacterium extorquens Christopher...

  5. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... μl of DNA extraction solution. NOTE: Generally ~100,000 cells are used for DNA extraction, although a...loading this video, please try viewing on YouTube . Abstract The prokaryotic clustered regularly interspaced...following program: 65 °C for 6 min, 98 °C for 2 min to extract gDNA. Measure the DNA concentration. NOTE: While...and 6.2 recommend an efficient method for DNA extraction, any method for genomic DNA isolation may be ...flicking the PCR plate over a sink. Add 50 μl of DNA extraction solution per well and resuspend. Continue to ... cells to grow. Aspirate media. Add 50 μl DNA extraction solution per well, resuspend and transfer to ...to 96-well PCR plate. Continue to step 7.3 . Extract the gDNA from clones. Run sample in thermocycler: ...

  6. Adenovirus Plasmids

    Type
    Collection
    ... promoter Vogelstein 16404 pAdTrack Shuttle For production of GFP-trackable viruses containing transgene...promoter Vogelstein 16405 pAdTrack-CMV Shuttle For production of GFP-trackable viruses containing transgene...additional plasmids Zhang 50957 RedTrackCMV Shuttle For production of mRFP-trackable viruses containing transgene...

  7. AAV Molecular Tools

    Type
    Collection
    ...Overexpression Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools...tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression...Viral Vector Packaging Service: Molecular Tools Tetracycline Transactivators Affinity Purification Neurophysiology...Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression...

  8. Promega Plasmid Collection

    Type
    Collection
    ...NanoLuc fusions are useful for both intracellular and extracellular applications where you want minimal...HaloTag, SmBiT, and LgBiT, useful for detecting and tracking key drug target proteins. Depositor...analysis and protein fusion vectors for detecting and tracking proteins using tags such as NanoLuc, HaloTag, ...signaling pathways, investigating RNA regulation, tracking protein degradation, analyzing CRISPR-mediated...measured by competitive displacement of a NanoBRET tracer, reversibly bound to a NanoLuc luciferase-fusion...

  9. Zebrafish Plasmid Collection

    Type
    Collection
    ...- Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular systems typically relies...labeling with fluorescent proteins to visualize and track clones of cells over time. Examples of useful tools...Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration - Periklis ...Lab Multibow: digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow - Albert Pan and Alex...fluorescent labeling in zebrafish Whole organism lineage tracing by combinatorial and cumulative genome editing ...

  10. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... Anti-Pan-Neurofascin (extracellular) [A12/18R] Pan-Neurofascin (extracellular) Rat Mouse IgG2a 177439...Anti-Kv1.4 K+ channel (extracellular) [L71/5R] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 177489...Mouse IgG2a 177564 Anti-ADAM22 (extracellular) [N57/2R] ADAM22 (extracellular) Mouse Mouse IgG2a 177565 Anti-GluN2B...Anti-Kv1.4 K+ channel (extracellular) [L71/22] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 190292...-Neurofascin (extracellular) scFv [A12/18] A12/18 scFv 2t Pan-Neurofascin (extracellular) Rat Mouse 190486...Kv1.4 K+ channel (extracellular) scFv [L71/5] L71/5 scFv Kv1.4 K+ channel (extracellular) Rat Mouse 190518... Mouse 190537 LRP4 (extracellular) scFv [N207/27] N207/27 scFv LRP4 (extracellular) Mouse Mouse 190538...

  11. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...trans-activating crRNA (tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps recruit RNAse...together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual...individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks out the DNA sequence complementary...cleaves both strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex unbinds after the double-strand break...

  12. Antibody Guide

    Type
    Collection
    ...samples to extract proteins. Homogenize (tissues only), lyse, and then collect protein extract by centrifugation...and therefore should be kept at 4 °C. Record and track lot numbers for all antibodies used in the lab. ...application Process tissue or cell culture samples to extract proteins. Homogenize (tissues only) and lyse. Cross-link...In IHC, tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule...enzyme. In ICC, cells have most or all of their extracellular matrix removed. Again, the signaling molecule...

  13. Luciferase Plasmid Collection

    Type
    Collection
    ...be lysed or the substrate must be delivered intracellularly to measure luminescence. Gaussia luciferase...membrane-anchored version useful for labeling extracellular vesicles. LumiScarlet : A fusion of LumiLuc ...serve as sensors of proteome stress. pHLuc : An extracellular pH sensor consisting of a pH-sensitive GFP (...OFP (Antares), and Nanoluc®. CalFlux VTN : An intracellular calcium sensor created by fusing NanoLuc® and...

  14. Cancer Research Plasmids and Resources

    Type
    Collection
    ...mutations. Resources New Tool for Lineage Tracing: The ClonTracer Library (Frank Stegmeier) Tackling Cancers...cDNAs for studying oncogenic signaling pathways. ClonTracer Library : Barcoding library from the Stegmeier...

  15. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Dorus Gadella 58473 pEGFP-C1 F-tractin-EGFP Actin Filaments F-tractin EGFP Dyche Mullins 41147 pEGFP ...Caveolae Cav1 mCherry Ari Helenius 37533 cfSGFP2-N Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1... in secreting proteins from the cell to the extracellular environment. Proteins are first translated at...

  16. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Optogenetics Plasmid Collection Monosynaptic Neuronal Tracing CRISPR Plasmids and Resources Site-specific recombinase...only activated in the presence of tamoxifen. A tetracycline-regulated or other drug-inducible approach may...Reporter plasmids can also be useful for lineage tracing, evaluating infection/transfection efficiency, ...

  17. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Find more Tet-inducible empty backbones on our Tetracycline (Tet) Inducible Expression page Bacteria Lac..., secretion, and display of proteins on the extracellular surface of S. cerevisiae cells His-tagged versions...but do NOT integrate into host genome pAdTrack-CMV - Shuttle vector for transgene...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ...CRISPR RNA (crRNA) and the trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also... cassettes to express the CRISPR RNA array and tracrRNA separately (Cong et al ., Science 2013). Additionally...or SpCas9n, D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression cassettes...

  19. CRISPR Guide

    Type
    Collection
    ...dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and RNA tracking in living...editing can specify a single mutation, even within a tract of identical bases. Browse Plasmids: Prime Edit ...proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA...synthetic fusion of the endogenous bacterial crRNA and tracrRNA that provides both targeting specificity and scaffolding...

  20. AAV Viral Preps

    Type
    Collection
    ...Spagehetti Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell...Cell Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples...
Showing: 1 - 20 of 44 results