We narrowed to 760 results for: Vars

...templates. Cas9 variants PE6e–g often had even higher editing efficiencies but varied depending on the...vivo. Incorporating reverse transcriptase (RT) variants, PE6a and PE6b are 500–800 bp smaller than previous...PE6d are optimized for dual-AAV systems. These variants use different RT enzymes and performed large edits...edit.   Figure 1: Choosing the best PE6 variant for your application. Image reused form Doman and... DIRECTED can increase on-target delivery with various delivery modalities and has the potential to reduce...

...The infectious titer of a viral vector stock can vary based on transduction target and can also be altered...Stoilova-McPhie, Center for Nanoscale Systems, Harvard University.    Which titer do people usually...quantified with a different primer pair. Ct values vary based on the amount of starting material in the ...particle. Finally, the precision of the assay can vary up to two fold for the final titer value, which ... same PCR twice can give titer measurements that vary up to two-fold. How can we get more reliable AAV...expression analysis with low abundant targets: from variable nonsense to publication quality data." Scientific...

...recombine independently, cells may acquire one of a variety of colors when a combination of XFP are expressed...systems, many based on Brainbow, now exist for a variety of species, cell types, and research applications...inversion of two genes aligned head-to-head. Another variation, Brainbow 2.1, uses Cre to trigger the inversion...similar systems have been developed for tracking various cell types and organisms and for improving the ...developed from the Brainbow 1.0-L construct, a variant of Brainbow 1.0 that contains RFP, CFP, and YFP...labeling systems allow single cell dynamics in a variety of organisms to be observed in more detail than...

...Researcher at Harvard Medical School, former dean of the faculty of Medicine at Harvard University 29:...Last Wednesday we worked with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility... talk and at the minisymposium. You can find a variety of additional resources and information about organizations...Science) Edward J. Hall (Professor of Philosophy at Harvard University) Tony Cijsouw (Neuroscience postdoc ...points out in his talk (1:24 - 29:31), there are a variety of ways of defining scientific reproducibility....

...made it easier to create knockout alleles in a variety of species, including the standard laboratory mouse...see deletions of the region between the gRNAs. A variety of other undesired outcomes, including one loxP...Easi-CRISPR for simple knock-ins and floxed models varies from 8.5-100% of pups born, with typical efficiencies...30-60%. Quadros et al. attribute much of this variation to differences in guide RNA efficiency. For example...correctly edited founder. Figure 6: Diagram of ovary and oviduct anatomy with the equipment used for ...female mice using a small incision that exposes the ovaries and oviducts. After solution injection into the...

...served as the Chief Human Resource Officer for Harvard Medical School. The addition of Cicalese to the...member. When I was working in Human Resources at Harvard Medical School, Joanna was my colleague. She mentioned... committee because I had recently retired from Harvard, and it let me keep my toe in the water of HR work...understand the science but my prior HR experience at Harvard Medical School means I understand the needs of ...served as the Chief Human Resources Officer for Harvard Medical School where she brought leadership teams...

...Matthew Canver of Boston Children’s Hospital and Harvard Medical School. Addgene is proud to present a video...paired double-strand break (DSB) approach at a variety of loci. We were pleasantly surprised by the efficiency...investigator at Boston Children’s Hospital and Harvard Medical School. His lab studies the molecular genetics...bauerlab.org.     Matthew Canver is an MD/PhD student at Harvard Medical School in the laboratories of Stuart Orkin...erythroid enhancer of BCL11A subject to genetic variation determines fetal hemoglobin level. Science 2013...

...relies on kinesin. Different rates of speed for various forms of retrograde and anterograde transport suggest...visual, and auditory neuronal circuitry in vivo. A variant of RABV has been developed to only label a neuronal...proteins VP1, VP2, and VP3. To date several hundred variants, or serotypes, have been found naturally or have...Janelia Research Campus also developed a new AAV variant, rAAV2-retro, that migrates retrogradely at higher...toxicity, higher specificity and reduced technical variability compared to chemical tracers. rAAV2-retro also...viral preparations of AAV2-retro vectors with a variety of transgene/promoter combinations. Check out our...D.V., and Karpova, A.Y. (2016). A designer AAV variant permits efficient retrograde access to projection...

...Global Head Muscle Diseases at Novartis, opened with a discussion of the Novartis industry postdoctoral program...postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School and a long list of supporters from...Lahav, Associate Professor of Systems Biology at Harvard Medical School, titled her presentation “How to...Marc W. Kirschner, Shirley Tilghmanc and Harold Varmus     ...

...the genomes of many different fish species in a variety of non-functional forms and is believed to be millions...system was synthesized by fusing and modifying various extinct transposases. Improvements have been made... extinct transposon sequences and then testing various combinations of changes. The basic functional version...Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts...inducible expression vectors were developed into 16 variants with different selection markers (blasticidin,...

...developed an adaptive immune system that utilized various cas genes not only to store a record of invading... prokaryotic species suggest the presence of a variety of undiscovered viral anti-CRISPR genes. These ...specificity, orthogonality, and multiplexibility in various species and describe an assortment of applications...specific, programmable platform well-suited for various innovative biological and translative technologies... enabling access to multiple plasmids used for various platform applications. For more information on ...

...and heavy chains made up of variable regions and constant regions. Variable regions are unique to each ...Figure 1: In the isotype conversion process the variable regions of a parental antibody (blue, rat) are...resulting chimeric antibody would have the original variable regions from the parental rat antibody but the... tools. In the case of isotype conversion, the variable regions of a specific antibody are cloned into...

...research to explore a variety of phenomena, and so too can it be used to teach a variety of science concepts...Brassicaceae family, Arabidopsis is related to a variety of food crops such as broccoli, cabbage and kale...concepts. From inheritance and variation, to growth and development, and response to environment - ABRC has... conduct the experiments outlined in ABRC’s kits vary by module. As a general guideline, experiments that...

...chemical fluorophores can still be used in a wide variety of experiments. Read more to find chemical fluorophores...a postdoctoral researcher in the Silver Lab at Harvard University, describes R-bodies, “force generating...commonly used to deliver a genes of interest to a variety of cell types in-vivo. These are widely used by...respectively. Didem Goz Aytruk from Connie Cepko’s Lab at Harvard Medical School describes how AAVs function and ...proteins, dCas9, and a modified gRNA to direct a variety of customizable functions to your DNA sequence ...

... Although we only walk one path at a time, the variety of paths in life is mind-boggling. Unlike a mountain... editor In my role as an editor at Science, my various interests come together as useful and productive...or policy makers. The term editor covers a wide variety of careers and jobs. At Science, which is published...Fellows Program). Science journalism programs at various universities range from week-long workshops to ...engagement through science communication with a variety of audiences. She focuses at Science on developmental...

...behaviors often fall short with the generation of various flux imbalances. The conventional approach has ...engineering have been limited to the overexpression of various metabolites in Escherichia coli or Saccharomyces...modular synthetic RNA scaffolds that can recruit various transcriptional regulators (Zalatan et al. 2015...RNA scaffolds. These RNA scaffolds can recruit various transcriptional regulators to alter gene expression...metabolic flux. The dCas9 protein and orthogonal variants can be used as regulatory ON/OFF master switches...

...cancer cells but are directed not to through these various signals. Over-production of vascular endothelial...made to use whole cell therapeutics consisting of various types of immune cells. Dendritic cells (DCs) are...Check out this review for an in-depth look at a variety of up and coming therapies. Future directions ...Cell heterogeneity Tumors are composed of a wide variety of cell types. This cell heterogeneity is an important...of the genotypic and phenotypic changes in the various types of cancer will fuel the development of immunotherapeutics..."Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts...

... build single- and multi-gene constructs for a variety of applications. MoClo tools can be used in bacterial...Keep in mind that this technology is useful for a variety of purposes beyond what we discuss. If you can ... multi-TU constructs (Moore et al., 2016). The variety of constitutive and inducible promoters, linkers...Beyond sgRNAs, MoClo can also be used to test a variety of Cas enzymes with different PAM requirements ... MK system, many of the parts can be used in a variety of organisms, saving time and money for labs with...the Modular Cloning System.” PLOS ONE, edited by Alvaro Galli, vol. 13, no. 5, Public Library of Science...

...blogger Jon Chow, an immunology PhD student at Harvard University. In this second post in our quick guide...background. In other cases, YFG or one of its mutant variants will need to be overexpressed or knocked down....Stock Center has fly lines expressing Gal4 under a variety of promoters resulting in tissue specific expression... You can quickly mate your UAS-YFG line with a variety of Gal4 lines and study gene expression in many...Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has had nearly a decade of experience...

...sequences (colored rectangles) are cloned into various plasmids using oligonucleotides. These plasmids...depending on which method you use, the procedure will vary slightly.   Two Golden Gate options that are... with the Gersbach lab plasmids, multiple Cas9 variants are available: wt humanized Cas9, D10A nickase...clone your own entry vectors containing other Cas9 variants to use with the MuLE system. Multiplexing from...into one of 12 pYLsGRNA plasmids, which contain various promoters and reporters, and subsequently inserted...Topic Page Get Advice on Choosing The Proper Cas9 Variant for Your Experiment Learn About Multiplexing with...