...genomic variation. These joint efforts will produce a catalog of the impact of genomic variants on genome...Consortium investigators to examine how genomic variation affects genome function to influence phenotypes...Collections IGVF Consortium Impact of Genomic Variation on Function (IGVF) Consortium Collection Read ... opens in a new window) The Impact of Genomic Variation on Function (IGVF) Consortium (Link opens in a... a new window) aims to understand how genomic variation affects genome function, which in turn impacts...perturbs the genome to assess the impact of genomic variation on genome function and phenotype and shares their...

...alpha variable 27 TCRAV10S1, TCRAV27S1 TRAV29DV5 T cell receptor alpha variable 29/delta variable 5 (gene... immunoglobulin heavy variable group IGHV IGHV1-18 immunoglobulin heavy variable 1-18 IGHV118 IGHV1-2 ... immunoglobulin heavy variable 1-2 IGHV12 IGHV1-24 immunoglobulin heavy variable 1-24 IGHV124, VH IGHV1... immunoglobulin heavy variable 1-3 IGHV13 IGHV1-45 immunoglobulin heavy variable 1-45 IGHV145, VH IGHV1...immunoglobulin heavy variable 1-46 IGHV146 IGHV1-58 immunoglobulin heavy variable 1-58 IGHV158, VH IGHV1...immunoglobulin heavy variable 1-69 IGHV1-E, IGHV169, IGHV1E IGHV1-8 immunoglobulin heavy variable 1-8 IGHV18 IGHV1... heavy variable 1-C (provisional, non-functional) IGHV1C IGHV1-F immunoglobulin heavy variable 1-F (provisional...

...System Various Plasmids T7/lacO and lacO variants that can be expressed in B. subtilis Various combinations...protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent protein variant (YPet)...protein variant (YPet) optimized for FRET measurements with a cyan fluorescent protein variant (CyPet)...Gileadi containing a variety of tags can be found here . pBAD LIC Series (8-Series) Various Plasmids pBAD (...that come with various epitope tags useful for protein purification. pET LIC Series Various Plasmids T7-...Series Various Plasmids pTrc, hybrid of trp and lac promoters (Lactose/IPTG inducible) Various combinations...Series Various Plasmids pPrpB Propionate Jay Keasling Propionate inducible vectors with various resistances...

...Some important variables include: Type of genetic modification - CRISPR libraries vary based on whether...3rd 5 Varies hCRISPRi-v2 subpooled libraries 83971 — 83977 Inhibition Human Weissman 3rd 5 Varies mCRISPRa-v2...3rd 5 Varies mCRISPRi-v2 subpooled libraries 83989 — 83995 Inhibition Mouse Weissman 3rd 5 Varies Human...136478 & 136479 Inhibition Human Weissman 3rd Varies Varies Human CRISPRi-v2 Dual Guide Libraries 197348...221409 221410 Knockout Human Superti-Furga 3rd Varies Varies Human Transcription Factor Knockout Library ... edit Varies CRASP-Seq Gene KO Library 232069 Knockout Human Gonatopoulos-Pournatzis 3rd Varies 95,893...Library 101926 — 101934 Knockout Human Bassik 3rd 10 Varies Bassik Mouse CRISPR Knockout Library 1000000121...

...Single-chain fragment variables (scFvs) contain only the variable heavy chain and variable light chain regions... way to produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies can...cells). The genetic reorganization that creates variety in the antibody allowing for their extreme specificity...large amounts of a single antibody with little variation. Recombinant plasmids - Antibodies can be produced...the plasmid. This produces the least amount of variation between antibodies, allows for the antibody’s ...easily confirmed via sequencing, and enables the variable region and constant regions of the antibody to.... The tips of the Fab fragments contain highly variable regions, shown in light blue and light orange,...

...binding) SpCas9 VRER variant 3' NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9...LbCpf1) 5' TTTV AsCpf1 RR variant 5' TYCV LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter...out target genes in various cell types and organisms, but modifications to various Cas enzymes have extended...available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements, and...Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' ...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started...use, and there are several to choose from. Many variations of Cas9 exist, along with other Cas enzymes like...

...Mammalian, Varies pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant of lentiCRISPR... of interest in a controlled environment under various conditions. When choosing what plasmid backbone...expression vector from Gileadi lab Worm unc-54, variety of worm gene promoters C. elegans vector kit - ...C-terminal 2xFLAG tag in pCS for a variety of systems, including Xenopus HA... Backbones Neomycin (G418) Mammalian, Varies Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression...Thy1.1 - Lentiviral shRNA expression Gentamicin Varies pJL-TRBO - An improved agroinfection-compatible...virus (TMV)-based transient expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression...

... part of an adaptive immune system - utilizing various CRISPR-associated (Cas) genes to not only store...of bacterial genomes, are highly diverse, with varying PAM sequences and number/types of Cas proteins....Pseudomonas aeruginosa . Anti-CRISPR genes employ varied mechanisms to block CRISPR. Some prevent the CRISPR-Cas...specificity, orthogonality, and multiplexibility in various species, as well as the development of new applications...Homology-directed repair (HDR) allows us to create a variety of modifications in endogenous loci with unprecedented... High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very...specific, programmable platform well-suited for various biological and translational technologies. Biotechnology...

...table below lists gRNA plasmids designed to target various genes or genomic regions. It is highly recommended...gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and your genomic...genomic target may reduce the gRNA activity. The variety in sequence decreases the gRNA activity and increases...increases off-target effect. Which species or variant of Cas9 ( S. pyogenes , S. aureus etc.) was this gRNA...compatible with a single species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be...deposit process. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...

...that many of the articles linked below include variants with different binding affinities or negative-... Calcium Yellow protein calcium sensor jYCaMP1 variants (Constitutive or Cre-dependent) jYCaMP: an optimized...Int Ed Engl. 2018 Jun 27. Tetsuya Kitaguchi ATP Variants of ATP sensor iATPSnFR1.0 and iATPSnFR1.1 (cytoplasmic...Loren Looger Glutamine FLIPQTV3.0 glutamine sensor variants Visualization of glutamine transporter activities...Glutamate Ultrafast glutamate sensors (iGluSnFR variants) Ultrafast glutamate sensors resolve high-frequency...1720648115. Katalin Torok Glutamate Membrane displayed variants of fluorescent glutatmate sensors (iGluSnFR1) ...pH Superfolder pHluorin pH sensor A superfolder variant of pH-sensitive pHluorin for in vivo pH measurements...

...expression systems and variables for using CRISPR in mammalian cells. Some of the variables include: Species...tunable expression of Cas9 and/or gRNA in a wide variety of mammalian cell lines Useful for difficult to...modify prior to designing your gRNA, as sequence variation between your gRNA targeting sequence and target...cleavage. The number of alleles for each gene may vary depending on the specific cell line or organism,...to consider alternative Cas enzymes ( see Cas9 variants and PAM sequences ). Once possible PAM sequences...your experimental system. CRISPR efficiency will vary based on the method of delivery and the cell type...

... by tRNA variant that suppress the TAG nonsense codon, and tyrU gene replaced by tRNA variant inactivated...can help us understand and evolve proteins for various purposes from therapeutics to biopolymers. Tips...tR4-OMeYRS/GFP* tyrosyl-tRNA synthetase E. coli various unnatural amino acids Mammalian TAG Peter Schultz...Schultz 50832 pAcBac1.tR4-MbPyl PylRS M. barkeri variety of unnatural amino acids Mammalian TAG Peter Schultz...knocked out, tyrTV and tyrU genes replaced by tRNA variant that suppress the TAG nonsense codon). Expresses...knocked out, tyrTV and tyrU genes replaced by tRNA variant inactivated by the truncation of the anticodon ...

...Addgene's viral service. Control EGFP vectors in various serotypes for serotype testing: AAV1, AAV2, AAV5...that can be used to validate the performance of various serotypes before conducting larger scale experiments...Plasmid #37825) Description : Ready-to-use AAV in various serotypes suitable for direct injection. These ...Plasmid #50465) Description : Ready-to-use AAV in various serotypes suitable for direct injection. These ...

...information. Plasmids Expressing Spike and Spike Variants Full-length wildtype SARS-CoV-2 spike protein ...D614G change which is present in many circulating variants. The table below lists expression plasmids that...protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Addgene COVID-19 Resources and Collections COVID...

... base editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements...fusions, modifying the linker region between Cas variant and deaminase to adjust the editing window, or ...systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window...deaminase (AID). Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...

...Arabidopsis, wheat, maize, rice, tomato, and a variety of other species. In addition to individual plasmids...tables below highlight plasmids spanning a wide variety of molecular biology technologies: from genome ...selection of plasmids expressing different functional variants of Cas9 and empty gRNA backbones. A few examples...Daniel Voytas. A CRISPR/Cas9 Toolkit for use in a variety of plant species (e.g. maize and Arabidopsis) and...

...molecule is not required to be fluorescent. A variety of organic dyes and fluorescent proteins have been... Mammalian Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian...Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange...LSSmOrange mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan & Yellow ...

...miRNAs, and use a wide variety of drug selection markers. See article for more variations. Campeau 39481 pLenti-puro... See article for more cre-lox shRNA expression variants. Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion...fusion. See plasmids 36083 and 36086 for other FP variants. Tsoulfas 41393 pCW57.1 3rd Inducible lentiviral...

... short ~100 nt guide RNA ( gRNA ) that uses 20 variable nts at its 5’ end to base pair with a target genomic... and LbCpf1) for use in mammalian cells SpCas9 variants with no detectable off-target effects Modified...specifity of Staphylococcus aureus Cas9 (SaCas9 KKH variant) Engineered CRISPR-Cas9 nucleases with altered ...

...publications, focused on somatic variants found in lung adenocarcinoma as well as variants found across cancer types...Research Plasmids and Resources Addgene has a large variety of resources for use in cancer signaling studies...