...plating if your transformed cells are being plated on agar with ampicillin selection.  Notes and tips  We’...

...post, it is my pleasure to announce that we once again reached new heights on the Addgene blog: we surpassed...

... or emailing us at [email protected].  Thank you again for a fantastic year and happy reading. Best wishes...

...that cleaves DNA heteroduplexes, and run on an agarose gel to identify DNA fragments. PCR and restriction...the appropriate restriction enzyme and run on an agarose gel to identify DNA fragments. PCR amplification...insertions only). The PCR product is then run on an agarose gel to determine whether the edit was successful...

...work-life balance? Tom Ellis reveals “I don't. Ask me again in five years and hopefully I'll have worked something...

...access your tasks on the go Never miss a deadline again. Organize you experiments, keep track of upcoming...

...new year, it is a wonderful opportunity to once again reflect on our achievements in the last year and...

...information about limitations and caveats of Cre usage: Vagaries of conditional gene targeting. Schmidt-Supprian...

...SpyLigase can be used to link affibodies or antibodies against common tumor markers to subsequently capture cancerous...

...the Lab!" and more.   Acknowledgments Thank you again to Connie Cepko, Tom Ellis, and George Church for...

...targets. These pooled libraries include enrichment against kinases, cell cycle proteins, and more.  “In the...

...wonderfully nerdy art for sale, and photos of the Agar Art competition entries were displayed in a gallery...

...Nuclei are then incubated with a primary antibody against the protein of interest. The Protein A fused MNase...

...Cry proteins will then be screened for toxicity against local insect pests. By developing biological agents...

...undertake and every service we provide is tested against fulfilling our mission. “Addgene’s nonprofit mission...

... inside the cell, in the nucleus and cytoplasm. Again, they found that SunTag marked single molecules ...

... running a small aliquot of the reaction on an agarose gel. You should see bands with the appropriate ...

.../10.1038/s41589-020-0535-8 Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of ...

...Toolbox No Llamas Required - Synthetic Nanobodies Against Membrane Proteins More resources from addgene....

...of 5 day old fish. The larvae were immobilized in agar at the head with their tails still free, and different...